1,720,964 research outputs found

    PORCINE VEG PROTEINS AND TEAR PREALBUMINS

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    Small soluble proteins, belonging to the lipocalin family are secreted in large amounts by tongue von Ebner's glands and lachrymal glands. In humans, the lingual protein, called VEG, and the lachrymal protein, called tear prealbumin, have shown identical cDNA sequences. In the pig, we have purified homodimeric proteins with subunits of 17 kDa, both from von Ebner's glands and from lachrymal glands. In both cases, the proteins can be resolved into two isoforms on a chromatofocusing column. Partial aminoacid sequences and full cDNA sequences have been obtained for the more abundant forms purified from both tissues. The two proteins appear to be identical, as in humans. The reason why the same protein is expressed in different tissues, as well as its physiological function, still remain to be clarified

    Three odorant-binding proteins from rabbit nasal mucosa

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    Following the purification of an odorant-binding protein (OBP) from rabbit nasal mucosa, we have identified, purified and partially characterized two additional OBPs from the nasal tissue of the same animal species. OBP-II is a monomer of 21 kDa and isoelectric point 4.2; OBP-III is a dimer with subunits of 23 kDa and isoelectric point 4.8. Like OBP-I, both these new members bind the odorant 2-isobutyl-3-methoxypyrazine. The partial amino acid sequences of the three OBPs, determined by Edman degradation, confirm that they are members of the OBP family, but reveal poor similarity between them. However, higher similarity is found between each OBP and other members of the lipocalin family In particular, OBP-I is most similar to bovine OBP (55% identity in the N-terminal region), OBP-II is >50% identical, limited to its first 18 amino acids, to mouse OBP-I and porcupine OBP-II, while OBP-II shares 26 out of the first 40 amino acids with major urinary protein (MUP) 4, a member of the mouse salivary proteins. The possible role of these proteins in olfactory transduction is also discussed

    MULTIPLE TYPES AND FORMS OF ODORANT-BINDING PROTEINS IN THE OLD-WORLD PORCUPINE HYSTRIX-CRISTATA

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    1. Eight new proteins have been identified and purified from the nasal tissue of the old-world porcupine. 2. All of them show good binding activity to tritiated 2-isobutyl-3-methoxypyrazine. 3. They show values of molecular mass, in denaturing conditions, between 18 and 23 kDa, and of isoelectric points between 4.2 and 4.6. 4. This represents the first example of more than two odorant-binding proteins (OBPs) found in the same animal species and could support a discriminating function of these proteins in the process of odour perception

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    A stereoscopic augmented reality system for the veridical perception of the 3D scene layout

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    The recent diffusion of the stereoscopic 3D technologies has yielded the development of affordable and of everyday use devices for the visualization of such information. This has paved the way for powerful human and computer interaction systems based on augmented reality environment where humans can interact with both virtual and real tools. However, an observer freely moving in front of a 3D display could experience a misperception of the depth and of the shape of virtual objects. Such distortions can have serious consequences in scientific and medical fields, where a veridical perception is required, and they can cause visual fatigue in consumer and entertainment applications. Here, we propose a novel augmented reality system capable to correctly render 3D virtual objects, without adding significant delay, to an observer that changes his position in the real world and acts in the virtual scenario. The correct perception of the scene layout is assessed through two experimental sessions with several observers
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