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    Description of the puparium and other notes on the morphological and molecular identification of Phthitia empirica (Diptera, Sphaeroceridae) collected from animal carcasses

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    Background: A correct identification of species is fundamental in order to derive all the biological, ecological, and behavioral information useful to reconstruct previous events when insects are used in forensic and funerary archaeological contexts. Little attention was paid in the past to the description of the immature stages of flies, especially of species belonging to family found only occasionally on human cadavers and animal carrion. Sphaeroceridae species belong to this category. Results: In this work, adult and puparia of Phthitia empirica (Hutton, 1901) are illustrated and described. In addition, some observations about the molecular identification of this species are presented and discussed. Conclusions: This work will allow the identification of puparia of this species providing additional tools in forensic entomological investigation. More in general, the effort of producing morphological descriptions and molecular information of species of potential forensic interest clearly implements the possibility to provide a deeper understanding of the peri- and post-mortem events in violent crimes

    A New, Non-Invasive Methodology for the Molecular Identification of Adult Sarcophagidae from Collections

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    Correct species identification is the cornerstone of all scientific studies that involve insects. Alongside traditional morphological identification techniques, molecular identification based on the characterization and analysis of specific mitochondrial or nuclear gene regions is becoming commonplace. Despite the good results that can be achieved, DNA extraction usually involves invasive techniques that lead to the partial or total destruction of specimens. In this work, a non-invasive DNA extraction technique is described. The technique was tested on the abdomens of dry-preserved Sarcophagidae (Diptera) specimens collected between 1889 and 2015. This allowed for the correct identification of species without impairing diagnostic morphological structures useful for further studies

    State of the art of the funerary archaeoentomological investigations in Italy

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    Archaeoentomology is a branch of environmental archaeology focusing on insects and other arthropods contributing, with other disciplines, to reconstruct a more complete view of past events, climate and landscape. Funerary archaeoentomology focuses on the arthropods associated with human remains or with elements part of funerary rituals such as offerings. In the last years, several papers have been published on Italian material covering around 5000 years. Sardinian necropolis, Egyptian mummies and offerings stored in public collections are among the oldest samples in which insects have been evidenced. Mummified and skeletonised bodies of kings, Saints and common people are another important source of insects in addition to remains of WWI soldiers. The arthropod remains found during these investigations belong mainly to Diptera (flies), Coleoptera (beetles) and Arachnida (mites). The aim of this paper is to summarise the Italian data about insects associated with human remains of archaeological interest in order to increase the interest and the utilisation of a discipline too often underestimated
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