1,721,020 research outputs found
Biomonitoring of human exposure to methyl bromide by isotope dilution mass spectrometry of peptide adducts
No full textA procedure for the determination of haemoglobin adducts formed by exposure to methyl bromide was evaluated as a possible method for the measurement of a biological index of exposure to the alkylating agent. The reaction products after in vitro incubation were used to design the chemical syntheses of deuterated peptides corresponding to the tryptic peptides where the modified residues had been identified. These peptides were used as standards for the quantitative evaluation of real samples, The correlation coefficient was r = 0.998 in the range 2.5-20 ppm. The relative standard deviation was about 3%, Blood samples were digested with trypsin and the mixture was analysed by liquid chromatography/electrospray mass spectrometry through selected ion monitoring of the mass signal relative to the modified peptides;, The analysis of blood samples from workers exposed to methyl bromide demonstrated the usefulness of this mass spectrometric-based method fur the monitoring of human exposure to the genotoxic alkylating agent via the synthesis of suitable peptide standards. This procedure is the first alternative method to the wed established monitoring of N-terminal adducts, the latter not being applicable to all alkylating agent
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Identification of hormonogenic tyrosines in fragment 1218-1591 of bovine thyroglobulin by mass spectrometry - Hormonogenic acceptor TYR-1291 and donor TYR-1375
No full textA fragment of bovine thyroglobulin encompassing residues 1218-1591 was prepared by limited proteolysis with thermolysin and continuous-elution polyacrylamide gel electrophoresis in SDS. The reduced and carboxymethylated peptide was digested with endoproteinase Asp-N and fractionated by reverse-phase high performance liquid chromatography. The fractions were analyzed by electrospray and fast atom bombardment mass spectrometry in combination with Edman degradation. The post-translational modifications of all seven tyrosyl residues of the fragment were characterized at an unprecedented level of definition. The analysis revealed the formation of: 1) monoiodotyrosine from tyrosine 1234; 2) monoiodotyrosine, diiodotyrosine, triiodothyronine (T-3), and tetraiodothyronine (thyroxine, T-4) from tyrosine 1291; and 3) monoiodotyrosine, diiodotyrosine, and dehydroalanine from tyrosine 1375. Iodothyronine formation from tyrosine 1291 accounted for 10% of total T-4 of thyroglobulin (0.30 mol of T-4/mol of 660-kDa thyroglobulin), and 8% of total T-3 (0.08 mol of T-3/mol of thyroglobulin). This is the first documentation of the hormonogenic nature of tyrosine 1291 of bovine thyroglobulin, as thyroxine formation at a corresponding site was so far reported only in rabbit, guinea pig, and turtle thyroglobulin. This is also the first direct identification of tyrosine 1375 of bovine thyroglobulin as a donor residue. It is suggested that tyrosyl residues 1291 and 1375 may support together the function of an independent hormonogenic domain in the mid-portion of the polypeptide chain of thyroglobulin
La costruzione del trauma nella guerra di Libia (1911-1912), in G. Mamone, F. Milazzo (a cura di), Storia e psichiatria. Problemi, ricerche, fonti, Milano, Biblion, 2019, pp. 159-172.
No full textQuelli a cavallo tra la fine dell’Ottocento e il primo Novecento sono anni decisivi per la definizione dell’auto-rappresentazione del genere maschile. In questo senso, per esempio, la guerra di Libia del 1911-1912 ha rappresentato un nodo cruciale non soltanto per l’espansione coloniale liberale, ma anche per il profondo rinnovamento della maschilità all’interno della società italiana. È sufficiente pensare alla codificazione del maschio dominante e civilizzatore che tanto ha contribuito alla proiezione del genere, o alle frustrazioni sessuali disattese al contatto con l’alterità libica. Una guerra coloniale che è centrale anche nell’ambito della storia della psichiatria. Il contributo mira a rivalutare la portata dell’esperienza nella Quarta Sponda, considerandola un laboratorio psichiatrico fondamentale e precedente l’immane conflitto mondiale. Una guerra dal potenziale traumatico inaspettato e violento, assai poco studiata dal punto di vista delle sue derive mentali. Rispolverando la casistica medica dedicata all’impresa tripolina, l'autore ne rivela le caratteristiche psicopatologiche principali, valorizzandone le caratteristiche peculiari in rapporto alla costruzione del trauma. Derive psichiatriche leggibili attraverso le produzioni orali e scritte dei militari coinvolti, vittime dimenticate di incontrovertibili processi di derealizzazione. Una lacuna storiografica per una guerra di importanza centrale per le sorti della contemporaneità italiana, un vuoto interpretativo che il saggio contribuisce a colmare
The “dark side” of b-lactoglobulin: Unedited structural features suggest unexpected functions.
No full textDispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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