1,721,097 research outputs found
Analisi genetica e molecolare della tolleranza allo stress idrico in piante coltivate: ruolo dell'ormone acido abscissico
No full textPRODUZIONE DI LINEE TRASFORMATE DI ARABIDOPSIS CHE SOVRAESPRIMONO UN GENE DI ORZO INDUCIBILE DA STRESS
No full textIsolamento e localizzazione di geni coinvolti nella risposta allo stress termico ed idrico in piante coltivate mediante l'utilizzo di metodologie genetico-molecolari.
No full textLocating genes in wheat which regulate production of the drought-stress hormone abscisic acid.
No full textSYBR GREENER Real-Time PCR to detect almond in traces in processed food
No full textBecause food ingredients are sometimes considered as causative factors in IgE mediated food allergies, DNA-based tests may prove to be very useful to establish whether allergenic species have been used
in foodstuffs production. The development of two SYBRGreenERTM Real-Time PCR assays, targeting Pru1 and rbcL genes, based on melting curve analysis, to detect allergen species in food has been presented. Applicability of these methods was assessed with several commercial products containing processed almond
Quantitative detection method for Roundup Ready soybean in food using duplex real-time PCR MGB chemistry
No full textBACKGROUND: Methodologies that enable the detection of genetically modified organisms (GMOs) (authorized and nonauthorized)
in food and feed strongly influence the potential for adequate updating and implementation of legislation together
with labeling requirements. Quantitative polymerase chain reaction (qPCR) systemswere designed to boost the sensitivity and
specificity on the identification of GMOs in highly degraded DNA samples; however, such testing will become economically
difficult to cope with due to increasing numbers of approved genetically modified (GM) lines. Multiplexing approaches are
therefore in development to provide cost-efficient solution.
RESULTS: Construct-specific primers and probe were developed for quantitative analysis of Roundup Ready soybean (RRS)
event glyphosate-tolerant soybean (GTS) 40-3-2. The lectin gene (Le1) was used as a reference gene, and its specificity was
verified. RRS- and Le1-specific quantitative real-time PCR (qRTPCR) were optimized in a duplex platform that has been validated
with respect to limit of detection (LOD) and limit of quantification (LOQ), as well as accuracy. The analysis of model processed
food samples showed that the degradation of DNA has no adverse or little effects on the performance of quantification assay.
CONCLUSION: In this study, a duplex qRTPCR using TaqMan minor groove binder-non-fluorescent quencher (MGB-NFQ)
chemistry was developed for specific detection and quantification of RRS event GTS 40-3-2 that can be used for practical
monitoring in processed food products
Yeast toxicogenomics: a system biology approach to study the response to 5-fluorouracil and nystatin.
No full text
Transgenic plants as a tool for studying regulation of gene expression in responce to environmental stress
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