72 research outputs found
Advanced quantitative proteomics to evaluate molecular effects of low-molecular-weight hyaluronic acid in human dermal fibroblasts
No full textHyaluronic acid (HA) is physiologically synthesized by several human cells types but it is also a widespread ingredient of commercial products, from pharmaceuticals to cosmetics. Despite its extended use, the precise intra- and extra-cellular effects of HA at low-molecular-weight (LWM-HA) are currently unclear. At this regard, the aim of this study is to in-depth identify and quantify proteome's changes in normal human dermal fibroblasts after 24 h treatment with 0.125, 0.25 and 0.50 % LMW-HA (20−50 kDa) respectively, vs controls. To do this, a label-free quantitative proteomic approach based on high-resolution mass spectrometry was used. Overall, 2328 proteins were identified of which 39 significantly altered by 0.125 %, 149 by 0.25 % and 496 by 0.50 % LMW-HA. Protein networking studies indicated that the biological effects involve the enhancement of intracellular activity at all concentrations, as well as the extracellular matrix reorganization, proteoglycans and collagen biosynthesis. Moreover, the cell's wellness was confirmed, although mild inflammatory and immune responses were induced at the highest concentration. The more complete comprehension of intra- and extra-cellular effects of LMW-HA here provided by an advanced analytical approach and protein networking will be useful to further exploit its features and improve current formulations
Appalachian Literature and the Red-Headed Stepchild of Publishing: The Writings of Victor Depta and the Cultural Work of Independent Presses
No full textOver the past couple of decades, Appalachian literature has developed a strong and close relationship with independent publishing, showing the latter to be an important medium for the expression for Appalachian voice. As the attempted consolidation of the book trade into a corporate, bottom-line oriented, high-profit industry minimizes the publication of books with mere regional appeal at the same time that the cultural products of Appalachia, as a region, continue to be marginalized through the continued deployment of stereotypes and attitudes of inferiority, Appalachian writers find it difficult to have their books published and distributed by major publishing houses. As a remedy to this problem, independent publishers are flourishing, and this thesis looks at the work of one prolific author/publisher in particular, Victor Depta, who exemplifies this phenomenon. The published work of Victor Depta crafts a new characterization of Appalachian, by its very defiance of the stereotypes that try to limit that characterization and prevent its voice from being heard
ATMOSPHERIC-PRESSURE IONIZATION MASS-SPECTROMETRIC ANALYSIS OF NEW ANIONIC SURFACTANTS - THE ALKYLPOLYGLUCOSIDE ESTERS
No full textAtmospheric-pressure ionization mass spectrometry has been successfully applied to characterization of a new class of anionic surfactants, the alkylpolyglucoside esters of sulfosuccic, citric and tartaric acid. Complex mixtures of final and intermediate products were injected directly into the ion source without prior chromatographic separation. The constituents were identified on the basis of quasi-molecular ions: cationized ions or solute-solute cluster ions in positive-ion mode, and deprotonated ions in negative-ion mode. The mass-spectrometric data show that all three final products contain one nonionic and two different types of anionic surfactants. The ''real time,'' highly sensitive mass-spectrometric approach proposed here is well suited for quality control testing of tensides, to ensure the safety of the final product, and far the validation of the manufacturing process, because it is able to identify the individual components of the mixture
[Regeneration of endogenous antioxidants, ascorbic acid, alpha tocopherol, by the oligomeric procyanide fraction of Vitus vinifera L.:ESR study]
No full textsj-pdf-1-imr-10.1177_03000605221101970 - Supplemental material for Programmed multi-level ventilation in COVID-19-related acute respiratory distress syndrome: a multi-center retrospective observational study
No full textSupplemental material, sj-pdf-1-imr-10.1177_03000605221101970 for Programmed multi-level ventilation in COVID-19-related acute respiratory distress syndrome: a multi-center retrospective observational study by Filip Depta, Pavol Török, Andrew G. Miller, Peter Firment, Jozef Leškanič, Adam Porubän, Pavol Halaš, Stanislav Mandinec, Vladimír Filka, Henryk Zajac, Michael A. Gentile and Marko Zdravkovic: the DBDS Genomic Consortium in Journal of International Medical Research</p
DEM-Based Approach for the Modeling of Gelation and Its Application to Alginate
