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Plasma-membrane proteins in water-stressed sunflower seedlings
In sunflower seedlings either regularly watered or subjected to water stress by withholding water for 5 days, plasma membrane was isolated by partitioning in an aqueous polymer two-phase system. In the stressed seedlings an osmotic adjustment of 0.1 MPa took place. Water deficit conditions determined an increase in membrane permeability and in the recovery of plasma membrane proteins. Total and soluble protein levels decreased by 9.4 and 45.8% respectively in the stressed seedlings. The sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) of the soluble proteins and plama membrane proteins did not show qualitative changes in the protein patterns of both the control and the stressed seedlings. Several quantitative differences in the levels of plasma membrane proteins were observed: the 60, 56 and 37 KDa bands, as well as the 37 to 20.1 KDa group, reached higher levels under stress. These last polypeptides can be identified as peripheral plasma membrane proteins of the bilayer
Applicabilità dell’elettroforesi per la classificazione e conservazione del germoplasma di specie erbacee.
Effect of salinity on water relations, sodium accumulation, chlorophyll content and proteolytic enzymes in a wild wheat
Inhibition by ATP and other regulatory properties of glutamate decarboxylase from wheat embryos
Antioxidant function of phytic acid in seeds evidence from a maize lpa (low phytic acid) mutant
Succinic semialdehyde dehydrogenase in higher-plants - purification and properties of the enzyme from Triticum durum embryos.
Enzymatic and non-enzymatic protective mechanisms in recalcitrant seeds of Araucaria bidwillii subjected to desiccation
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