1,721,071 research outputs found
Memoria difensiva in tema di competenza per territorio per il delitto di aggiotaggio su strumenti finanziari
No full text
High throughput tools for the study of protein-nanostructured surface interaction
No full textThe aim of this review is to describe and to analyze the ingredients that are necessary in order to develop a robust and effective experimental approach for the high throughput characterization of protein-nanostructured surface interaction. In the first part of this paper we review the nanostructured surface synthesis methods that are potentially able to create nanostructured inorganic surface libraries. In the second part, we address another fundamental aspect consisting in the availability of high throughput proteins detection methods. We describe in details new emerging analytical tools compatible with nanostructured surfaces, analyzing different possible strategies, depending on the objective of the experiment and on the library format
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
An introduction to the 4th edition of the International Workshop on Functional and Operatorial Statistics
No full textLinz winter school and workshop
No full textCluster-assembled nanostructured Titanium Oxide (ns-TiOx) deposited by Supersonic Cluster Beam Deposition (SCBD)recently proved to be a very promising biomaterial, allowing the adhesion and proliferation of cancer and primary cells, with no need of additional coating with extra-cellular matrix proteins, and the adhesion of proteins, such as streptavidin, with no need of additional coatings of polycations. The intrinsic nanostructure of this material, with fine granularity, high porosity and specific area, coupled to the chemical reactivity of the surface is likely a key element in determining the biological affinity of the material with nanometer-sized biomolecules, such as proteins.[4] Little is known however of the specific role played by each of these surface properties in the interaction of proteins with nanostructured biocompatible materials.
Here we present an atomic force microscopy (AFM) study of the morphological and adhesive properties of ns-TiOx biocompatible surfaces. AFM provides nanometer spatial resolution in both imaging and force spectroscopy, and it is therefore the technique of choice for the investigation of biologically relevant surface properties of materials.
AFM in force-volume mode (FV-mode) has been used to characterize the local adhesive properties of ns-TiOx, in an effort to establish a correlation between the macroscopic wetting behavior of ns-TiOx films and those local morphological and chemical properties, which can be relevant in protein adhesion processes. We have developed a patterning strategy based on the combined use of SCBD and nanosphere lithography, for the production of sub-micrometer patterns of ns-TiOx on glass or other reference substrates (Fig.1). The main objective of developing such patterns is to have reference and target materials simultaneously in the same experiment. In FV-mode, we have studied the interaction of silicon nitride (Si3N4) tips with ns-TiOx and glass, as reference. The results obtained in aqueous medium suggest that ns-TiOx interacts with Si3N4 via chelation mechanisms, in addition to hydrogen bonding: these mechanisms are responsible for stronger adhesion of the AFM tip with ns-TiOx surface as compared to the reference glass substrate. The presence of reference during the investigation of the target material helped us resolving ambiguities that show up when reference and target material are investigated independently. Local adhesion measurements in aqueous media allowed us to get insights on basic interaction mechanisms between surface groups that are expected to play a major role in the interactions between proteins and biocompatible surfaces
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
An AFM investigation of the interfacial properties of biocompatible nanostructured titanium oxide films
No full textCluster-assembled nanostructured Titanium Oxide (ns-TiOx) deposited by Supersonic Cluster Beam Deposition (SCBD) [1] recently proved to be a very promising biomaterial, allowing the adhesion and proliferation of cancer and primary cells, with no need of additional coating with extra-cellular matrix proteins [2], and the adhesion of proteins, such as streptavidin, with no need of additional coatings of polycations [3]. The intrinsic nanostructure of this material, with fine granularity, high porosity and specific area, coupled to the chemical reactivity of the surface is likely a key element in determining the biological affinity of the material with nanometer-sized biomolecules, such as proteins.[4] Little is known however of the specific role played by each of these surface properties in the interaction of proteins with nanostructured biocompatible materials.
Here we present an atomic force microscopy (AFM) study of the morphological and adhesive properties of ns-TiOx biocompatible surfaces. AFM provides nanometer spatial resolution in both imaging and force spectroscopy, and it is therefore the technique of choice for the investigation of biologically relevant surface properties of materials.
AFM in force-volume mode (FV-mode) has been used to characterize the local adhesive properties of ns-TiOx, in an effort to establish a correlation between the macroscopic wetting behavior of ns-TiOx films and those local morphological and chemical properties, which can be relevant in protein adhesion processes. We have developed a patterning strategy based on the combined use of SCBD and nanosphere lithography, for the production of sub-micrometer patterns of ns-TiOx on glass or other reference substrates (Fig.1). The main objective of developing such patterns is to have reference and target materials simultaneously in the same experiment. In FV-mode, we have studied the interaction of silicon nitride (Si3N4) tips with ns-TiOx and glass, as reference. The results obtained in aqueous medium suggest that ns-TiOx interacts with Si3N4 via chelation mechanisms, in addition to hydrogen bonding: these mechanisms are responsible for stronger adhesion of the AFM tip with ns-TiOx surface as compared to the reference glass substrate. The presence of reference during the investigation of the target material helped us resolving ambiguities that show up when reference and target material are investigated independently. Local adhesion measurements in aqueous media allowed us to get insights on basic interaction mechanisms between surface groups that are expected to play a major role in the interactions between proteins and biocompatible surfaces
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