No full textThe gelation of biopolymers is of great interest in the material science community and has gained increasing relevance in the past few decades, especially in the context of aerogels-lightweight open nanoporous materials. Understanding the underlying gel structure and influence of process parameters is of great importance to predict material properties such as mechanical strength. In order to improve understanding of the gelation mechanism in aqueous solution, this work presents a novel approach based on the discrete element method for the mesoscale for modeling gelation of hydrogels, similarly to an extremely coarse-grained molecular dynamics (MD) approach. For this, polymer chains are abstracted as dimer units connected by flexible bonds and interactions between units and with the environment, that is, diffusion in implicit water, are described. The model is based on Langevin dynamics and includes an implicit probabilistic ion model to capture the effects of ion availability during ion-mediated gelation. The model components are fully derived and parameterized using literature data and theoretical considerations based on a simplified representation of atomistic processes. The presented model enables investigations of the higher-scale network formation during gelation on the micrometer and millisecond scale, which are beyond classical modeling approaches such as MD. As a model system, calcium-mediated alginate gelation is investigated including the influence of ion concentration, polymer composition, polymer concentration, and molecular weight. The model is verified against numerous literature data as well as own experimental results for the corresponding Ca-alginate hydrogels using nitrogen porosimetry, NMR cryoporometry, and small-angle neutron scattering. The model reproduces both bundle size and pore size distribution in a reasonable agreement with the experiments. Overall, the modeling approach paves the way to physically motivated design of alginate gels
CHARACTERIZATION OF CISPLATIN-GLUTATHIONE ADDUCTS BY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY - EVIDENCE FOR THEIR FORMATION IN-VITRO BUT NOT IN-VIVO AFTER CONCOMITANT ADMINISTRATION OF CISPLATIN AND GLUTATHIONE TO RATS AND CANCER-PATIENTS
No full textAfter incubation of equimolar amounts of cisplatin (CDDP) and glutathione (GSH) in phosphate buffer pH 7.4 at 37 degrees C, we detected two CDDP-GSH adducts whose structures, characterized by LC-MS, corresponded to cis-[Pt(NH3)(2)Cl(SG)] and cis-{[Pt(NH3)(2)Cl](2)(mu-SG)}(+). The latter is a new CDDP-GSH adduct, which was postulated but never structurally characterized so far. Rats and patients were given a 15-min intravenous infusion of CDDP (10 mg/kg to rats and 25 mg/m(2) to patients) preceded by a GSH intravenous administration (200 mg/kg to rats as a bolus and 1.5 g/m(2) to patients as a 15-min infusion). After the administrations, CDDP-GSH adducts were absent in rat and human plasma ultrafiltrates. The discrepancy between in vitro and in vivo findings can be explained based on pharmacokinetic considerations
Wzorzec gatunkowy skargi
No full textIn the article author treats complaint as one of the most important human emotional needs verbalization, as the way of expressing the negative emotions. The aim of the article is to describe some linguistic aspects of complaint understood as a speech genre. The author recognizes complaint as a separate kind of text, composed of sequences of elements. According to the conception of textual pattern she carries out the reconstruction of complaint textual (genre) pattern. She maintains this type of text obligatory contains semantic component: ‘something wrong is happening to me, 1 feel badly about it’. This component contains the negation, which shows speaker’s emotional attitude to his life. The negation, existing in the text, describes “negative” world speaker lives in, but it suggests the better, “positive” possiblities, too. This contrast that is noticed by complaining speaker causes his feeling of discomfort. The author presents semantic definition of the complaint basic structure: [I (X) say: Ifeel badly about Y]. This basic structure can be realized in different ways, creating the structurally different texts. All the elements existing in complaint structure are facultative (exclamations, rethotical questions, oppositions, repetitions, enumerations), but every complaint obligatory contains the negation on its surface or in semantic structure of worlds. However, in spite of their different composition people can recognize the complaints by intuitive identyfication of most important complaint component: speaker’s discontent
MDPI Open Peer Review Corpus 2
