1,267 research outputs found
NMR and computational data of two novel antimicrobial peptides
No full textAbstractHere we report details on the design and conformational analysis of two novel peptides showing antimicrobial properties, as reported in the research article, “New antimicrobial peptides against foodborne pathogens: from in silico design to experimental evidence” G. Palmieri, M. Balestrieri, Y.T.R. Proroga, L. Falcigno, A. Facchiano, A. Riccio, F. Capuano, R. Marrone, G. Campanile, A. Anastasio (2016) [1]. NMR data, such as chemical shifts in two different solvents as well as aCH protons deviations from random coil values and NOE patterns, are shown together with the statistics of structural calculations. Strategy and particulars of molecular design are presented
Middle Miocene out-of sequence thrusting and successive exhumation in the Peloritani Mountains, Sicily: Late stage evolution of an orogen unreveled by apatite fission track and (U-Th)/He thermochronometry
No full textApatite fission track (AFT) and (U‐Th)/He (AHe)
thermochronometry are applied to constraint the thermal
history of the basement rock forming the Peloritani
Mountains in northeast Sicily, Italy. AFT ages
range between 29.0 ± 5.5 Ma and 5.5 ± 0.9 Ma while
AHe ages vary from 19.4 Ma to 3.3 Ma. Most AFT
ages are younger than the overlying terrigenous
sequence that, in turn, postdates the main orogenic
phase. Through the coupling of the thermal modeling
with the stratigraphic record, a middle Miocene thermal
event is revealed. This event affected an innerintermediate
portion of the Peloritani Mountains and
is confined by the distribution of the AFT ages <15 Ma. The U‐shaped pattern of AFT ages along N–S transects
across the mountain chain is consistent with burial
below a thrust stack that is a few km thick, created by
an out‐of‐sequence thrusting phase that affected the
inner portion of the belt. The tectonic load was then
removed initially by erosion enhanced by the high
relief at 10–7 Ma. The difference between young
AFT and AHe ages is used to infer a rate of exhumation
of 0.3 mm/yr for this stage. The gap between the
youngest AHe ages and the sedimentary record above
indicate a final exhumation stage with increasing rates
of denudation (1–3 mm/yr) since the Pliocene times
due to extensional tectonics. Citation: Olivetti, V., M. L.
Balestrieri, C. Faccenna, F. M. Stuart, and G. Vignaroli (2010),
Middle Miocene out‐of‐sequence thrusting and successive
exhumation in the Peloritani Mountains, Sicily: Late stage
evolution of an orogen unraveled by apatite fission track and
(U‐Th)/He thermochronometry
SIRT3 and metabolic reprogramming mediate the antiproliferative effects of whey in human colon cancer cells
No full textEmerging strategies to improve healthy aging include dietary interventions as a tool to promote health benefits and reduce the incidence of aging-related comorbidities. The health benefits of milk are also linked to its richness in betaines and short-chain acylcarnitines, which act synergisti-cally in conferring anticancer, anti-inflammatory, and antioxidant properties. Whey, despite being a dairy by-product, still has a considerable content of bioactive betaines and acylcarnitines. Here, we investigated the anticancer properties of whey from Mediterranean water buffalo (Bubalus bubalis) milk by testing its antiproliferative effects in colorectal cancer (CRC) cells HT-29, HCT 116, LoVo and SW480. Results indicated that treatment with whey for 72 h inhibited cell proliferation (p < 0.001), induced cell cycle arrest, and apoptosis via caspase-3 activation, and modulated cell metabolism by limiting glucose uptake and interfering with mitochondrial energy metabolism with the highest effects observed in HT-29 and HCT 116 cells. At molecular level, these effects were accompanied by upregulation of sirtuin 3 (SIRT3) (p < 0.01) and peroxisome proliferator-activated receptor (PPAR)-γ expression (p < 0.001), and downregulation of lactate dehydrogenase A (LDHA) (p < 0.01), sterol regulatory-element binding protein 1 (SREBP1) (p < 0.05), and PPAR-α (p < 0.01). Transient SIRT3 gene silencing blocked the effects of whey on the LDHA, PPAR-γ, and PPAR-α protein expressions (p < 0.01) suggesting that the whey capacity of perturbating the metabolic homeostasis in CRC cell lines is mediated by SIRT3
A new system of authorship best assessment
No full textPurpose:The standard bibliometric indexes ("m-quotient "H-," "H2-," "g-," "a-," "m-," and "r-" index) do not considered the research' position in the author list of the paper. We proposed a new methodology, System of Authorship Best Assessment (SABA), to characterize the scientific output based on authors' position. Material and Methods:Four classes S1A, S1B, S2A, and S2B include only papers where the researcher is in first, first/last, first/second/last, and first/second/second-last/last position respectively were used for the calculation of H-index and number of citations The system was tested with Noble prize winners controlled with researchers matched for H-index. The different in percentage between standard bibliometric index and S2B was calculated and compared. Results:The percentage differences in Noble prize winners between S2B-H-index versus Global H-index and number of citations is very lower comparing with control group (median 4.15% [adjusted 95% CI, 2.54-5.30] vs 9.00 [adjusted 95% CI, 7.16-11.84], p < 0.001; average difference 8.7% vs 20.3%). All different in percentage between standard bibliometric index and S2B except two (H2- and m-index) were significantly lower among Noble prize compared with control group. Conclusion:The SABA methodology better weight the research impact by showing that for excellent profiles the S2B is similar to global values whereas for other researchers there is a significant difference
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