258 research outputs found
Bioremediation and Soil Fertility Study: Effects of Vermiremediation on Soil Contaminated by Chlorpyrifos
No full textfirst_page settings Order Article Reprints Open AccessArticle A Bioremediation and Soil Fertility Study: Effects of Vermiremediation on Soil Contaminated by Chlorpyrifos by Francesca Tagliabue 1,†, Enrica Marini 1,†, Arianna De Bernardi 1,* [ORCID] , Costantino Vischetti 1,* [ORCID] , Gianluca Brunetti 1,2 [ORCID] and Cristiano Casucci 1 [ORCID] 1 Department of Agricultural, Food and Environmental Sciences, Polytechnic University of Marche, 60131 Ancona, Italy 2 Future Industries Institute, University of South Australia, Mawson Lakes Boulevard, Mawson Lakes, SA 5095, Australia * Authors to whom correspondence should be addressed. † These authors contributed equally to this work. Environments 2025, 12(5), 136; https://doi.org/10.3390/environments12050136 Submission received: 27 February 2025 / Revised: 28 March 2025 / Accepted: 20 April 2025 / Published: 24 April 2025 (This article belongs to the Special Issue Editorial Board Members’ Collection Series: Soil Contamination and Remediation) Download keyboard_arrow_down Browse Figures Versions Notes Abstract Although the broad-spectrum pesticide chlorpyrifos (CP) was banned in many developed countries, it is still widely used in developing countries. Its residues persist in the environment for unpredictable times. CP is toxic to various non-target organisms and humans and inhibits soil enzyme activity and bacterial and fungal abundance. This paper aimed to evaluate the effect of vermiremediation on soil chlorpyrifos content and soil fertility. The application of Eisenia fetida or vermicompost was studied in terms of soil chlorpyrifos concentration, microbial biomass content, and enzymatic activities in a 120-day trial. Pesticide application rates were 0, 25, and 50 ppm. The CP did not affect the earthworm survival rate at the tested doses. The earthworms markedly increased microbial biomass carbon and the activity of β-glucosamminidase, while the vermicompost had a noticeably positive effect mainly on alkaline phosphatase activity. Finally, although the vermiremediation techniques studied did not perform a bioremediation activity, they proved effective in improving the biological fertility of the soil in the presence of high concentrations of chlorpyrifos
Hisarlık-Troia: un “luogo di memoria” in età preclassica
No full textA lungo le interpretazioni e le ricostruzioni storiche dell’insediamento di Troia in epoca preclassica hanno enfatizzato l’importanza della migrazione eolica e delle reti commerciali greche. Le recenti indagini archeologiche nell’antica Ilion e nell’Anatolia Occidentale, tuttavia, hanno rivelato un buon grado di continuità culturale nel corso dell’Età del Ferro: i residenti adottarono lentamente elementi comuni a tutto il mondo egeo, integrandoli nel corso del tempo alle consolidate tradizioni locali. Gli scavi effettuati all’interno del settore D9 e nell’area del cosiddetto West Sanctuary, situata nei pressi delle fortificazioni del Bronzo Tardo, hanno evidenziato come alcune attività, verosimilmente volte a rievocare e celebrare un passato glorioso, fossero in realtà già praticate nei primi secoli del I Millennio. Il presente studio, pertanto, mostrerà come probabilmente le narrazioni della Guerra di Troia – confluite poi nell’Iliade e Odissea – non fossero celebrate solamente nel Teatro o nell’Odeon in epoca classica, ma venissero in qualche modo ricordate durante le prime fasi dell’Età del Ferro, facendo di Troia un vero e proprio “luogo di memoria” adatto a creare un forte senso di appartenenza ad una comunità locale
Effects of Tgds knockdown in ATDC5 cells’ chondrogenic differentiation
No full textCatel–Manzke syndrome is a rare autosomal recessive genetic disorder characterized by a symptomatology known as "Pierre-Robin sequence": patients exhibit a small mandible, cleft palate, and a tendency of the tongue to obstruct the airway and digestive tract. Another characteristic is the presence of an additional phalanx affecting the index finger, resulting in finger deviation (clinodactyly). Other malformations have been observed in the cardiac, articular, and other skeletal regions. The syndrome is also associated with growth delay. Catel-Manzke syndrome is related to mutations in the TGDS (dTDP-D-glucose 4,6-dehydratase) gene, located on chromosome 13. The specific function of TGDS in vertebrates is unknown.