No full textMDPI Open Peer Review Corpus 2Section for Logic & Cognitive Science, Institute of Philosophy and Sociology, Polish Academy of ScienceCognitive Metascience LabGenerated by Ksawery Jasieński, with some input from Remigiusz Depta, under supervision of Marcin Miłkowski (2022-2023)---MDPI is committed to open peer review idea, but these are voluntary. They are not available for download in a single package, so they must be crawled from their website.This dataset contains all peer reviews available on mdpi.com as of January 2023, covering over 135 thousand papers. These are in plaintext format (look for TXT files). In addition, the corpus contains metadata in JSON format for particular reviews, author responses, as well as original paper metadata. For reference see the JSON `schema` files available in the GitHub repository associated with this project.Additionally, this dataset contains the source HTML for each website from which the text of reviews was extracted, as well as any supplementary materials attached with the reviews. The original files were not enriched with any linguistic annotation or converted to any format (these are predominantly PDF and DOCX files, as uploaded through the MDPI editorial system by authors and reviewers).We are making source code available for the dedicated crawler that was built to scrape the MDPI database. See the GitHub link below:https://github.com/cognitive-metascience/review_crawler/tree/main/crawlingSee this corpus on PubPeer:https://pubpeer.com/publications/25353AAFD4FC52E2BEC8C7AD08B259#---This dataset is split into parts because of the upload limits of this repository. The archives are available in ZIP (33 parts, look for `.z[01-33]` files) and 7z (23 parts) formats. In addition, we provide the set of excluded articles with incomplete information (e.g., missing some reviews in the first round etc.) in the `mdpi-dump-dir.zip` file.The dataset after unpacking is a little over 170 GB in size.The files are being made available under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).Changelogcurrent version - August 2023no new reviews addedre-scraped (June 2023) the reviews that had some sections of text missingdataset now also includes the source HTML from mdpi.com for each reviewed article. The webpage content was cleaned before storing to an HTML file: specifically, all comments are removed from the document, as well as the following tags: 'script', 'style', 'noscript', 'link', 'rect'.14th March 2023first submission of this datasetreviews was scraped in early January 2023dataset contains metadata for 135652 peer-reviewed articles from the MDPI database, along with full plain text for each review and any supplementary materials that were attached (PDF or DOC files containing e.g. author responses to comments)</p
From waste to valuable cosmetic raw material : the valorization of coffee silverskin following the approach of circular economy
No full textCoffee silverskin (CS) is a tegument of coffee beans that constitutes a by-product of the roasting process. CS has no commercial value and is currently discarded as a solid waste. CS chemical composition can be broken up into cellulose, lignin, proteins, lipids and phenolic compounds, each of which has a great potentiality as a raw material in many fields, included cosmetics. The aim of the work is to selectively extract the lipid and the phenolic fractions from CS and to study their use as functional and active ingredients in cosmetic formulations. This project is part of a bigger program named CirCo (Circular Coffee), focused on the valorization of this residue of agro-food industry, not only in cosmetics, but also for innovative and sustainable solutions in paper production. CirCo involves different academic and industrial research groups and aims at creating a model that embraces the increasingly emerging circular economy approach, practicing industrial symbiosis, waste-to-resource vision and life cycle thinking. CS extracts are obtained using supercritical fluid extraction (SFE), obtaining a promising lipid fraction. The compound is a semisolid brownish material and shows a peculiar composition of fatty acids, with a significant amount of C20:0 and C22:0. The organoleptic and the chemico-physical properties of the resulting lipid fraction are firstly assessed. Its chemical composition is determined by means of fatty acid and unsaponifiable matter analyses. Volatile content analysis, FT-IR, DSC and GPC are used to acquire further technical data. Chemico-physical characterization is targeted on one side to assess the safety and the regulatory compliance, on the other side to classify the lipid fraction in terms of cosmetic properties. A stability study is then performed on the raw material to define its oxidation stability and shelf life, while compatibilities with other ingredients are carried out to guide the formulation. Based on the results obtained, the most suitable formulations are selected to generate prototypal make-up products using the lipid fraction of silverskin. The underway scale-up of the extraction from lab to pilot scale confirms the extraction protocol and guarantees the needed amount of materials for formulating new cosmetic products. The choice to valorize a residue of agro-food industry for different applications, the adoption of a promising and green process such as SFE, the network created among reaserch groups of academia and industry are virtuous elements useful to explore how far we are from a complete sustainable industrial development, where environment, business, quality and safety standards need to fit the same big picture
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