1,720,969 research outputs found
A general strategy to identify mimotopes of pathological antigens using only random peptide libraries and human sera.
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Characterization of monoclonal antibodies (mAb) from mice immunized with HCV hypervariable region 1 (HVR1) mimotopes.
No full textDerivation of vaccines from mimotopes. Immunologic properties of Human Hepatitis B Virus Surface Antigen mimotopes displayed on filamentous phage.
No full textDerivation of vaccines from mimotopes. Immunologic properties of Human Hepatitis B Virus Surface Antigen mimotopes displayed on filamentous phage.
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Recognition by human sera and immunogenicity of HBsAg mimotopes selected from an M13 phage display library.
No full textWe used two mouse monoclonal antibodies (mAb) specific for the human hepatitis B virus surface antigen (HBsAg) to screen a random peptide library of 15 amino-acid residues displayed as a fusion to protein III of filamentous phage M13. By a combination of affinity selection, immuno-screening and ELISA techniques, we selected peptides that are recognized by the anti-HBsAg mAb and show aa similarity with the natural antigen. The selected phage-displayed epitopes (phagotopes) behave as antigenic mimics of HBsAg. One phagotope is specifically recognized by human sera from HBsAg-immunized individuals, pointing to the possible use of phagotopes as markers to detect the presence of specific Ab in the serum. The same phagotope also elicits Ab directed against HBsAg in mice, indicating that mAb-selected phagotopes can also be immunogenic mimics of the natural antigen. These findings demonstrate that it is possible to identify disease-specific epitopes that can be used as diagnostic reagents and as leads for the development of acellular vaccines
A general strategy to identify mimotopes of pathological antigens using only random peptide libraries and human sera.
No full textSelection of Antigenic and Immunogenic Mimics of Hepatitis C Virus Using Sera from Patients
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