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    Identification of epidemic “flavescence dorée” molecular variants in Emilia-Romagna and Veneto regions

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    The study of the genetic variability of phytoplasmas is a fundamental tool to clarify their epidemiology and to implement an effective monitoring and management of their associated diseases. “Flavescence dorée” (FD), a threatening disease of grapevine associated to phytoplasmas belonging to 16SrV group and their insect vector(s), is distributed within the most important European wine-producing areas, and has severe effects on both vineyard productivity and landscape management. FD is a quarantine disease in Europe, mainly transmitted by the ampelophagous leafhopper Scaphoideus titanus and, despite the efforts to contain the pathogen dissemination, the disease is still epidemic in several viticultural areas of Northern Italy. Based on sequence and restriction fragment length polymorphism (RFLP) analysis of the 16S rRNA gene two FD ribosomal groups were described as present in Italy during the last century: 16SrV-C and 16SrV-D. However, the sequencing of non-ribosomal loci, such as secY, map and rpsC, allowed the identification over the years of several variants within the FD phytoplasma populations. A multilocus analysis approach was carried out on symptomatic FD-infected samples collected in Emilia-Romagna and Veneto regions, in different areas where the disease is spreading in the last 2-3 years. The geographic distribution of the two strains was confirmed to be different, with areas (Treviso province) with the prevalence of FD-C and others (Verona and Modena provinces) with only FD-D strains presence. Interestingly, FD-D phytoplasmas were identified for the first time also in a vineyard located in Modigliana (Forlì-Cesena province) and surrounded by forests. The molecular analyses allowed the identification of genetic variants among FD-D populations in both regions, that is quite a novel finding for these phytoplasmas that showed, since their first molecular identification in 1996, very little variability. In particular, the sequencing of secY amplicons highlighted the presence of the same SNP in samples of cultivars Teroldego and Trebbiano collected in Verona province and in grapevines cultivar Sangiovese located in Modigliana (Forlì-Cesena province). On the other hand, among the FD-C phytoplasma strains, the highest variability was shown on rpsC gene, that highlighted 5 restriction profiles after RFLP analyses on samples from cultivar Glera collected in Treviso and Belluno provinces. The epidemiology of the disease is therefore still to be monitored, especially since it is now involving different vectors/plant hosts species that are very likely responsible for the emergence of these FD variants. Therefore, a continuous and capillary monitoring of the presence and emergence of FD strains associated with the disease in the areas where it is present is necessary for the application of the most appropriate and stringent control measures aimed to avoid their epidemic spreading

    Flavescenza dorata, nuova epidemia in Veneto?

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    I fitoplasmi della vite associati alla presenza di giallumi in Italia so- no stati studiati dal 2017 nell’am- bito del progetto europeo H2020 TROPICSAFE (Malattie associate alla presenza di procarioti e trasmesse da insetti in colture arboree in aree tro- picali e subtropicali). Le malattie studiate sono giallume letale della palma da cocco; giallu- me della vite e «huanglongbing» degli agrumi («citrus greening»), tutte gravi malattie infettive che solo di recente sono state riconosciute e studiate. Il progetto è finalizzato ad una loro ge- stione efficace, efficiente e sostenibile per ottenere la quale è necessario col- mare importanti lacune scientifiche. Il progetto cui partecipano dodici na- zioni (Italia, Spagna, Slovenia, Dani- marca, Regno Unito, Ghana, Sudafrica, Cile, Cuba, Messico, Giamaica e Francia (Guadalupa) è coordinato dalla profes- soressa Assunta Bertaccini dell’Alma Mater Studiorum - Università di Bolo- gna. Alcuni dei risultati delle ricerche sui giallumi della vite in collaborazio- ne con Istituzioni, colleghi e studenti sono riassunti nelle brevi note che se- guono e forniscono un aggiornamen- to sulla situazione della malattia in alcune delle aree viticole italiane più importanti

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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