207,348 research outputs found
Amyloid Assembly Endows Gad m 1 with Biomineralization Properties
No full textAcid proteins capable of nucleating Ca2+ and displaying aggregation capacity play key roles in the formation of calcium carbonate biominerals. The helix-loop helix EF-hands are the most common Ca2+-binding motifs in proteins. Calcium is bound by the loop region. These motifs are found in many proteins that are regulated by calcium. Gad m 1, an Atlantic cod β-parvalbumin isoform, is a monomeric EF-hand protein that acts as a Ca2+ buffer in fish muscle; the neutral and acid apo-forms of this protein can form amyloids. Since Ca2+-nucleating proteins have a propensity to form extended β-strand structures, we wondered whether amyloid assemblies of an EF-hand protein were able to influence calcium carbonate crystallization in vitro. Here, we used the Gad m 1 chain as a model to generate monomeric and amyloid assemblies and to analyze their effect on calcite formation in vitro. We found that only amyloid assemblies alter calcite morphology
Intern experience at CH���M Hill, Inc.: an internship report
No full textIncludes author's vita"Submitted to the College of Engineering of Texas A&M University in partial
fulfillment of the requirement for the degree of Doctor of Engineering."Includes bibliographical referencesA review of the author's internship experience with CH���M HILL, Inc.
during the period September 1975 through May 1976 is presented. During this nine month
internship the author worked as an Engineer II in the Industrial Processes discipline of this
large consulting engineering firm... The author's prime responsibility was as one of three
lead design engineers on the design of a large wastewater treatment facility for a pulp mill
in Hoquiam, Washington owned by ITT Rayonier Inc. The work generally consisted of the design
of individual treatment units and associated piping and pumping. The purpose of the project
was to provide wastewater treatment capabilities that would satisfy the effluent limitations
(standards) imposed upon the mill by the State of Washington Department of Ecology and the
U.S. Environmental Protection Agency. The author's assignment also entailed necessary
interaction with the project manager and other CH���M HILL design engineers and support staff
members, the client's representatives, and representatives of two other consulting engineering
firms working on the project. Thus, the internship position at CH���M HILL provided considerable
experience coordinating the author's work with the work of other engineers, guiding the design
and administrative efforts of a support staff, and interacting regularly with the client and
other consulting firms. This broad exposure to a variety of engineering and organizational
problems provided a valuable educational experience
NMR analysis of Gad m 1: scFv-gco9 complex formation.
No full text<p>(A) Selected regions of the <sup>1</sup>H/<sup>15</sup>N HSQC spectra of Gad m 1 in its free form (blue) and in complex with scFv-gco9 at 40 μM or 70 μM complex concentration in red and green, respectively. (B) CSP index as a function of amino acid residue numbers was used to map the residues involved in the interaction in two complex concentrations, 40 μM (red) and 70 μM (gray). (C) Primary sequence of Gad m 1.0202 showing mapped amino acid residues (red) and amino acid residues whose NMR signals disappeared upon complex formation (yellow). (D) Same as in panel C colored in the Gad m 1 NMR solution structure (PDB:2mbx). (E) <sup>15</sup>N backbone relaxation parameters R<sub>2</sub>/R<sub>1</sub> of Gad m 1 and the Gad m 1:scFv-gco9 complex as a function of amino acid residue numbers.</p
PENAFSIRAN GAD AL-BASAR MENURUT SAYYID QUTB DAN M. QURAISH SHIHAB
No full textFenomena saat ini, banyak terjadi pelecehan seksual, yang disebabkan
karena kurangnya menjaga pandangan dan mengumbar aurat serta tidak
mengenakan penutup badan dengan sempurna sesuai syariat Islam. Dalam skripsi
yang berjudul “Penafsiran Gad Al-Basar Menurut Sayyid Qutb dan M. Quraish
Shihab”. Penulis memfokuskan pada dua ayat surat an-Nur, yaitu kata “yaguddu
pada an-Nur ayat: 30 dan kata yagd}ud}na pada ayat: 31. Jenis penelitian ini
menggunakan metode kualitataif, penelitian yang bersumber Library Research.
Yakni, dengan mengumpulkan data-data yang bersangkutan terkait makna gad albas
ar, baik berupa karya ilmiah, skripsi, tesis, maupun lainnya. Dan juga dengan
mencari kitab Tafsir Fī Ẓilal Al-Qur’an dan al-Misbah sebagai acuan utama dalam
penelitian ini. Dilanjutkan dengan menganalisis makna gad al-bas}ar dari kedua
tokoh tersebut dengan masing-masing kitab Tafsirnya.
Penelitian ini berakhir dengan menyimpulkan sebagai jawaban rumusan
masalah. Tiga rumusan masalah yang penulis paparkan, pertama, bagaimana
penafsiran makna gad} al-bas}ar menurut Sayyid Qutb di dalam Fī Zilal Al-Qur’an
dan M. Quraish Shihab dengan Tafsirnya al-Misbah? Kedua, apa perbedaan dan
persamaan penafsiran Sayyid Qutb dan M. Quraish Shihab tentang makna gad albas
ar? Ketiga, apa kelebihan dan kekurangan penafsiran Sayyid Qut}b dan M.
