1,720,971 research outputs found
Effects of hydrogen peroxide on both proliferative capacity and vitality of different animal species lymphocytes
No full textPlasma protein changes in horse after prolonged physical exercise: A proteomic study.
No full textPhysical exercise induces various stress responses and metabolic adaptations that have not yet been completely elucidated. Novel biomarkers are needed in sport veterinary medicine to monitor training levels and to detect subclinical conditions that can develop into exercise-related diseases. In this study, protein modifications in horse plasma induced by prolonged, aerobic physical exercise were investigated by using a proteomic approach based on 2-DE and combined mass spectrometry procedures. Thirty-eight protein spots, associated with expression products of 13 genes, showed significant quantitative changes; spots identified as membrane Cu amine oxidase, α-1 antitrypsin, α-1 antitrypsin-related protein, caeruloplasmin, α-2 macroglobulin and complement factor C4 were augmented in relative abundance after the race, while haptoglobin β chain, apolipoprotein A-I, transthyretin, retinol binding protein 4, fibrinogen γ chain, complement factor B and albumin fragments were reduced. These results indicate that prolonged physical exercise affects plasma proteins involved in pathways related to inflammation, coagulation, immune modulation, oxidant/antioxidant activity and cellular and vascular damage, with consequent effects on whole horse metabolism
Liver antioxidant systems adaptation in carvacrol supplemented farmed seabrass
No full textThe present study was performed to evaluate the antioxidant activities of carvacrol in vivo on farmed fish. TBARS, protein Carbonil groups and SOD were assessed in liver of seabass fed with essential oil supplemented diet (0.025% and 0.05% of carvacrol). TBARS and CO were reduced by both the two treatments tested, while SOD activity was unaffected. The results indicate carvacrol as a promising dietary supplement in organic aquaculture in respect of the current European Community framework rules based on animal welfare and environmental preservation
Association Analysis Between DNA Markers and Number of Functional Teats in Italian Large White Pigs
No full textThe aim of this work was to study the association between five single-nucleotide polymorphisms (SNPs), each in one of five genes (CTSL, EPOR, ESR2, GPX5, and STAT5B), and functional teat number (FTNUM). Each marker was genotyped in at least 940 Italian Large White sows. All SNPs segregated in the analyzed breed with minor allele frequencies ranging from 0.18 (CTSL and EPOR) to 0.40 (ESR2). The genotyped sows were categorized into two classes based on their FTNUM: FTNUM equal to 14 (59% of the samples) and FTNUM greater than 14 (41%). Differences in allele frequencies between the two classes were statistically significant (P < 0.01) for the GPX5 polymorphism
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Ovine subclinical mastitis: Proteomic analysis of whey and milk fat globules unveils putative diagnostic biomarkers in milk
No full textimpact on both animal welfare and economy in the dairy industry. A better knowledge is
needed to understand the ovine mammary gland metabolism and its response to bacterial
infection. In this study, the proteomic changes in ovine milk as a result of subclinical mastitis
were investigated by comparing both whey and fat globule membrane profiles of samples
from Staphylococcus chromogenes-positive individuals, with those from non-infected counterparts
having high or lowsomatic cell count; the latterwere used as control. 2-DEand combined
MS procedures were utilized for this purpose. Although sample bromatological parameters
were very similar, proteomic analysis highlighted significant differences between the three
experimental groups. Most relevant changes were observed between samples of infected milk
and control. Modifications related to the defense response of the mammary gland to the
pathogen were evident, with important consequences on nutritional and technological
properties of milk. On the other hand, quantitative protein changes between non-infected
samples with low and high levels of somatic cells indicated that the latter may result as a
consequence of a probable unpaired cellular metabolism due to cellular stress, hormonal
variations or previous infections. Putative biomarkers useful for the monitoring of sheep
mammary metabolismand for the careful management of ovine subclinical mastitis to avoid
its clinical degeneration are proposed and discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
PROTEOMIC ANALYSIS OF MULTI DRUG RESISTANCE IN STREPTOCOCCUS UBERIS: A PRELIMINARY STUDY
No full textThe aim of this study was to assess the molecular mechanism of multidrug resistance in S. uberis isolated from bovine mastitic milk by using a proteomic gel‐based approach and combined MS procedure
- …
