1,720,966 research outputs found

    Problematiche sanitarie associate all’allevamento a scopo ornamentale di ciclidi: indagine anatomopatologica e molecolare di soggetti malati

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    Cichlids include several fish species that have high economic impact in the field of aquatics. These species are intensively reared and marketed, receiving great attention both at national and international level. Similarly to other rearing activities, it is important to include in the company management a health surveillance program to reduce the impact of potential diseases that could meander in the fish population. This work focused on a pathologic and molecular method-based survey in ornamental cichlids. Thirteen fish appearing debilitated or displaying aspecific signs of disease were sampled. Pathologic investigations combined with molecular analyses for identification of lymphocystis disease virus (LCDV) and granulomatogenous bacteria were carried out. Histopathologically, a granulomatous pattern, often associated to MMC hyperplasia, was the most common finding. Special stains (PAS, Perls, Ziehl-Neelsen) employed to identify the type of MMC pigments, revealed the presence of hemosiderin and ceroid-lipofuscin. Immunohistochemical detection of granulomatogenous bacteria by using anti-Mycobacterium bovis revealed them in five cases. The molecular analyses confirmed the presence of mycobacteria in two fish. The analyses for LCDV in fish affected by cutaneous nodules were negative, in accordance with the absence of hypertrophic findings affecting the dermis. The zoonotic potential of the mycobacteria detected and the employment of these animals for ornamental purposes confirm and reinforce the needing of a continuous monitoring of animals, based on the selection and study of fish in sub-optimal health status or showing aspecific signs of disease

    Swim Bladder Disorders in Koi Carp (Cyprinus carpio)

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    Swim bladder disorders and consequent buoyancy problems are encountered in ornamental fish, including koi carp. Nevertheless, beyond clinical and pharmacologicalmanagement, they are largely underdiagnosed. In this study, nine koi carp showing abdominal swelling and abnormal swimming behavior were investigated. Clinical approach, varying from case to case, included ultrasonographic and X-ray investigations, bacteriological analysis of the collected fluid, antimicrobial susceptibility pattern, and possibly histological analysis. Diagnostic imaging, corroborating gross examination, documented swim bladder deformation/dislocation and serous fluid within the swim bladder chambers of most animals. Bacteria belonging to the Aeromonas hydrophila/caviae group and Shewanella xiamenensis were identified. S. xiamenensis strains showed a sensibility to all tested molecules except for one strain, which was resistant to tetracycline and cyprofloxacin. Antibiotic treatment succeeded in the full recovery of three cases in which S. xiamemensis infection was detected. Chronic aerocystitis was histologically documented where tissue was available. The swim bladder histopathological findings highlighted a chronic process that had compromised the quality of life of the animals. A multidisciplinary clinical–pathological and microbiological approach is highly suggested to recognize swim bladder conditions as early as possible, aiming to drive medical intervention and raising the chances of fish survival

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Pathological and microbiological investigations in mortality outbreaks involving doctor fish (Garra rufa, Heckel 1843) used for cosmetic treatment

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    Introduction: Doctor fish are widely used in fish spa; their presence as living animals prohibits the use of conventional methods for water and equipment sterilization and disinfection. This has led to concerns about the potential transmission of infection to human beings and on fish welfare. The susceptibility to microbiological agents in this species is not well known, only few reports focused on episodes of Garra rufa mortality are available. The aim is to describe disease outbreaks occurred in a fish spa Materials and Methods: In the period 2016-2017 two outbreaks resulted in a chronic mortality. Collected fish were euthanized and sampled immediately for pathological, bacteriological and molecular (Infectious Pancreatic Necrosis Virus-IPNV) investigations. A classification based on timing of granulomatous lesions, in association with an acid-fast staining and immunohistochemistry against Mycobacterium bovis were performed. Results: Some animals showed rapidly growing erosive and ulcerative skin lesions. The main histopathological finding was otherwise visceral, intermediate or late stage, granulomatosis. Immunoreactivity to Mycobacterium bovis was registered. Phenotypic and genetic characterizations of isolated bacteria showed the presence of Aeromonas veronii and Vibrio cholerae in one batch (2016), Aeromonas veronii and Shewanella putrefaciens in another batch (2017). The IPNV- RNA was not found. Conclusions: The attention to granulomatogenous bacteria in aquatic species has increased because of their zoonotic potential. In the cases described, microbiological investigation associated to the pathological features led to the final diagnosis of mycobacteriosis. The presence of other bacteria has led some concerns about the use of these animals for cosmetic treatment

    Dispelling the Myths Behind First-author Citation Counts

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    We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more sophisticated methods

    Author Index

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    koamabayili/VECTRON-author-checklist: VECTRON author checklist

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    We have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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