Quraish Shihab?
Hasil dari penelitian yang telah penulis lakukan terkait makna gad} albas}
ar menurut kedua mufassir tersebut tidak jauh berbeda, yakni banyak akan
kesamaannya. Terlihat dari metode yang digunakan kedua tokoh tersebut sama,
yaitu metode tahlili. Adapaun perbedaan antar kedua tokoh tersebut terdapat
pada cara menafsirkan, yaitu Sayyid Qutb yang cenderung pada penggunaan
sastra Arab. Berbeda dengan M. Quraish Shihab, ia lebih dengan memahami ayat
secara kontekstual dan menafsirkan dengan perkata. Lain dari itu, perbedaan juga
terlihat dari lingkungan dan pengaruh sosial yang berbeda. Sedangkan pada
kekurangan dan kelebihan dari kedua Tafsir tersebut hanya pada pengaruh sosial
yang terjadi pada saat kitab Tafsir kedua tokoh tersebut dituliskan
Flash memories using minimum push up, multi-cell and multi-permutation schemes for data storage
No full textRank modulation has been recently proposed as a scheme for storing information in flash memories. Three improved aspects are disclosed. In one aspect the minimum push-up scheme, for storing data in flash memories is provided. It aims at minimizing the cost of changing the state of the memory. In another aspect, multi-cells, used for storing data in flash memories is provided. Each transistor is replaced with a multi-cell of m transistors connected in parallel. In yet another aspect, multi-permutations, are provided. The paradigm of representing information with permutations is generalized to the case where the number of cells in each level is a constant greater than one.U
Simulation of thermal plant optimization and hydraulic aspects of thermal distribution loops for large campuses
No full textFollowing an introduction, the author describes Texas A&M University and its utilities system. After that, the author presents how to construct simulation models for chilled water and heating hot water distribution systems. The simulation model was used in a $2.3 million Ross Street chilled water pipe replacement project at Texas A&M University. A second project conducted at the University of Texas at San Antonio was used as an example to demonstrate how to identify and design an optimal distribution system by using a simulation model. The author found that the minor losses of these closed loop thermal distribution systems are significantly higher than potable water distribution systems. In the second part of the report, the author presents the latest development of software called the Plant Optimization Program, which can simulate cogeneration plant operation, estimate its operation cost and provide optimized operation suggestions. The author also developed detailed simulation models for a gas turbine and heat recovery steam generator and identified significant potential savings. Finally, the author also used a steam turbine as an example to present a multi-regression method on constructing simulation models by using basic statistics and optimization algorithms. This report presents a survey of the author??s working experience at the Energy Systems Laboratory (ESL) at Texas A&M University during the period of January 2002 through March 2004. The purpose of the above work was to allow the author to become familiar with the practice of engineering. The result is that the author knows how to complete a project from start to finish and understands how both technical and nontechnical aspects of a project need to be considered in order to ensure a quality deliverable and bring a project to successful completion. This report concludes that the objectives of the internship were successfully accomplished and that the requirements for the degree of Degree of Engineering have been satisfied
"Reflections on the subject of Emigration from Europe with a view to Settlement in the United States" By M. Carey.
No full text"Reflections on the subject of Emigration from Europe with a view to Settlement in the United States: containing bried sketches of the moral and political character of those states.
By M. Carey, member of the American philosophical, and of the American Antiquarian Society, and author of The Olive Branch, Cindiciae Hibernicae, essays on banking, on political economy, and on internal improvement.
To which are now added the English editor's comments on the subject; together with Important Advice to Emigrants, and Cautions Against Impositions Practiced in the Outports
M-regularity and the fourier-mukai transform
No full textThis is a survey of M-regularity and its applications, expanding on lectures given by the second author at the Seattle conference, in August 2005, and at the Luminy workshop "Geometrie Algebrique Complexe", in October 2005
Neurological syndromes associated with anti-gad antibodies
No full textGlutamic acid decarboxylase (GAD) is an intracellular enzyme whose physiologic function is the decarboxylation of glutamate to gamma-aminobutyric acid (GABA), the main inhibitory neurotransmitter within the central nervous system. GAD antibodies (Ab) have been associated with multiple neurological syndromes, including stiff-person syndrome, cerebellar ataxia, and limbic encephalitis, which are all considered to result from reduced GABAergic transmission. The pathogenic role of GAD Ab is still debated, and some evidence suggests that GAD autoimmunity might primarily be cell-mediated. Diagnosis relies on the detection of high titers of GAD Ab in serum and/or in the detection of GAD Ab in the cerebrospinal fluid. Due to the relative rarity of these syndromes, treatment schemes and predictors of response are poorly defined, highlighting the unmet need for multicentric prospective trials in this population. Here, we reviewed the main clinical characteristics of neurological syndromes associated with GAD Ab, focusing on pathophysiologic mechanisms
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