91 research outputs found
A coculture-based approach for screening campaigns aimed at idenifying novel Pseudomonas aeruginosa quorum sensing inhibitors
No full textQuorum sensing (QS) is recognized as a promising target for the identification of anti-virulence drugs hampering Pseudomonas aeruginosa adaptability to the host environment and pathogenicity. Consequently, a number of studies in the last decade focused on the identification of small molecules or proteins with anti-QS activity, mainly targeting the las QS system, which is based on N-3-oxododecanoyl-homoserine lactone (3OC12-HSL) as signal molecule. Different experimental approaches have been successfully used to identify QS blockers interfering with the activity/stability of the 3OC12-HSL receptor LasR, with the functionality of the 3OC12-HSL synthase LasI, or with the stability/bioavailability of the 3OC12-HSL signal molecule itself. Here we describe the use of a high-throughput screening system for the identification of novel las QS inhibitors based on the cocultivation of P. aeruginosa wild type and the P. aeruginosa-derived biosensor strain PA14-R3, in which light emission relies on the ability of the wild type strain to synthesize 3OC12-HSL and of the biosensor strain to perceive this signal molecule. With respect to other screening systems, this method has the advantage of being cost-effective and allowing the identification of compounds targeting, besides 3OC12-HSL reception, any cellular process critical for the functionality of the las QS system, including 3OC12-HSL synthesis and secretion
Digital narrative reporting and legitimation tactics in Dieselgate time(s)
No full textThanks to digital technologies, companies can communicate in real-time with their stakeholders through corporate websites and social media profiles. These communication channels may especially be useful when companies incur in scandals and their licence to operate is threatened. In such cases, companies are expected to disclose about the scandal by adopting legitimation tactics. Focusing on the recent dieselgate scandal, this chapter aims at exploring the legitimation tactics employed in digital narratives reported by Volkswagen, Daimler and BMW. A close reading of press releases, Twitter posts, annual reports and sustainability reports published in the aftermath of the scandal is performed to detect the presence of legitimation tactics. Unsurprisingly, Volkswagen is found to adopt a reactive approach when reporting about the dieselgate, diminishing the issue and reassuring its shareholders. Hit by the scandal two years later, Daimler and BMW demonstrate to have learned the lesson by Volkswagen with more sophisticated tactics, but two different attitudes. While Daimler is proactive in conveying detailed measures to the benefit of its customers, BMW is more defensive and downplays the scandal. With its findings, the chapter advances knowledge on the role of digital narratives as legitimizing tools in times of scandals, thereby advising stakeholders about the techniques that companies employ under extreme circumstances
BIOSCOPIUM - Guidelines for mapping urban biodiversity in urban regeneration areas
No full textToday’s challenges related to climate change and biodiversity loss in urban spaces should be tackled as a local, regional, and global emergency by designing with Nature. According to the European Biodiversity Strategy for 2030, cities are called on to lead the conservation and regeneration of biodiversity and to implement it in urban open spaces. Identifying the environmental, cultural, and social values and potentials of urban biodiversity through visuals and maps is part of LAND and Polifactory mission and was specifically explored within T-Factor – Horizon 2020 project in the applicative research “Bioscopium: Guidelines for mapping urban biodiversity in urban regeneration areas”, developed in collaboration with ecological and wildlife experts of Studio TerraViva.
