1,723,434 research outputs found
Accurate delineation of cell cycle phase transitions in living cells with PIP-FUCCI
Full text linkCell cycle phase transitions are tightly orchestrated to ensure efficient cell cycle progression and genome stability. Interrogating these transitions is important for understanding both normal and pathological cell proliferation. By quantifying the dynamics of the popular FUCCI reporters relative to the transitions into and out of S phase, we found that their dynamics are substantially and variably offset from true S phase boundaries. To enhance detection of phase transitions, we generated a new reporter whose oscillations are directly coupled to DNA replication and combined it with the FUCCI APC/C reporter to create “PIP-FUCCI”. The PIP degron fusion protein precisely marks the G1/S and S/G2 transitions; shows a rapid decrease in signal in response to large doses of DNA damage only during G1; and distinguishes cell type-specific and DNA damage source-dependent arrest phenotypes. We provide guidance to investigators in selecting appropriate fluorescent cell cycle reporters and new analysis strategies for delineating cell cycle transitions.</p
Letter dated 26 November 1980 from Maria Fucci to Lorenzo A. Richards
No full textLetter dated 26 November 1980 from Maria Fucci for the Office of the Treasurer of Sigma Xi, the scientific research society, to Lorenzo A. Richards regarding emeritus statusSIGMA XI, THE SCIENTIFIC RESEARCH SOCIETY OFFICE OF THE TREASURER 345 WHITNEY AVENUE NEW HAVEN, CONNECTICUT 06511 (203) 624-9883 26 November 1980 Dr. Lorenzo A. Richards 4455 Fifth Street Riverside, CA 92501 Dear Dr. Richards: This is in reply to your request for information relative to Emeritus membership. The Constitution and Bylaws of the Society defines the conditions necessary to qualify for Emeritus status as follows: "Any active Member or active Associate Member who has paid dues to the Society for the immediately preceding ten years and has reached the age of 65, or who, at any age, has^paid dues to the Society for the immediately preceding 3(V years, may upon retirement from his active association with his educational, research, industrial, or other professional employment, request Emeritus status in the Society." The annual fee for each Emeritus Member or Emeritus Associate Member shall be equal to one-half the current non-member subscription rate to American Scientist. A check of our records here at National Headquarters indicates that you are not eligible for Emeritus status at this time, in that you have not paid dues to the Society for the immediately preceding ten years. If our records are in error, please do not hesitate to contact us. Sincerely yours, ? f 7L 4 - *> D Maria Fucci Office of the Treasurer MF:cav P.S. We have altered our records to reflect your inactive membership, You will receive no further billing notices. ^ v > v ^ ^ ^ ^ ^ ^ ^ iuss<Jf *2_, sK^ it A
Generation of human beta Fucci cell lines.
No full text<p>Two different EndoC-βH2 Fucci derivatives were generated. (A) In one EndoC-βH2 Fucci cell line, the two genes encoding the Fucci indicators (GF: green Fucci, mAG-ΔGeminin and OF: Orange Fucci, mKO2-ΔCdt1) were stably transferred by sequential retroviral transduction using two bicistronic vectors. They encode either GF plus the gene encoding the resistance to zeocin (ZeoR), or OF plus the gene encoding the resistance to puromycin (PuroR). After each step, a selection using the appropriate drug was achieved and the resulting cells line, termed EndoC-βH2-OFP-GFZ, was routinely cultured in presence of both selective drugs. (B) In the other EndoC-βH2 Fucci cell line, the two genes encoding the Fucci indicators (GF: green Fucci, eGFP-ΔGeminin and OF: Orange Fucci, mKO2-ΔCdt1) were present on the same retrovector and separated by a 2A peptide together with the gene encoding the resistance to puromycin. They were thus simultaneously transferred and the resulting cell line, termed EndoC-βH2-PGF2AOF, was selected and routinely cultured in a puromycin-containing medium.</p
FUCCI U2OS cells
No full textFUCCI U2OS cells originally published here. Data can be downloaded and preprocessed into provided H5AD file from here.</p
Un diritto nel ‘labirinto’: la salute tra livelli essenziali di assistenza e costi standard
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FUCCI RPE1 cells
No full textFUCCI RPE2 cells originally published here. Data can be downloaded and preprocessed into provided H5AD file from GEO here with accession number GSE128365.</p
I bambini e la salute: il diritto alla salute come "dititto partecipato"
No full textCompetenze infantili, agency e partecipazione nei percorsi di malattia dei bambin
HeLa FUCCI ANALYSIS
No full textAnalysis of cell cycle transition from G1 to S using Fucci vectors in HeLa cells as well as analysis of mean Mitosis length in the cell typ
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