35 research outputs found
Assessment of severity of asthma in general practice
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Stabilization of bacterial cells culture on immobilized Alginate beads and optimization of Congo red decolorization
No full text544-552The paper deals with the primary objective to optimize different parameters such as concentration, temperature, pH, time and size of cells for the decolorization of azo dye (Congo red) by using bacterial isolates by the method of immobilized bacterial cells. Staphylococcus aureus immobilized beads show a maximum dye decolorization of 94% under optimum condition and found to be more efficient in dye decolorization. The optimum value for degradation is found to be 35°C, for 400 mg/L of concentration of dye at the medium pH of 7. The order of the reaction was found to be first order with the rate constant of 0.073 h-1. The dye adsorbed on the bead surface follows Freundlich adsorption isotherm with the constant values are K = 1.368 and 1/n = 0.773. Studies have been carried out by FTIR, GC-MS analysis for confirming the biodegradation of Congo Red by the immobilized bacterial cells. The results reported warrant further investigation to establish the usefulness of these isolates for bioremediation and biodegradation application such as wastewater treatment
OPTIMIZATION OF PROCESS PARAMETERS USING RESPONSE SURFACE METHODOLOGY FOR REMOVAL OF PHENOL BY NANO ZERO VALENT IRON IMPREGNATED CASHEW NUT SHELL
No full textObjective: The present research is an attempt to optimize the process parameters for phenol removal from aqueous solution using Nano zero-valent iron (NZVI) impregnated cashew nut shell (NZVI-CNS) by adopting statistical tool Response Surface Methodology-Box Behnken Design (RSM-BB).Methods: Box Behnken Design (RSM-BB) design was used to explore the effect of variables on the removal of phenol. In RSM-BB method, high and low values were assigned for the five variables viz. initial pH, NZVI-CNS dosage, initial concentration, contact time and temperature. The preparation was carried out by simple liquid-phase reduction method, namely borohydride reduction method.Results: The results of RSM-BB method showed the significant effect of pH (A), Dose (B), initial concentration (C), time (D), and temperature (E) on phenol removal from aqueous solution. The results of ANOVA and regression of the second order model showed that the linear effects of Dose (B) and Temperature and cross products effects of temperature and pH were more significant. All the critical variables having the greatest effect on the removal of phenol from Nano zero valent iron impregnated cashew nut shell.Conclusion: Nano zero valent iron impregnated cashew nut shell successfully employed to remove phenol from aqueous solution. The factors optimized in the present work would helpful in phenol removal from aqueous solution.Â
Nickel Silicide Contact for Copper Plated Silicon Solar Cells
No full textabstract: Nickel-Copper metallization for silicon solar cells offers a cost effective alternative to
traditional screen printed silver paste technology. The main objective of this work is to
study the formation of nickel silicide contacts with and without native silicon dioxide SiO2.
