18,607 research outputs found
S-Adenosyl-l-Methionine: a strategy for overcoming uL3-mediated drug resistance in p53 deleted colon cancer cells
No full textIn the field of anticancer therapy, re-sensitizing tumor cells to the drugs by exploiting novel strategies
is a promising approach to overcome the drug resistance with consequent improvement in clinical treatment.
In this context, to investigate novel therapeutic approaches taking advantage from anticancer and
antiproliferative effects of natural compounds, we focused our attention on S-adenosyl-L-methionine
(AdoMet), a ubiquitous and naturally occurring sulfonium compound. We tested its potential in overcoming
chemoresistance observed in our model of colon cancer cells lacking functional p53 and characterized by
low expression levels of uL3[1,2].
Here, we demonstrated that AdoMet exerts its cytotoxic activity in these cells and, more importantly,
restores their sensitivity to 5-fluorouracil (5-FU) treatment. In particular, AdoMet exposure impairs cell cycle
progression inducing cell cycle arrest at the S phase, which was associated with a significant increase of
Cyclin E and a decrease of Cyclin D. Furthermore, AdoMet inhibits autophagy, increases the production of
reactive oxygen species, and finally activates the caspase cascade, triggering the apoptotic pathway.
To best of our knowledge, the present study unveils, for the first time, the capability of AdoMet to resensitize
drug resistant colon cancer cells indicating this natural compound as a potential therapeutic agent
for colon cancer cells showing p53 and uL3 lower levels.
[1] A. Pecoraro, P. Carotenuto, G. Russo, A. Russo. Sci Rep. (2019), 9, 15431.
[2] A. Pecoraro, P. Carotenuto, B. Franco, R. De Cegli, G. Russo, A. Russo. Int J Mol Sci. (2020), 21, 2143
Lightcurve synthesis of the semi-detached binaries L T Her, WX Eri, A W Cam
No full textWe have analyzed, by means of a Roche-model-based method for lightcurve synthesis, the photometric observations of the eclipsing binaries L T Her, WX Eri, A W Cam. All systems are found to be serni-detached, with a main sequence primary and a lobe filling evolved secondary
High performance scientific computing using distributed infrastructures. Results and scientific applications derived from the italian PON ReCaS project
No full textThe book aims to provide a deep look into ialian actions taken in some fields of science and high performance computing (HPC), and the italian effort to bridge the gap with respect to Europe. The italian PON ReCaS Project is written for graduate readers and professionals in the field of high performance computing. It presents and discusses innovative and important technological solutions, and describes interesting resuklts in variou fields of application.ReCaS stands for "Rete di Calcolo ad Alte Prestazioni per SuperB e altre applicazioni" and is a computing network infrastructure in Southern Italy devoted to scientific and non-scientific applications within the vision of a common europeamn infrastructure for computing, stiorage and network
miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells
No full textMicroRNAs (miRNAs) play an important
role in proper function and differentiation of mouse
embryonic stem cells (ESCs). We performed a systematic
comparison of miRNA expression in undifferentiated
vs. differentiating ESCs. We report that 138 miRNAs
are increased on the induction of differentiation. We
compared the entire list of candidate mRNA targets
of up-regulated miRNAs with that of mRNA downregulated
in ESCs on induction of differentiation.
Among the candidate targets emerging from this analysis,
we found three genes, Smarca5, Jarid1b, and Sirt1,
previously demonstrated to be involved in sustaining
the undifferentiated phenotype in ESCs. On this basis,
we first demonstrated that Smarca5 is a direct target of
miR-100, Jarid1b of miR-137, and we also confirmed
previously published data demonstrating that Sirt1 is a
direct target of miR-34a in a different context. The
suppression of these three miRNAs by anti-miRs caused
the block of ESC differentiation induced by LIF withdrawal.
