1,721,045 research outputs found
Thiago Regis Longo Cesar da Paixão and Subrayal M. Reddy (Eds.): Materials for chemical sensing
No full textA Model for a Correct Evaluation of the Sensitivity, Detection Limit and Selectivity Constants of Ion Selective Electrodes
No full textAdvances in molecular analysis of biomarker for autoimmune and carcinogenic diseases
No full textBiomarkers are cellular or molecular mediators or responders that can serve as indicators of normal biological processes, pathologic processes, or responses to specific pharmacologic treatment. Identification of new biomarkers for specific diseases and the development of novel strategies for prognosis, diagnosis, and treatment designed to take advantage of diagnostic and prognostic indicators is of paramount importance not only for clinical chemistry but also for other types of medicine, for example internal medicine and, obviously, for patients. On the basis of these considerations, the development of easy, non-invasive, and reliable methods for early detection of malignant tumors is a matter of great concern; a specific interest nowadays is the search for biomarkers enabling early identification and distinction between indolent tumors and more aggressive forms of cancer capable of spreading and leading to metastasis. Autoimmune diseases (ADs) arise from an overactive immune response of the body against substances and tissues normally present in the bod
New competitive dendrimer-based and highly selective immunosensor for determination of atrazine in environmental, feed and food samples: The importance of antibody selectivity for discrimination among related triazinic metabolites
No full textA new voltammetric competitive immunosensor selective for atrazine, based on the immobilization of a
conjugate atrazine-bovine serum albumine on a nanostructured gold substrate previously functionalized
with poliamidoaminic dendrimers, was realized, characterized, and validated in different real samples
of environmental and food concern. Response of the sensor was reliable, highly selective and suitable
for the detection and quantification of atrazine at trace levels in complex matrices such as territorial
waters, corn-cultivated soils, corn-containing poultry and bovine feeds and corn flakes for human use.
Selectivity studies were focused on desethylatrazine, the principal metabolite generated by long-term
microbiological degradation of atrazine, terbutylazine-2-hydroxy and simazine as potential interferents.
The response of the developed immunosensor for atrazine was explored over the 10−2–103 ng mL−1
range. Good sensitivity was proved, as limit of detection and limit of quantitation of 1.2 and 5 ng mL−1,
respectively, were estimated for atrazine. RSD values <5% over the entire explored range attested a good
precision of the device
Electrochemically induced derivatization of poly(2,2′-bithiophene) and characterization of functionalized polymers by FT-IR microscopy, SEM microanalysis and EQCM
No full textExtraction of electrolytes from acqueous solutions and their spectrophotometric determination by use of acid-base chromoionophores in lipophylic solvents
No full text
Multiplex liquid chromatography-tandem mass spectrometry for the detection of wheat, oat, barley and rye prolamins towards the assessment of gluten-free product safety
Full text linkCeliac patients should feel confident in the safety of foods labelled or expected to be gluten-free. In this context, a targeted proteomic approach based on liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) technique was proposed to assess the presence of celiotoxic cereals, namely wheat, oats, barley and rye, in raw and processed food products. To this aim, unique marker peptides were properly selected in order to distinguish between the different cereal types. A revised cocktail solution based on reducing and denaturing agents was exploited for prolamin extraction from raw and processed food; in addition, defatting with hexane was carried out for sample clean-up, allowing to largely reduce problems related to matrix effect. Method validation on fortified rice flour showed good analytical performance in terms of sensitivity (limits of detection in the 2-18 mg kg(-1) range). However, poor trueness was calculated for self-made incurred bread (between 3 and 30% depending on the peptide), probably due to baking processes, which reduce gluten extractability. Thus, it is evident that in the case of processed foods further insights into sample treatment efficiency and reference materials for protein calibration are required to obtain accurate gluten determination. Finally, the developed method was applied for the analysis of market food products, offering the possibility to discriminate among cereals, with good agreement with labelled ingredients for gluten-containing foodstuffs
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