We used ATDC5 cells, a well-established model for studying chondrogenesis, to evaluate the effect of TGDS knockdown. ATDC5 TGDS knockdown cells were generated using lentiviral shRNA trasduction (shRNA49). Cells were stimulated with ITS (insulin/transferrin/selenium) for up to 21 days to induce chondrogenesis. Chondrogenic differentiation was assessed by analyzing the expression levels of specific marker genes - SOX9, ACAN, COL2A1, and COL10A1- via quantitative real-time PCR (qPCR).
Quantitative real-time PCR analysis confirmed efficient knockdown of TGDS expression in sh49 cell line compared to the scrambled control (scr). During chondrogenic induction, sh49 knockdown led to a consistent reduction in the expression of key markers, including SOX9, ACAN, COL2A1, and COL10A1, throughout the differentiation time course (days 7, 14, and 21). This indicates an impairment of the chondrogenic process in sh49 cells. As further confirmation of these findings, Alcian Blue staining revealed a marked decrease in proteoglycan production in sh49 cells compared to the scrambled control, consistent with reduced chondrogenic differentiation.
Taken together, these results suggest that TGDS plays a functional role in promoting chondrogenic differentiation
Investigating the role of Tgds in ATDC5 cells’ chondrogenic differentiation
No full textCatel–Manzke syndrome (CMS) is a rare autosomal recessive genetic disorder characterized by a symptomatology known as "Pierre-Robin sequence": patients exhibit a small mandible, cleft palate, and a tendency of the tongue to obstruct the airway and digestive tract. Another characteristic is the presence of an additional phalanx affecting the index finger, resulting in finger deviation (clinodactyly). Other malformations have been observed in the cardiac, articular, and other skeletal regions. CMS is related to mutations in the TGDS (dTDP-D-glucose 4,6-dehydratase) gene, located on chromosome 13. The specific function of TGDS in vertebrates is unknown.
We used ATDC5 cells, a well-established model for studying chondrogenesis, to evaluate the effect of TGDS knockdown. ATDC5 Tgds knockdown cells were generated using lentiviral shRNA trasduction (shRNA49). Cells were stimulated with ITS (insulin/transferrin/selenium) for up to 21 days to induce chondrogenesis. Chondrogenic differentiation was assessed by analyzing the expression levels of specific marker genes - Sox9, Acan, Col2a1, and Col10a1- via RT-qPCR.
RT-qPCR analysis confirmed efficient knockdown of TGDS expression in shRNA49 cell line compared to the control. During chondrogenic induction, shRNA49 knockdown led to a consistent reduction in the expression of key markers - Sox9, Acan, Col2a1, and Col10a1. This indicates an impairment of the chondrogenic process in shRNA49 cells. As further confirmation of these findings, Alcian blue staining revealed a marked decrease in proteoglycan production in shRNA49 cells compared to the scrambled control, consistent with reduced chondrogenic differentiation.
Taken together, these results suggest that TGDS plays a functional role in promoting chondrogenic differentiation
IL PONTE E IL TERRITORIO
No full textRaccolta di proposte progettuali sviluppate nell'ambito di tesi di Laurea discusse presso l'Istituto Universitario di Architettura di Venezia
Usefulness of nutraceuticals in migraine prophylaxis
No full textSeveral studies have supported the efficacy of complementary and alternative medicine approaches (physical, behavioral and nutraceutical therapies) in the treatment of headache disorders. Nutraceutical treatment consists of taking vitamins, supplements (magnesium, riboflavin, coenzyme Q10, and alpha lipoic acid) and herbal preparations (feverfew and butterbur), and its usage is frequently determined by dissatisfaction with conventional medical therapies. There is a growing body of research on nutraceutical use for migraine prophylaxis. This brief overview provides information about the potential efficacy and side effects of various nutraceutical products summarizing randomized controlled trials of some of the most commonly used non-pharmacological treatments for the prophylaxis and treatment of migraine, including magnesium, coenzyme Q10, riboflavin (vitamin B2), petasites, and feverfew
Il monitoraggio della barbabietola da zucchero (Beta vulgaris L.) mediante l'analisi della riflettanza spettrale.
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