On the other hand, the overexpression of the
three miRNAs resulted in an altered expression of
differentiation markers. These results demonstrate that
miR-34a, miR-100, and miR-137 are required for
proper differentiation of mouse ESCs, and that they
function in part by targeting Sirt1, Smarca5, and
Jarid1b mRNAs.—Tarantino, C., Paolella, G., Cozzuto,
L., Minopoli, G., Pastore, L., Parisi, S., Russo, T.
miRNA 34a, 100, and 137 modulate differentiation of
mouse embryonic stem cells
L' "Itinerarium mentis in Deum " di Ugo Spirito
No full textIl testo tratta dell'itinerario filosofico-religioso di Ugo Spirito, in rapport a Giovanni Gentile e ad altri autori, alla luce del più recente materiale inedit
On cyclic automorphisms of a group
No full textAn endomorphism α of a group G is called a cyclic endomorphism if the subgroup is cyclic for all elements x of G. It can be proved that every cyclic endomorphism is normal, i.e. it commutes with every inner automorphism of G (see [F. de Giovanni,
M. L. Newell and A. Russo, On a class of normal endomorphisms of groups, J. Algebra its Appl. 13 (2014) 6pp.]). In this paper, some further properties of cyclic endomorphisms will be pointed out. Moreover, the structure of a group G in which the group CAut(G)
of cyclic automorphisms has finite index in Aut(G) will be investigated
Corrigendum to ‘Eribulin in combination with bevacizumab as second-line treatment for HER2-negative metastatic breast cancer progressing after first-line therapy with paclitaxel and bevacizumab: a multicenter, phase II, single arm trial (GIM11-BERGI)’: (ESMO Open (2021) 6(2), (S2059702921000089), (10.1016/j.esmoop.2021.100054))
No full textThe authors regret that at the time the article was published the following two authors were missing from the author list: R. Caputo and D. Cianniello. Both authors affiliation is the Breast Oncology Department, Istituto Nazionale Tumori Fondazione G. Pascale, Naples, Italy. The updated author list is as follows: C. De Angelis, D. Bruzzese, A. Bernardo, E. Baldini, L. Leo, A. Fabi, T. Gamucci, P. De Placido, F. Poggio, S. Russo, V. Forestieri, R. Lauria, I. De Santo, R. Caputo, D. Cianniello, A. Michelotti, L. Del Mastro, M. De Laurentiis, M. Giuliano, S. De Placido, G. Arpino. The authors would like to apologise for any inconvenience caused
S-Adenosyl-L-Methionine Overcomes uL3-Mediated Drug Resistance in p53 Deleted Colon Cancer Cells
No full textPurpose: In order to study novel therapeutic approaches taking advantage of natural compounds showing anticancer and anti-proliferative effects, we focused our interest on S-adenosyl-l-methionine, a naturally occurring sulfur-containing nucleoside synthesized from adenosine triphosphate and methionine by methionine adenosyltransferase, and its potential in overcoming drug resistance in colon cancer cells devoid of p53. Results: In the present study, we demonstrated that S-adenosyl-l-methionine overcomes uL3-mediated drug resistance in p53 deleted colon cancer cells. In particular, we demonstrated that S-adenosyl-l-methionine causes cell cycle arrest at the S phase; inhibits autophagy; augments reactive oxygen species; and induces apoptosis in these cancer cells. Conclusions: Results reported in this paper led us to propose S-adenosyl-l-methionine as a potential promising agent for cancer therapy by examining p53 and uL3 profiles in tumors to yield a better clinical outcomes
Synthesis of the trisaccharide component of the repeating unit of the capsular polysaccharide of Streptococcus pneumoniae type 19F
No full textAcetolysis of methyl 4-O-(2-acetamido-3,4,6-tri-O-benzyl-2-deoxy-β-D-mannopyranosyl)-2,3,6-triO-benzyl-α-D-glucopyranoside (3) followed by treatment with hydrogen bromide–acetic acid afforded 4-O-(2-acetamido-6-O-acetyl-3,4-di-O-benzyl-2-deoxy-β-D-mannopyranosyl)-6-O-acetyl-2-O-benzyl-α-D-glucopyranosyl bromide (5). Compound (5) by Hg(CN)2–HgBr2 promoted condensation with benzyl 3,4-di-O-benzyl-α-L-rhamnopyranoside (6) provided the glycosylation product (22% yield) exclusively in the α-configuration.
Hydrogenolysis of the benzyl protecting groups of the obtained trisaccharide and O-deacetylation of the 6′ and 6′′ positions eventually furnished O-(2-acetamido-2-deoxy-β-D-mannopyranosyl)-(1→4)-O-α-D-glucopyranosyl-(1→2)-α,β-L-rhamnopyranose (1), the trisaccharide component of the repeating unit of Streptococcus pneumoniae type 19F
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