955 research outputs found
Anreizsysteme als Führungsinstrument
No full textBecker FG. Anreizsysteme als Führungsinstrument. In: Kieser A, Reber G, Wunderer R, eds. Handwörterbuch der Führung. Enzyklopädie der Betriebswirtschaftslehre. Vol 10. 2. neu gestaltete und erg. Aufl. . Stuttgart: Schäffer-Poeschel; 1995: 34-46
A. Baud, G. Cornu, M. Martiniani-Reber, J.-M. Poisson, J.-F. Reynaud & C. Treffort, Saint-Rambert. Un culte régional depuis l'époque mérovingienne. Histoire et archéologie
No full textMasset Claude. A. Baud, G. Cornu, M. Martiniani-Reber, J.-M. Poisson, J.-F. Reynaud & C. Treffort, Saint-Rambert. Un culte régional depuis l'époque mérovingienne. Histoire et archéologie. In: L'Homme, 1996, tome 36 n°139. p. 187
In Vitro reconstitution and imaging of microtubule dynamics by fluorescence and label-free microscopy
No full text© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Hirst, W. G., Kiefer, C., Abdosamadi, M. K., Schäffer, E., & Reber, S. In Vitro reconstitution and imaging of microtubule dynamics by fluorescence and label-free microscopy. STAR Protocols, 1(3), (2020): 100177, doi:10.1016/j.xpro.2020.100177.Dynamic microtubules are essential for many processes in the lives of eukaryotic cells. To study and understand the mechanisms of microtubule dynamics and regulation, in vitro reconstitution with purified components has proven a vital approach. Imaging microtubule dynamics can be instructive for a given species, isoform composition, or biochemical modification. Here, we describe two methods that visualize microtubule dynamics at high speed and high contrast: (1) total internal reflection fluorescence microscopy and (2) label-free interference reflection microscopy.We thank the AMBIO imaging facility (Charité, Berlin) and Nikon at MBL for imaging support. We thank all former and current members of the Reber lab for discussion and helpful advice, in particular Christoph Hentschel and Soma Zsoter for technical assistance. S.R. acknowledges funding by the IRI Life Sciences (Humboldt-Universität zu Berlin, Excellence Initiative/DFG). W.H. was supported by the Alliance Berlin Canberra co-funded by a grant from the Deutsche Forschungsgemeinschaft (DFG) for the International Research Training Group (IRTG) 2290 and the Australian National University. C.K. thanks the Deutsche Forschungsgesellschaft (DFG, JA 2589/1-1). C.K. and M.A. thank Steve Simmert and Tobias Jachowski former and current members of the Schäffer lab
Affinity purification of label-free tubulins from xenopus egg extracts
No full text© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Reusch, S., Biswas, A., Hirst, W. G., & Reber, S. Affinity purification of label-free tubulins from xenopus egg extracts. STAR Protocols, 1(3), (2020): 100151, doi:10.1016/j.xpro.2020.100151.Cytoplasmic extracts from unfertilized Xenopus eggs have made important contributions to our understanding of microtubule dynamics, spindle assembly, and scaling. Until recently, these in vitro studies relied on the use of heterologous tubulin. This protocol allows for the purification of physiologically relevant Xenopus tubulins in milligram yield, which are a complex mixture of isoforms with various post-translational modifications. The protocol is applicable to any cell or tissue of interest.
