1,720,994 research outputs found
Interferons induce xanthine dehydrogenase gene expression in L929 cells
No full text: Human interferon-alpha A/D (Bg/II) (IFN-alpha A/D) and mouse interferon-gamma (IFN-gamma) are shown to induce xanthine dehydrogenase (XD) mRNA in L929 fibroblastic cells. XD mRNA accumulation after IFN-alpha A/D treatment is relatively fast, being already evident after 4 h and reaching its maximum after 24 h. IFN-alpha A/D is active in inducing XD mRNA at 0.1 unit/ml and it is maximally active at 10(3) units/ml. The half-life of the XD message is unaffected by IFN-alpha A/D treatment, whereas the transcriptional activity of the XD gene and the concentrations of XD heterogeneous nuclear RNA are increased by 2- and 6-fold respectively. The effect of IFN-alpha A/D on XD mRNA is insensitive to cycloheximide, suggesting that protein synthesis de novo is not required. Experiments conducted with specific inhibitors suggest that protein kinase C, cyclic AMP and arachidonic acid metabolites derived from lipoxygenase or cyclooxygenase do not act as second-messenger molecules in the induction of XD mRNA by IFN-alpha A/D. XD mRNA is also induced in NIH3T3 fibroblastic cells, but not in F9 teratocarcinoma or B16 melanoma cells after treatment with IFN-alpha A/D. NIH3T3 are the only cells so far tested that have detectable XD and xanthine oxidase activities under basal conditions and after IFN-alpha A/D treatment, although their responsiveness to the cytokine is much less than that observed in L929 cells
Purification and characterization of mouse liver xanthine oxidase.
No full textXanthine oxidase (EC 1.1.3.22) is purified to homogeneity from mouse liver after
induction with bacterial lipopolysaccharide. The enzyme has an apparent molecular
weight of 300,000 in its native state and it is suggested to be constituted of
two identical subunits of Mr 150,000 each. The isoelectric point is 6.7 and the
apparent Km value for xanthine is 3.4 microM. The amino acid composition of mouse
xanthine oxidase is quite similar to that of Drosophila xanthine dehydrogenase
The ATRA-21 gene-expression model predicts retinoid sensitivity in CEBPA double mutant, t(8;21) and inv(16) AML patients
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Il santuario di Apollo Pythios a Gortyna: nuovi dati sulle fasi protogeometriche e geometriche
No full textNel contributo sono prese in esame le testimonianze ceramiche di età protogeometrica e geometrica provenienti da diversi settori del santuario di Apollo Pythios a Gortina di Creta, indagato tra il 2014 e il 2019 dall’Università degli Studi di Padova e dalla Scuola Archeologica Italiana di Atene. L’analisi di questi materiali ha permesso di acquisire nuovi dati sulla frequentazione del santuario nelle sue fasi pi antiche che precedono la monumentalizzazione dell’area e, di conseguenza, di aprire nuove prospettive per lo studio delle origini del luogo di culto, in correlazione con il fenomeno di formazione del centro urbano di Gortina
Studies on the active site of human cytidine deaminase by site-directed mutagenesis on its complementary DNA
No full textPurification and characterization of mouse liver xanthine oxidase
No full text: Xanthine oxidase (EC 1.1.3.22) is purified to homogeneity from mouse liver after induction with bacterial lipopolysaccharide. The enzyme has an apparent molecular weight of 300,000 in its native state and it is suggested to be constituted of two identical subunits of Mr 150,000 each. The isoelectric point is 6.7 and the apparent Km value for xanthine is 3.4 microM. The amino acid composition of mouse xanthine oxidase is quite similar to that of Drosophila xanthine dehydrogenase
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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