The effect of native SiO2 on the silicide formation has been studied using Raman
spectroscopy, Rutherford backscattering spectrometry and sheet resistance
measurements which shows that SiO
2
acts as a diffusion barrier for silicidation at low
temperatures of 350°C. At 400°C the presence of SiO2 results in the increased formation
of nickel mono-silicide phase with reduced thickness when compared to samples without
any native oxide. Pre and post-anneal measurements of Suns Voc, photoluminescence and
Illuminated lock in thermography show effect of annealing on electrical characteristics of
the device. The presence of native oxide is found to prevent degradation of the solar cells
when compared to cells without any native oxide. A process flow for fabricating silicon
solar cells using light induced plating of nickel and copper with and without native oxide
(SiO2) has been developed and cell results for devices fabricated on 156mm wafers have
been discussed.Dissertation/ThesisMasters Thesis Materials Science and Engineering 201
EVALUATION OF MICROBIOLOGICAL TRAITS, ANTIBIOTIC RESIDUE AND FORMALDEHYDE RESIDUE BY TRACKING SEASONAL VARIATIONS IN A SELECTED FISH SPECIES COLLECTED FROM DIFFERENT FISH MARKETS IN PUNJAB, INDIA
No full textThe study aimed to assess microbiological parameters, antibiotic concentrations, and formaldehyde residues in four fish species - Wallago attu, Labeo rohita, Pangasianodon hypophthalmus, and Rastrelliger kanagurta - sourced from both inland and marine sectors, encompassing culture and capture fisheries. These fish were collected from the Amritsar, Jalandhar, Ludhiana, Mohali, and Bathinda fish markets in Punjab, India, over four distinct seasons (post-monsoon, winter, pre-monsoon, and monsoon) from September 2021 to August 2022. The study focused on evaluating Total Plate Count (TPC), Escherichia coli, and Staphylococcus aureus. Additionally, it investigated antibiotic residues and formaldehyde presence to ensure consumer safety. TPC levels in various fish species ranged from 1.193.22×104 cfu/g across different seasons and markets. Elevated levels of E. coli (2.46 - 4.46×10² cfu/g) and S. aureus (2.33 - 4.73×10³ cfu/g), exceeding permissible limits, presented a notable public health concern. The monsoon season recorded the highest microbiological counts in all fish species sampled. Bathinda's fish market had higher average microbiological values compared to the other markets, potentially due to factors like ice quality, hygiene standards during packaging, and storage and transportation processes. In terms of antibiotic residues, the concentrations of chloramphenicol (0.001 - 0.019 µg/kg), nitrofurantoin (0.001 - 0.026 µg/kg), and oxytetracycline (0.001 - 0.028 µg/kg) in the selected species were found to be below permissible limits, ensuring the fish's safety for consumption. No formaldehyde residues were detected in any of the fish species across all seasons and markets, further confirming their safety. Addressing bacterial contamination through scientific interventions remains essential for public health and environmental sustainability
Statistical Evaluation of Medium Components by Plackett-Burman method for Laccase from <i>Pseudomonas aeruginosa</i> ADN04 using Submerged Fermentation
No full textHeterosis studies for quantitative traits in interspecific hybrids of cotton (Gossypium hirsutum L. × Gossypium barbadense L.)
No full text<p>A study was conducted in interspecific cotton hybrids (<em>Gossypium hirsutum</em> L. × <em>Gossypium barbadense</em> L.) to assess the extent of the heterosis for eight yield and yield attributing traits. Twelve lines of <em>G. hirsutum</em> and five testers of <em>G. barbadense</em> were crossed in line x tester manner to develop 60 F<sub>1</sub> hybrids. Analysis of variance revealed the significant differences among the crosses for all traits. Heterosis was worked out over mid parent, better parent and two standard checks i.e. MRC 7918 and Varalaxmi. Crosses RAH1065 x SUJALA, PH1075 x SUJALA, PH348 x SUJALA and DHY286 x Suvin were the best heterotic combinations for seed cotton yield and could be utilized for hybrid development. These hybrids were shown desirable heterosis for seed cotton yield along with other yield components. These hybrids may be tested in large scale trial to confirm the superiority in heterosis.</p></jats:p
Phylogenetic Analysis of The Internal Transcribed Spacer (ITS) Region in Laccase Producing Pseudomonas aeruginosa ADN04 by Predicted rRNA Secondary Structure
No full textSecondary structure and phylogenetic efficacy of the internal transcribed spacer-1 (ITS1) in laccase producing organism were studied among 35 species through molecular phylogenetic analyses. The sequence of laccase gene were taken from the NCBI databases and compared with the identified sequence that was coded for laccase. Similar sequences of laccase synthesizing organisms were downloaded from the database and multiple sequence alignment was performed. The most closely related sequences were taken from each organism and RNA structure was predicted. This structure was used for comparative approach of rRNA sequences to be similar for the aligned sequence. Then the phylogenetic analysis was carried for the above sequences and the results were compared with the RNA structure for comparative analysis. During the evolution, the laccase genes from the prokaryotes were differentiated to the higher organisms was reported
