1,721,000 research outputs found
Produzione di fenoli, antociani e attività dell’enzima fenilalanina ammonio-liasi (PAL) in calli di fragola (Fragaria x ananassa Duch.) coltivati in vitro.
No full textDifferent size, shape and growth behaviour of cells in suspension cultures of strawberry (Fragaria x ananassa Duch.).
No full textNuclear DNA content of runner, regenerated and micropropagated plants of Fragaria x ananassa Duch.
No full textComparative analysis of DNA nuclear content by flow cytometry on strawberry plants propagated via runners and regenerated from meristem and callus cultures.
No full textCryopreservation of Italian cultivars of hazelnut
No full textThe present study was performed to optimise the cryopreservation protocol in Italian cultivars of Corylus avellana L. Lateral buds excised from in vitro grown plantlets of ‘Tonda Gentile Romana’ (TGR) and ‘Montebello’ (MB) were cryopreserved by the encapsulation-dehydration technique. The effect of dehydration of beads with sucrose (0.5, 0.75 or 1 M) for 1 or 3 days, of desiccation with silica gel for 6 or 8 h and of application of two cytokinins (N6-benzyladenine or meta-Topolin) in the regrowth phase was evaluated. The highest regrowth was obtained dehydrating alginate beads with 0.75 M sucrose applied for 1 day and with desiccation for 8 h with silica gel in both cultivars. On the other hand, the regrowth response to the type of cytokinins in the recovery medium was different in the two cultivars: in TGR the highest regrowth, 40% with N6-benzyladenine and 47% with meta-Topolin, was reached without significant differences between the two cytokinins, while in MB meta-Topolin induced significant higher regrowth (40%) than N6-benzyladenine
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Somaclonal variation and flow cytometry in strawberry (Fragaria x ananassa Duch.): how to measure it?.
No full textVariations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Applicazione di metiljasmonato in colture di callo di mirtillo per indurre la produzione di composti fenolici
No full textLo studio dei metaboliti secondari ha registrato un crescente interesse negli ultimi anni, in quanto essi
rappresentano un’importante fonte di molecole con attività biologica; ad esempio, alla base di molti
farmaci ci sono composti naturali chimicamente modificati. L’interesse per i prodotti bioattivi di origine
vegetale ha favorito l’identificazione di numerosi composti con potenziali attività farmacologiche.
Recentemente, sono state notevolmente implementate le tecnologie di produzione di metaboliti secondari
basate sulle colture in vitro (Lila, 2004; Ruffoni et al., 2009a e referenze incluse). I vantaggi
offerti dalle colture in vitro riguardano principalmente la produzione di elevate quantità di materiale
ottenuto da uno stesso espianto, la garanzia di protezione dall’attacco di funghi e batteri attraverso lo
svolgimento delle operazioni in ambienti controllati, la ripetibilità di tale processo e la possibilità di
automatizzarlo. Tuttavia, numerosi aspetti sono ancora da studiare e da chiarire sia a livello tecnologico
che biologico. Molti lavori sono presenti in bibliografia riguardanti l’incremento della produzione di
molecole bioattive in piante coltivate in pieno campo o in ambiente protetto, mentre gli studi su piante
o cellule coltivate in vitro sono limitati e spesso riguardano una sola classe di metaboliti. Le difficoltà
nascono dalla definizione delle condizioni colturali più idonee per la crescita delle colture e dalla
messa a punto di appropriati trattamenti di elicitazione abiotica per incrementare la produzione di
molecole bioattive.
Qui descriviamo un protocollo per indurre produzione di composti fenolici, in particolare antociani e
acido ellagico, in colture di callo di mirtillo americano
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