1,721,028 research outputs found
Strong intracellular bioluminescence in G-Luc+KDEL transgenic HepG2 cells.
No full text(A) Experimental scheme of the cytotoxicity test presented in this study. (B) IVIS luminescence images of transgenic HepG2 clones: G-WT4, a HepG2 cell clone expressing G-WT; G+KDEL12, a clone expressing G+KDEL. Cells exhibiting strong G-Luc activity (red or yellow) were observed only in the G+KDEL12 variant clone, reflecting the enzyme’s intracellular localization. (C) Ratio of luminometric activities of G-Luc in medium vs. cells, obtained using a microplate reader. Medium/Cells ratio was calculated after 48 hr culture. The significant reduction in the Medium/Cells ratio indicates that the transgenic clones expressed cytoplasmic G-Luc (n = 3 wells for each, **p 30-fold higher bioluminescent signals than G-WT in transgenic HepG2 cells (n = 3 wells for each of four clones, **p < 0.001).</p
G-Luc+KDEL is highly stable in culture medium of damaged HepG2 cells.
No full textMedium was collected at five time points 24 hr after the treatment of G+KDEL-expressing HepG2 cells with a high concentration (0.5 μM) of the proteasome inhibitor MG132, and G-Luc activity was measured on a microplate reader.</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Five luciferase expression vectors and the properties of G-Luc, C-Luc, and their variants in HepG2 cells.
No full text(A) Experimental scheme of the cytotoxicity test presented in this study. (B) Luciferase expression vectors prepared in this study, initially characterized by transient expression in HepG2 cells. Blue: ORFs of G-WT or C-WT; orange: KDEL-encoding sequences listed in Table 1; yellow: SP, native signal peptide-encoding sequences; INS, insulator sequence; CAG, CAG promoter isolated from vector pCAGGS. (C) IVIS luminescence imaging of HepG2 cells expressing G+KDEL or WT-G. The blue signal indicates intracellular G-Luc activity. The absence of a signal in the “Medium” sample of G+KDEL-expressing cells indicates that the activity of secreted G-Luc in the culture medium was below the measurable limit. (D) IVIS luminescence imaging of HepG2 cells expressing C-Luc variants. Medium had higher C-Luc activity (red or yellow) than cells (blue). (E) Ratio of luminometric activities in medium vs. cells, obtained using a microplate reader after 48 hr culture. The reduction in the Medium/Cells ratio reflects the conversion of G-Luc and/or C-Luc from the naturally secreted form to a cytoplasmic form. (F) Extinction of luminometric activity in HepG2 cells expressing C-∆SP+KDEL, detected using a microplate reader (n = 4 wells for each, **p < 0.001). RLU: relative light units. (G) Strong intracellular luminometric activity in HepG2 cells expressing G+KDEL, detected using a microplate reader.</p
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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