86,639 research outputs found

    Enhancing inter-subject reproducibility in insulin bioavailability measurements through real-time calibration

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    A real-time calibration to enhance the inter-subject reproducibility of insulin bioavailability measurements was proposed and validated on a diabetic patient with more than 49000 impedance values. The measurement method monitors drug absorption through a transducer consisting of a sensitive material (human abdominal tissue) and an impedance spectrometer. The in-vivo experiments revealed a statistically significant negative second-order polynomial trend in impedance magnitude corresponding to the amount of rapid-acting insulin injected. To manage the uncertainty inherent in the sensing block, the method integrates real-time calibration. Implementing a real-time calibration method based on a customized model identification at each insulin administration resulted in a reduction in deterministic error by a factor of 18. A further reduction in deterministic error for clinical application (46%) was achieved with a customized parameter second-order polynomial model with respect to the customized parameter linear model. Lastly, when less conductive long-acting insulin was administered, an increasing trend in impedance magnitude was observed, aligned with the theoretical framework of the proposed method

    Evidence of protein secretion by cultured pachytene spermatocytes.

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    Pachytene spermatocytes isolated from immature rat testis were cultured for 6 h in the presence of [35S]methionine and the macromolecules present in the culture medium were analyzed by one-dimensional and two-dimensional gel electrophoresis. The electrophoretic profiles obtained showed a limited number of polypeptides, some of them consisting of families of spots with the same molecular weight but a different isoelectric point. The reproducibility of the results and the unaltered metabolic activity of the cells during culture time, indicate that the macromolecules present in the medium do not represent degradative products of the cells. Part of the detected macromolecules are considered secreted proteins since the addition of monensin to the cells induces their disappearance from the culture medium
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