Bioscopium was conceived as a handbook for tackling biodiversity loss in meanwhile spaces and big-scale urban regeneration areas, supporting practitioners, real estate developers, policymakers, and decisionmakers in understanding, preserving, and increasing urban biodiversity while highlighting its crucial benefits for environmental quality and human well-being
Nuove prospettive per il trattamento topico della psoriasi: estratti vegetali ad attivita' anti-TNFalfa
No full textWidespread Multidrug Resistance of Arcobacter butzleri Isolated from Clinical and Food Sources in Central Italy
No full text: The Arcobacter genus comprises a group of bacteria widely distributed in different habitats that can be spread throughout the food chain. Fluoroquinolones and aminoglycosides represent the most common antimicrobial agents used for the treatment of Arcobacter infections. However, the increasing trend of the antimicrobial resistance of this pathogen leads to treatment failures. Moreover, the test implementation and interpretation are hindered by the lack of reference protocols and standard interpretive criteria. The purpose of our study was to assess the antibiotic resistance pattern of 17 A. butzleri strains isolated in Central Italy from fresh vegetables, sushi, chicken breast, and clinical human samples to provide new and updated information about the antimicrobial resistance epidemiology of this species. Antimicrobial susceptibility testing was carried out by the European Committee on Antimicrobial Susceptibility Testing (EUCAST)'s disc diffusion method. All the strains were multidrug resistant, with 100% resistance to tetracyclines and cefotaxime (third generation cephalosporins). Some differences were noticed among the strains, according to the isolation source (clinical isolates, food of animal origin, or fresh vegetables), with a higher sensitivity to streptomycin detected only in the strains isolated from fresh vegetables. Our data, together with other epidemiological information at the national or European Union (EU) level, may contribute to developing homogeneous breakpoints. However, the high prevalence of resistance to a wide range of antimicrobial classes makes this microorganism a threat to human health and suggests that its monitoring should be considered by authorities designated for food safety
Real-world prevalence of metabolic associated fatty liver disease (MAFLD) and fibrotic-non-alcoholic steatohepatitis (NASH) in patients with obesity (PwO) in an outpatient specialist clinic in Italy
No full textsj-pdf-1-wso-10.1177_17474930241245828 – Supplemental material for Baseline clinical and neuroradiological predictors of outcome in patients with large ischemic core undergoing mechanical thrombectomy: A retrospective multicenter study
No full textSupplemental material, sj-pdf-1-wso-10.1177_17474930241245828 for Baseline clinical and neuroradiological predictors of outcome in patients with large ischemic core undergoing mechanical thrombectomy: A retrospective multicenter study by Andrea M Alexandre, Mauro Monforte, Valerio Brunetti, Luca Scarcia, Luigi Cirillo, Andrea Zini, Irene Scala, Vincenzo Nardelli, Francesco Arbia, Giuseppe Arbia, Giovanni Frisullo, Erwah Kalsoum, Arianna Camilli, Davide De Leoni, Francesca Colò, Serena Abruzzese, Mariangela Piano, Claudia Rollo, Antonio Macera, Maria Ruggiero, Elvis Lafe, Joseph D Gabrieli, Giacomo Cester, Nicola Limbucci, Francesco Arba, Simone Ferretti, Valerio Da Ros, Luigi Bellini, Giancarlo Salsano, Nicola Mavilio, Riccardo Russo, Mauro Bergui, Antonio A Caragliano, Sergio L Vinci, Daniele G Romano, Giulia Frauenfelder, Vittorio Semeraro, Maria P Ganimede, Emilio Lozupone, Andrea Romi, Anna Cavallini, Luca Milonia, Massimo Muto, Paolo Candelaresi, Paolo Calabresi, Alessandro Pedicelli and Aldobrando Broccolini in International Journal of Stroke</p
Meduza, osłona i wysłannik niebios. Tajemnice Pieśni IX „Piekła” Dantego według nadinterpretacji Giulia Leoniego w powieści „I delitti dellaMedusa”
No full textGiulio Leoni, a modern Italian writer, is the author of five crime novels inspired by the life and works of Dante Alighieri. He presents Dante as a detective who investigates mysterious crimes of the early 14th-century Florence, Rome and Venice. Although Leoni has gained an international fame, there are very few studies which examine the connections between the “Divine Comedy” and his books. My article aims to analyze the overinterpretation of Canto IX of the “Inferno” in the novel “I delitti della Medusa”.Giulio Leoni, a modern Italian writer, is the author of five crime novels inspired by the life and works of Dante Alighieri. He presents Dante as a detective who investigates mysterious crimes of the early 14th-century Florence, Rome and Venice. Although Leoni has gained an international fame, there are very few studies which examine the connections between the “Divine Comedy” and his books. My article aims to analyze the overinterpretation of Canto IX of the “Inferno” in the novel “I delitti della Medusa”
Molecular characterization and drug susceptibility of non-O1/O139 V. cholerae strains of seafood, environmental and clinical origin, Italy
No full textToxigenic and antimicrobial susceptibility patterns and genetic relatedness of 42 non-O1/O139 V. cholerae
strains, the majority of them isolated from seafood and marine water of the Adriatic sea, Italy, and 9
clinical strains, two of which with seawater of the Adriatic as the source of infection, were studied. All
strains had hlyA El Tor gene but lacked ctxA gene. Four and two isolates, respectively, also had stn/sto and
tcpA Class genes. More than 90% of strains showed susceptibility to cefotaxime, ciprofloxacin, cloramphenicol,
tetracycline, trimethoprim þ sulfamethoxazole and intermediate or full resistance to
tetracycline and erythromycin. Six strains of seafood and clinical source were multi-drug resistant. PFGE
analysis allowed to type all the strains with 50 banding patterns. Twenty-one strains, 11 and 8 from
seafood and seawater, respectively, and 2 of clinical origin, were grouped into 9 different clusters. We
report the presence of toxigenic and multidrug resistant non-O1/O139 V. cholerae strains in Adriatic,
some of which genetically related, and support that they represent a potential reservoir of toxin and
antibiotic resistance genes
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