1,721,202 research outputs found
The potential role of the archaeobotany in the archaeological sciences: the case study of Neolithic Vitis vinifera L. carpological remains.
Helichrysum italicum (Roth) G. Don essential oil: composition and potential antineoplastic effect.
The antitumor effect of Helichrysum species has been rarely documented in literature. Thus, in the present
work, the potential antineoplastic properties of the essential oil extracted from Helichrysum italicum (Roth)
G. Don flower heads by hydrodistillation were investigated. The biochemical profile of the essential oil was
characterized by GCMS analysis: Neryl acetate (33.97%), a-Pinene (28.50%), Nerol (7.97%), Neryl phenylacetate (7.11%) and b-Caryophyllene (5.71%) were the most abundant molecules of the phytocomplex. An absolute quantitation of five terpens (D-Limonene, b-Linalool, a-Terpineol, Nerol, and Neryl acetate) was also
carried out, to further typify the oil chemotype. The antiproliferative effect of the essential oil was assessed
on B16F10 murine melanoma cells, by Trypan Blue exclusion test and MTT assay. These preliminary analyses
demonstrated that H. italicum essential oil was able to significantly inhibit tumor cell growth, in a dose and
time-dependent manner, inducing only low levels of cytotoxicity. Probably, the bioactivity of this oil against
the B16F10 cells was due to its elevated antioxidant power, which was measured by three different in vitro
tests (DPPH, ABTS and FRAP). According to all these results, H. italicum essential oil might be considered a
promising natural source of new anticancer compound
Microsatellite analysis of Latial Olea europaea L. cultivars
Olea europaea L. is one of the oldest domesticated tree species. O. europaea varieties cannot be confused because they are very different in morphology, genetics, and secondary metabolite content. It is important to study and establish the genetic structure of vegetal cultivars to better distinguish them, to solve past misclassification, to preserve plant biodiversity, and to increase their use, diffusion, selection, resistance to adversities, marketing, and scientific applications. Five simple sequence repeat loci (DCA-3, DCA-9, UDO99-9, UDO99-35, and EMO-3) were used to differentiate 39 individuals, representing 13 olive cultivars sampled in Latium (Central Italy). The markers showed a high discrimination power and were able to differentiate 39 alleles. Observed heterozygosity ranged from 0.538 at locus UDO99-9 to 1 at locus UDO99-35, with a mean value of 0.784. DCA loci were the most informative ones. Sample clustering, based on their genetic distance and similarity values, produced a phylogenetic network that has shown a unique major group of cultivars, composed of two sub-branches, and two independent taxa
Developmental dynamics of Arbutus unedo L. flower: morphological and molecular evidence.
Going Beyond Counting First Authors in Author Co-citation Analysis
The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Evidence for alphavbeta3 and alphavbeta5 integrin-like vitronectin (VN) receptors in Candida albicans and their involvement in yeast cell adhesion to VN.
Gallic acid as a phytostimulant enhancing yield and quality of Mentha spicata L. under deficit- and well-watered conditions
Here, we propose gallic acid (GA) as a phytostimulant for Mentha spicata subsp. spicata L. (spearmint), an aromatic herb of great global economic importance. Our evidence would suggest the potential of GA in promoting the acclimation of spearmint to drought stress (DS), also improving yield and quality of its essential oil (EO). We observed that GA and DS did not induce significant morphometric (e.g., stem length) and physiological (e.g., redox state, water content) modifications, indicating an innate tolerance of M. spicata to both these factors. However, spectrophotometric, HPLC-DAD and GC-MS analyses revealed that GA tended to induce the accumulation of some phytochemicals (e.g., terpenoids), mainly responsible for fragrance, quality and bioactivity of EO. Additionally, plants exposed to GA increased the number of peltate glandular trichomes and their content in mono- and sesqui-terpenes. Finally, we demonstrated that DS determined a reduction of spearmint EO yield, while GA significantly favoured it, especially at low doses, both in deficit- and well-watered conditions. Since an increase of GA concentration in roots and/or leaves of the treated plants was not observed, we hypothesised that the proposed phytostimulant exerted its activity indirectly, that is by modulating rhizobiota and/or soil structure and features or triggering signalling transduction pathways starting from the rhizodermis
- …
