1,859,995 research outputs found
Digital twin framework for monitoring, controlling and diagnosis of photovoltaic DC microgrids
No full textStatistical Optimization of Green Fluorescent Protein Production from Escherichia coli BL21(DE3)
No full textAn optimized cultivation condition is needed to maximize the functional green fluorescent protein (GFP) production. Six process variables (agitation rate, temperature, initial medium pH, concentration of inducer, time of induction, and inoculum density) were screened using the fractional factorial design. Three variables (agitation rate, temperature, and time of induction) exerted significant effects on functional GFP production in E. coli shake flask cultivation and were optimized subsequently using the Box-Behnken design. An agitation rate of 206 rpm at 31°C and induction of the protein expression when the cell density (OD(600nm)) reaches 1.04 could enhance the yield of functional GFP production from 0.025 g/L to 0.241 g/L, which is about ninefold higher than the unoptimized conditions. Unoptimized cultivation conditions resulted in protein aggregation and hence reduced the quantity of functional GFP. The model and regression equation based on the shake flask cultivation could be applied to a 2-L bioreactor for maximum functional GFP production
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
The Tempo Giusto: A call for recalibrating time in human movement research
No full textHuman movement science now runs on accelerating metrics, short grant cycles, and compressed editorial timelines. Speed can deliver rapid outputs but often erodes validity, reproducibility, and conceptual innovation. We advance time-attuned science – tempo giusto – as a design-and-governance concept that calibrates the pace of inquiry to the intrinsic timescales of human movement. Rather than universal deceleration, it prescribes fast evidence when mechanisms and decision horizons warrant it, and sustained observation with planned replication when effects unfold slowly. Delivering this alignment requires systems that reward durability, openness, and societal relevance over volume or venue prestige. A commitment to temporal justice ensures that access to the time required for valid inquiry is widely shared. Where time, method, and purpose converge, findings become cumulative, credible, and enduring.FCT—Fundação para a Ciência e a Tecnologia—Foundation for Science and Technology, I.P. (Portugal), within the scope of SPRINT - Sport Physical Activity and Health Research & Innovation Center UID/6185/2023
Fatigue assessment of a railway bridge detail using dynamic analysis and probabilistic fracture mechanics
Full text linkThis paper presents a generic methodology for the use of PFM within the context of bridge loading for the fatigue design and assessment of steel railway bridges and provides detailed guidance on how to use the proposed methodology in order to carry out a PFM-based fatigue assessment. The problem is set in a probabilistic context to take into account material, loading as well as modeling uncertainties. Guidance is given on how to calibrate a constant amplitude PFM analysis against an S-N curve. Finally, as a case study, a cracked welded bridge detail is considered and its time-dependent fatigue reliability is established © 2012 Taylor & Francis Group
Environmental communication for expert audiences - experimenting three approaches
No full text| openaire: EC/H2020/819202/EU//SOS.aquaterra Publisher Copyright: © 2023 The Author(s). Published with license by Taylor & Francis Group, LLC.We studied three novel approaches in environmental science communication for experts: gamification, virtual reality, and art-based scenario workshops and analyze participants’ perceptions through qualitative interviews and a survey. Four dimensions emerged from the interviews: enjoyment, usability, sociability, and learning that were found to be important for scientific communication. The approaches were perceived as enjoyable and beneficial for creating dialogue. However, the simplification of the information reduced its usability for experts. The approaches were found suitable for understanding other participants’ viewpoints rather than disseminating knowledge about the content. Experts as a target group require special focus in the development of science communication.Peer reviewe
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
France and the Crusades in the Later Middle Ages
No full textCrusades featured prominently in global geopolitics between the eleventh and seventeenth centurie
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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