1,721,013 research outputs found
SERS active gold nanostructures for selective and ultrabright biolabelling: synthesis and quantitative study
No full textSurface enhanced Raman scattering (SERS) probes based on gold nanoparticles (AuNPs) – organic dyes nanocomposites disclosed new opportunities for multiplexed ultrasensitive biolabelling in vitro or in vivo. SERS labels also have excellent biocompatibility and can be excited with near infrared laser sources, that match the transparency window of biological tissues. Here we report about the preparation of AuNPs obtained by laser ablation synthesis in solution (LASiS)[1,2] and their functionalization with a series of Raman reporter with different spectral fingerprints. The effective differential Raman scattering cross section of these labels were evaluated using liquid Raman standards and the analysis of the surface plasmon band of AuNPs with the Mie – Gans models.[3] We estimated that few (<10) SERS labels are enough to collect a clear Raman spectrum with a common Raman spectrometer. SERS labels were used for the study of nanoparticles uptake in macrophages[4] in order to quantify the number of nanoparticles phagocytized after different times. The conjugation of SERS labels with antibodies that selectively binds antigens over-expressed by specific types of cancerous cells is under investigation for the antibody – directed selective ultrasensitive detection and imaging of cancer.
[1] V. Amendola, M. Meneghetti, Phys. Chem. Chem. Phys. 2009,
[2] V. Amendola, M. Meneghetti; J. Mater. Chem. 2007, 17, 4705–4710.
[3] V. Amendola, M. Meneghetti, J. Phys. Chem. C 2009, 113, 4277–4285.
[4] V. Amendola, M. Meneghetti, S. Fiameni, G. Fracasso, A. Boscaini, M. Colombatti; Submitte
Engineered human nanoferritin for cancer therapy: development and biophysical characterization
Full text linkHuman ferritin H-chain (HFt) nanoparticles represent one of the most appropriate vectors for cellular delivery of molecules thanks to their specific cell internalization by human Transferrin Receptor 1 (CD71), a transmembrane receptor overexpressed in most cancer cell types. In this PhD thesis I report the determination of the structure of HFt and CD71 in the form of complex, by using cryo electron microscopy (cryo-EM) technique. The two proteins have been shown to interact very tightly with nanomolar affinity, as assessed by Surface Plasmon Resonance (SPR) experiments. Our cryo-EM data unveil the residues involved in this contact and provide a sound structural basis to elaborate on the possibility of developing alternative ferritin-like anti-viral or anti-parasite therapeutic ligand, be it an antibody or a peptidomimetic capable of blocking the “common contacts” epitope on CD71 residue, and to further engineering ferritins as theranostic agents.
In this thesis I also report the development and characterization, in vitro and in vivo, of two HFt-based nanovectors containing Mitoxantrone (MIT) or Genz-644282 as cancer drug payload, named HFt-MP-PASE-MIT or The-0504, respectively. The results reported here are very promising and pave the way for an effective cancer-targeted chemotherapy using our HFt-based nanovector platforms
Un modello di simulazione come supporto decisionale alla pianificazione della produzione
No full textGoing Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Progettazione di un Sistema Informativo di Produzione Integrato per Linee di Produzione di Lampade
No full textVariations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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