1,720,961 research outputs found
IN VITRO DEVELOPMENTAL COMPETENCE OF HORSE OOCYTES WITH DIFFERENT CUMULUS MORPHOLOGY
Full text linkIn vitro production of equine embryos by intracytoplasmic sperm injection (ICSI) is gaining interest, and both commercial and research applications have rapidly increased worldwide. Oocyte collection from excised ovaries is generally performed using one of two methods: aspiration and scraping. Aspiration has been found to give a lower recovery rate [1] and to yield oocytes largely denuded of cumulus, when compared to scraping [2]. For commercial programs, oocytes are typically recovered from live mares using transvaginal ultrasound- guided aspiration. Despite the high number of oocytes with only corona radiata (CR) collected by aspiration (in vivo or ex vivo), most studies classify horse oocytes simply as having a compact (CP) or expanded (EX) cumulus, without considering oocytes with only CR. In the only study [3] classifying horse oocytes as having CP, EX, or partial cumulus investments, no data on embryo production were available. The aim of this study was to investigate the embryo developmental ability after ICSI of horse cumulus-oocyte complexes (COCs) presenting only CR as compared to CP and EX COCs. Horse oocytes were collected by follicular aspiration of abattoir-derived ovaries. After classification into EX, CP or CR COCs, they were in vitro matured for 26 h in DMEM-F12 supplemented with 10% heat-inactivated fetal bovine serum (FBS), 50 ng/ml epidermal growth factor, 100 ng/ml insulin-like growth factor 1, 0.1 IU/mL pFSH-LH (Pluset). At the end of the maturation period oocytes were denuded and classified as mature, immature or degenerate. MII oocytes were fertilized by piezo-drill ICSI using frozen-thawed semen from the same stallion, and in vitro cultured in SOF medium for 7.5 days. Culture medium was refreshed every 3 days and on day 6 of culture 5% FBS was added. At day 7.5 of in vitro culture, embryos were stained with 1 μg/mL bisbenzimide fluorescent dye (Hoechst 33342) to assess the number of nuclei and classify them. Maturation rate, cleavage rate and morula/blastocyst rates were recorded; data were statistically analysed using a Chi Square test (IBM SPSS Statistics 23) and significance was assessed for P<0.05. The experiment included 14 replicates. A total of 611 oocytes were used. Overall maturation rate was 60.2%. MII, immature and degenerate oocyte rates were not statistically different (P>0.05) among different COC morphologies (MII 61.7% vs. 57.6% vs. 59.3% for EX, CP and CR COCs respectively). Cleavage rate was lower (P<0.05) for CR (42.1%) compared to CP (55.6%) but not significantly different from EX (54.3%), while morula/blastocyst development after 7.5 days of culture was similar (P>0.05) among groups (12.0% vs. 8.9% vs. 14.7% of injected oocytes for EX, CP and CR COCs respectively). In conclusion, even if CR COCs show a lower cleavage rate after ICSI, their developmental ability is similar to CP and EX COCs, demonstrating that they can be used as a useful source of embryos in the horse
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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