1,721,350 research outputs found
NMR analysis of Gad m 1: scFv-gco9 complex formation.
No full text<p>(A) Selected regions of the <sup>1</sup>H/<sup>15</sup>N HSQC spectra of Gad m 1 in its free form (blue) and in complex with scFv-gco9 at 40 μM or 70 μM complex concentration in red and green, respectively. (B) CSP index as a function of amino acid residue numbers was used to map the residues involved in the interaction in two complex concentrations, 40 μM (red) and 70 μM (gray). (C) Primary sequence of Gad m 1.0202 showing mapped amino acid residues (red) and amino acid residues whose NMR signals disappeared upon complex formation (yellow). (D) Same as in panel C colored in the Gad m 1 NMR solution structure (PDB:2mbx). (E) <sup>15</sup>N backbone relaxation parameters R<sub>2</sub>/R<sub>1</sub> of Gad m 1 and the Gad m 1:scFv-gco9 complex as a function of amino acid residue numbers.</p
Amyloid Assembly Endows Gad m 1 with Biomineralization Properties
Get PDFAcid proteins capable of nucleating Ca2+ and displaying aggregation capacity play key roles in the formation of calcium carbonate biominerals. The helix-loop helix EF-hands are the most common Ca2+-binding motifs in proteins. Calcium is bound by the loop region. These motifs are found in many proteins that are regulated by calcium. Gad m 1, an Atlantic cod β-parvalbumin isoform, is a monomeric EF-hand protein that acts as a Ca2+ buffer in fish muscle; the neutral and acid apo-forms of this protein can form amyloids. Since Ca2+-nucleating proteins have a propensity to form extended β-strand structures, we wondered whether amyloid assemblies of an EF-hand protein were able to influence calcium carbonate crystallization in vitro. Here, we used the Gad m 1 chain as a model to generate monomeric and amyloid assemblies and to analyze their effect on calcite formation in vitro. We found that only amyloid assemblies alter calcite morphology
Gad M. Jacobsohn Family Collection 2004
No full textThe collection contains family trees of the Bratz and Michel families from the early 18th to mid 20th century, with some birth and death dates and locations.digitize
Amyloid Assembly Endows Gad m 1 with Biomineralization Properties
No full textAcid proteins capable of nucleating Ca2+ and displaying aggregation capacity play key roles in the formation of calcium carbonate biominerals. EF-hands are among the largest Ca2+-binding motif in proteins. Gad m 1, an Atlantic cod &beta;-parvalbumin isoform, is a monomeric EF-hand protein that acts as a Ca2+ buffer in fish muscle and is able to form amyloids under acidic conditions. Since nucleating Ca2+ protein have a propensity to form extended &beta;-strand structures, we wondered whether amyloid assemblies of a protein containing refolded EF-hand motifs were able to influence the in vitro calcium carbonate crystallization. Here we have used the Gad m 1 chain as model to generate monomeric and amyloid assemblies and analyze their effect on in vitro calcite formation. We found that only amyloid assemblies alter calcite morphology.</jats:p
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
On Indeterminate Strings Matching
Get PDFGiven two indeterminate equal-length strings p and t with a set of characters per position in both strings, we obtain a determinate string p_w from p and a determinate string t_w from t by choosing one character per position. Then, we say that p and t match when p_w and t_w match for some choice of the characters. While the most standard notion of a match for determinate strings is that they are simply identical, in certain applications it is more appropriate to use other definitions, with the prime examples being parameterized matching, order-preserving matching, and the recently introduced Cartesian tree matching. We provide a systematic study of the complexity of string matching for indeterminate equal-length strings, for different notions of matching. We use n to denote the length of both strings, and r to be an upper-bound on the number of uncertain characters per position. First, we provide the first polynomial time algorithm for the Cartesian tree version that runs in deterministic (nlog² n) and expected (nlog nlog log n) time using (nlog n) space, for constant r. Second, we establish NP-hardness of the order-preserving version for r=2, thus solving a question explicitly stated by Henriques et al. [CPM 2018], who showed hardness for r=3. Third, we establish NP-hardness of the parameterized version for r=2. As both parameterized and order-preserving indeterminate matching reduce to the standard determinate matching for r=1, this provides a complete classification for these three variants
Order-Preserving Squares in Strings
Get PDFAn order-preserving square in a string is a fragment of the form uv where u ≠ v and u is order-isomorphic to v. We show that a string w of length n over an alphabet of size σ contains (σn) order-preserving squares that are distinct as words. This improves the upper bound of (σ²n) by Kociumaka, Radoszewski, Rytter, and Waleń [TCS 2016]. Further, for every σ and n we exhibit a string with Ω(σn) order-preserving squares that are distinct as words, thus establishing that our upper bound is asymptotically tight. Finally, we design an (σn) time algorithm that outputs all order-preserving squares that occur in a given string and are distinct as words. By our lower bound, this is optimal in the worst case
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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