For complete details on the use and execution of this protocol, please refer to Hirst et al. (2020).This article was prompted by our stay at the Marine Biological Laboratory (MBL), Woods Hole, MA, in the summer of 2016 funded by the Princeton-Humboldt Strategic Partnership Grant together with the lab of Sabine Petry (Princeton University). We are grateful to the National Xenopus Resource (NXR) for supplying frogs. For mass spectrometry, we would like to acknowledge the assistance of Benno Kuropka and Chris Weise from the Core Facility BioSupraMol supported by the Deutsche Forschungsgemeinschaft (DFG). We thank the Protein Expression Purification and Characterization (PEPC) facility at the MPI-CBG; in particular, we thank Aliona Bogdanova and Barbara Borgonovo. We thank all former and current members of the Reber lab for discussions and helpful advice, in particular Christoph Hentschel and Soma Zsoter for technical assistance. S.R. acknowledges funding from the IRI Life Sciences (Humboldt-Universität zu Berlin, Excellence Initiative/DFG). W.H. was supported by the Alliance Berlin Canberra co-funded by a grant from the Deutsche Forschungsgemeinschaft (DFG) for the International Research Training Group (IRTG) 2290 and the Australian National University
Implicit Learning and Tacit Knowledge: an Essay on the Cognitive Unconscious
No full textIn this new volume in the Oxford Psychology Series, the author presents a highly readable account of the cognitive unconscious, focusing in particular on the problem of implicit learning. Implicit learning is defined as the acquisition of knowledge that takes place independently of the conscious attempts to learn and largely in the absence of explicit knowledge about what was acquired. One of the core assumptions of this argument is that implicit learning is a fundamental, “root” process, one that lies at the very heart of the adaptive behavioural repertoire of every complex organism. The author's goals are to outline the essential features of implicit learning that have emerged from the many studies that have been carried out in a variety of experimental laboratories over the past several decades, to present the various alternative perspectives on this issue that have been proposed by other researchers and to try to accommodate these views with his own, to structure the literature so that it can be seen in the context of standard heuristics of evolutionary biology, to present the material within a functionalist approach and to try to show why the experimental data should be seen as entailing particular epistemological perspectives, and to present implicit processing as encompassing a general and ubiquitous set of operations that have wide currency and several possible applications. Chapter 1 begins with the core problem under consideration in this book, a characterization of “implicit learning” as it has come to be used in the literature. Reber puts this seemingly specialized topic into a general framework and suggests a theoretical model based on standard heuristics of evolutionary biology. In his account, Reber weaves a capsule history of interest in and work on the cognitive unconscious. Chapter 2 turns to a detailed overview of the experimental work on the acquisition of implicit knowledge, which currently is of great interest. Chapter 3 develops the evolutionary model within which one can see learning and cognition as richly intertwining issues and not as two distinct fields with one dominating the other. Finally, Chapter 4 explores a variety of entailments and speculations concerning implicit cognitive processes and their general role in the larger scope of human performanc
“À moda de”: notas acerca de En singeant...: pastiches littéraires, de Sérgio Milliet e Charles Reber
No full textEm 1918 Sérgio Milliet lançou, com Charles Reber, En singeant...: pastiches littéraires, segundo livro de sua lavra em solo suíço (um ano antes, saíra Par le sentier pela editora Le Carmel). O autor publicaria mais duas obras antes de retornar ao Brasil: Le départ sous la pluie e Œil de bœuf. O objetivo deste estudo é, à luz das reflexões sobre o pastiche propostas por Genette, Decout e Aron, apresentar – passando em revista o contexto de publicação e o rol de homenageados com as imitações – comentários acerca de um título que permanece desconhecido dos leitores e ignorado pela crítica.In 1918 Sérgio Milliet released, with Charles Reber, En singeant...: pastiches littéraires, the second work he wrote in Switzerland (a year before the publishing house Le Carmel had published Par le sentier). The author published two other volumes before returning to Brasil: Le départ sous la pluie and Œil de bœuf. The purpose of the present study is to present comments about a literary work remains unknown to readers and ignored by critics. The said study will be guided by the reflections on pastiche proposed by Genette, Decout and Aron, and will review the context of publication and the list of those honored by the pastiche imitations
Minimal requirements for antiphospholipid antibodies ELISAs proposed by the European Forum on antiphospholipid antibodies.
No full textMinimal requirements for antiphospholipid antibodies ELISAs proposed by the European Forum on antiphospholipid antibodies
No full textAntiphospholipid ELISAs are part of the Antiphospholipid Antibodies Syndrome classification criteria, having the same diagnostic value as lupus anticoagulant. However, sometimes their results appear scarcely meaningful especially when wide metanalyses studies are performed, probably because of their well-known inter-laboratory variability. The application of a common protocol was shown to improve the test reproducibility, but this observation did not have any influence on the routine performances. After discussion among experts at the European level, we identified four conditions named "minimal requirements" considered useful to decrease the inter-laboratory variability: (1) to run the samples in duplicate; (2) to determine the cut off level in each laboratory analysing at least 50 samples from normal subjects, possibly age- and sex-matched with the patient population usually attending the Centre; (3) to calculate the cut-off level in percentiles; (4) to use stable external control in the tests. A collaborative study involving 36 European centres proved that the use of monoclonal anti-beta2 glycoprotein I antibodies, HCAL (IgG) and EY2C9 (IgM) as standards, can help to reduce the inter-laboratory coefficient of variation both in anticardiolipin (aCL) and anti-beta2GPI (anti-beta2 glycoprotein I) ELISA. Therefore, we propose HCAL and EY2C9 as external controls, but other monoclonal or polyclonal preparations may be considered. During an interactive workshop held last May in Italy, 16 companies producing these tests agreed to consider the introduction of the "requirements" in their products. We suggest to adopt these "requirements" particularly in clinical studies, in order to compare more easily the literature data
A multicenter evaluation of a new quantitative highly sensitive D-dimer assay for exclusion of venous thromboembolism.
No full textProposals for the measurement of anti-beta2-glycoprotein I antibodies.Standardization group of the European Forum on Antiphospholipid Antibodies.
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