1,720,987 research outputs found
Antimutagenic and Antioxidant Activity of a Selected Lectin-free Common Bean (Phaseolus vulgaris L.) in Two Cell-based Models
No full textLegumes and particularly beans are a key food of Mediterranean diet representing an important source of proteins, fiber, some minerals and vitamins and bioactive compounds. We evaluated the antioxidant and anti-mutagenic effects of a new fermented powder of a selected lectin-free and phaseolamin-enriched variety of common bean (Phaseolus vulgaris L.), named Lady Joy. Lady Joy lysate (Lys LJ) was studied in human erythrocytes and in Saccharomyces cerevisiae yeast cells. The antioxidant and anti-hemolytic properties of Lys LJ, studied in an ex vivo erythrocytes system using the cellular antioxidant assay (CAA-RBC) and the hemolysis test, evidenced a dose-dependent antioxidant activity as well as a significant hemolysis inhibition. Besides, results evidenced that Lys LJ treatment significantly decreased the intracellular ROS concentration and mutagenesis induced by hydrogen peroxide in S. cerevisiae D7 strain. In conclusion, Lys LJ showed both an antimutagenic effect in yeast and a strong scavenging activity in yeast and human cells
Beneficial effects of natural products: new candidates for feed additives
No full textIn recent years, the need for food safety and animal welfare has stimulated the search for new feeding strategies based on the use of natural feed additives, capable of ensuring the health of animals and excellence in their production performance.
This study evaluates the phytochemical composition, antioxidant activity, and anti-hemolytic effects of a new product, Lisosan Reduction, containing the grain lysate Lisosan G and extracts of Picrorhiza and Desmodium.
The data obtained were compared with those related to Lisosan G alone. Phytochemical compounds are present in high quantities in both products. The flavonoids, in particular, are doubled in Lisosan Reduction compared to Lisosan G. Lisosan Reduction also showed a greater antioxidant capacity, determined by the ORAC test, compared to Lisosan G. The antioxidant activity of Lisosan G and Lisosan Reduction was also evaluated in an ex-vivo system of human and dog erythrocytes (CAA-RBC). The results of this test show a higher antioxidant effect of the mixture Lisosan Reduction, compared to the lysate alone, both in human and canine samples, with greater effectiveness in human erythrocytes.
The anti-hemolytic effects of Lisosan G and Lisosan Reduction were also evaluated, and the results show a stronger effect of Lisosan Reduction, compared with Lisosan G, in both human and dog samples. The results of our study demonstrate that Lisosan G and, in particular, Lisosan Reduction contain several bioactive compounds and have a strong antioxidant activity, suggesting the use of these compounds as feed additives to improve animal health
Relationship among IL-6, LDL cholesterol and lipid peroxidation
No full textPrevious studies evidenced a significant reduction in serum cholesterol levels during an episode of acute inflammation. The aim of the present study was to verify the hypothesis of a regulatory role of cytokines through an in vitro model that simulates a situation of vascular inflammation and high levels of LDL or lipoperoxides.
Human microvascular endothelial cells-1 were used in all experiments. The cells were exposed for 24 h to increasing doses of LDL, oxidized lipoprotein, and 8-isoprostane (in the absence or presence of SQ29.548, a TXA2 receptor antagonist). Moreover, LDL receptor and oxidized lipoprotein receptor expression analyzed after endothelial cells’ incubation with increasing doses of interleukin-6. The ELISA test and quantitative real-time PCR were performed. Endothelial cells showed a significant increase in interleukin-6 medium levels associated with LDL, oxidized LDL and with the degree of oxidation (absence or presence of SQ29.548), while 8-isoprostane did not. Treatment of human microvascular endothelial cells-1 for 24 h with increasing doses of interleukin-6
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Antioxidant and anti-inflammatory effect of a fermented powder of Lady Joy bean in primary rat hepatocytes
No full textThe role and beneficial effects of plant and food extracts against various diseases induced by oxidative stress have received much attention in recent years. Legumes are rich in bioactive compounds and some studies suggest a correlation between their consumption and a reduced incidence of diseases. Primary cultures of rat hepatocytes were used to investigate whether and how an extract obtained from a fermented powder of bean named Lady Joy (Phaseolus vulgaris L.) is able to regulate antioxidant and detoxifying enzymes through the NRF2 pathway, inhibit NF-kB activation and reduce H2O2-induced ER stress. All of the antioxidant and detoxifying enzymes studied were significantly up-regulated by Lady Joy treatment. Western blot showed that Nrf2 was activated by Lady Joy treatment. Also, cells treated with this fermented bean were partially protected against NF-kB activation resulting from H2O2 stress. As a link between oxidative stress and endoplasmic reticulum dysfunction is hypothesized, we verified whether Lady Joy was able to protect cells from H2O2-induced ER stress, by studying the response of the proteins CHOP, Bip and Caspase12. The results of this study show that Lady Joy can induce Nrf2 pathway, inhibits NF-kB and protects ER from stress induced by H2O2
Nutraceuticals and cardiovascular risk: potential role of EPCs modulation
Full text linkAccording to WHO cardiovascular diseases (CVDs) are the first cause of death in the world: more people die annually from CVDs than from any other cause. Vascular endothelium plays a pivotal role in the onset and the progression of these pathologies and cardiovascular risk factors are frequently associated to the levels of endothelial progenitor cells (EPCs), bone marrow-derived circulating progenitors for the endothelial lineage.
Since EPCs not only preserve vascular endothelium homeostasis, but directly participate to re-endothelization and neovascularization, these cells represent an emerging actor in vascular competence and thus a cell model of great interest.
An unhealthy diet is one of the main cardiovascular risk factor; there is indeed a great interest in the potential protective effects of "nutraceuticals,", food-derived compounds that exert beneficial effects on human and animal health. The characterization of the endothelial effects of different nutraceuticals may provide fresh insights into their potential role in CVDs prevention. Several studies have already showed the protective effects of natural antioxidants on EPCs levels and functionality; some examples are resveratrol, catechin and folic acid.
Fermentation has recently shown interesting roles in cardiovascular prevention since this process created a new class of food, rich in bioactive compounds, the fermented food.
Consumption of fermented legumes and cereals, but also fermented beverages (such as beer and wine) was found to protect endothelial function through lipid-lowering, anti-inflammatory and antioxidative mechanisms. Little is known about the effects of fermentation-derived nutraceuticals on EPCs and given the important role of this cardiovascular biomarker, further analysis in this field can improve CVDs prevention and treatment
- …
