160 research outputs found
The figure of Diomede Borghesi, between composition and staging
No full textreservedL’elaborato si prefigge di analizzare la figura di Diomede Borghesi (1540-1598), letterato senese appartenente alla seconda metà del Cinquecento, attraverso la valutazione attenta ed accurata delle sue maggiori opere in versi. Il personaggio verrà analizzato in due modalità differenti. Nella prima parte, infatti, si metterà in luce la sua figura di autore che si contraddistinse non tanto per la sua dedizione nel comporre rime, nonostante l’importante mole di versi che sono pervenuti fino ad oggi, quanto per la sua fitta rete di relazioni sociali che lo portarono a diventare allestitore di antologie e ad intrattenere rapporti con grandi personaggi del suo tempo. Tra questi ritroviamo figure di rilievo in ambito politico, culturale, ecclesiastico e militare, è necessario quindi citare Federico Gonzaga, i nobili vicentini Chieregato, Sperone Speroni, Torquato Tasso, Battista Guarini, Annibale Bonagente e Giovan Francesco Pusterla. Queste amicizie sono testimoniate dallo stesso autore attraverso la sua opera, infatti è possibile ritrovare il segno di queste relazioni all’interno delle sue numerose Lettere e nei diversi componimenti che costituiscono i libri delle sue Rime. L’analisi di queste raccolte porterà l’elaborato a dirigersi verso la conoscenza approfondita del rapporto tra Borghesi e tali personaggi del suo tempo, a cui dedica questi versi dove stende elogi o narra tratti della loro carriera politica, militare o sociale. Tra i destinatari delle rime trovano spazio anche le loro consorti o addirittura alcuni tra i loro parenti più illustri. È evidente, quindi, che nelle intenzioni dell’autore ci sia un interesse di avere un rapporto con la parte di società più eminente per ricevere onori o supporti di vario genere. Nella seconda parte della tesi, invece, la ricerca si dirigerà verso l’analisi della figura di Diomede Borghesi attraverso la sua dimensione di organizzatore delle due antologie allestite nel 1567 per l’editore Lorenzo Pasquati: Rime di diversi illustratori in lode della S. Cintia Tiene Bracciadura. Raccolte da M. Diomede Borghesi Gentil’huomo senese. Alla S. Angiola Leoni Tiene e Rime di diversi illustratori in morte del s. Lelio Chieregato. Raccolte da M. Diomede Borghesi Gentil’huomo senese. Al s. Valerio Chieregato Cavalliero. La schedatura e l’analisi delle due antologie permetteranno di mettere in evidenza la dimensione della poesia e dell’encomio nella contemporaneità trattata. Inoltre, grazie all’attenzione rivolta all’organizzazione delle due raccolte, sarà possibile ricostruire le dinamiche sociali e culturali che governavano l’epoca. Ad aiutare nella ricerca sarà l’attenta valutazione della lingua utilizzata nelle rime e gli elementi topici che sorgono dal confronto di questi componimenti scritti da diversi autori, che sono tuttavia immersi nella stessa realtà sociale e temporale.This paper aims to analyze Diomede Borghesi (1540-1598), a Sienese man of letters from the second half of the sixteenth century, through the careful evaluation of his major works in verse. The character will be analyzed in two different ways. In the first part we will highlight his figure as the one of an author who distinguished himself not so much for his dedication in composing rhymes, despite the important amount of verses that have come down to us, but as for his dense network of social relations that led him to become the organizer of anthologies and to entertain relationships with major figures of his time. Among these we find important figures in the political, cultural, ecclesiastical and military spheres, it is therefore necessary to mention Federico Gonzaga, the Vicenza nobles Chieregato, Sperone Speroni, Torquato Tasso, Battista Guarini, Annibale Bonagente and Giovan Francesco Pusterla. These friendships are witnessed by the author himself through his work, in fact it is possible to find the sign of these relationships within his numerous Letters and in the different compositions that constitute the books of his "Rime". The analysis of these collections will lead the student to an in-depth knowledge of the relationship between Borghesi and these personalities of his time, to whom he dedicates these verses where he praises or narrates traits of their political, military or social career. Among the recipients of the rhymes are also their spouses or even some of their most illustrious relatives. It is evident, therefore, that in the author's intentions there is an interest in having a relationship with the most eminent part of society in order to receive honors or support of various kind. In the second part of the thesis, instead, the research will be directed towards the analysis of the figure of Diomede Borghesi through his dimension of organizer of the two anthologies prepared in 1567 for the publisher Lorenzo Pasquati: "Rime di diversi illustratori in lode della S. Cintia Tiene Bracciadura. Raccolte da M. Diomede Borghesi Gentil’huomo senese. Alla S. Angiola Leoni Tiene" and "Rime di diversi illustratori in morte del s. Lelio Chieregato. Raccolte da M. Diomede Borghesi Gentil’huomo senese. Al s. Valerio Chieregato Cavalliero". The cataloguing and analysis of the two anthologies will make it possible to highlight the dimension of poetry and encomium in the contemporaneity treated. Moreover, thanks to the attention paid to the organization of the two collections, it will be possible to reconstruct the social and cultural dynamics that governed the period. To help in the research there will be the careful examination of the language used in the rhymes and the topical elements that arise from the comparison of these compositions written by different authors, who are however immersed in the same social and temporal reality
Table_S2 - The Role of Hypoxia on the Neuronal Differentiation of Gingival Mesenchymal Stem Cells: A Transcriptional Study
No full textTable_S2 for The Role of Hypoxia on the Neuronal Differentiation of Gingival Mesenchymal Stem Cells: A Transcriptional Study by Agnese Gugliandolo, Francesca Diomede, Domenico Scionti, Placido Bramanti, Oriana Trubiani and Emanuela Mazzon in Cell Transplantation</p
Table_S1_(1) - The Role of Hypoxia on the Neuronal Differentiation of Gingival Mesenchymal Stem Cells: A Transcriptional Study
No full textTable_S1_(1) for The Role of Hypoxia on the Neuronal Differentiation of Gingival Mesenchymal Stem Cells: A Transcriptional Study by Agnese Gugliandolo, Francesca Diomede, Domenico Scionti, Placido Bramanti, Oriana Trubiani and Emanuela Mazzon in Cell Transplantation</p
HEMA Effects on Autophagy Mechanism in Human Dental Pulp Stem Cells
No full textAutophagy is a complex mechanism that permits the degradation of cellular components in order to enhance cell homeostasis, recycling the damaged, dysfunctional, or unnecessary components. In restorative dentistry practice, free resin monomers of 2-hydroxyethyl methacrylate (HEMA) can be released. The aim of this study was to investigate the effect of HEMA on proliferation and autophagy in human dental pulp stem cells (hDPSCs). Human DPSCs were treated with different concentrations of HEMA (3 and 5 mmol L−1). To evaluate the proliferation rate, MTT and trypan blue assays were used. Autophagic markers such as microtubule-associated protein 1 light chain 3 (LC3-I/II) and ubiquitin-binding protein (p62) were analyzed through immunofluorescence observations. Beclin1, LC3-I/II, and p62 were evaluated by means of Western blotting detection. Considering that activity of extracellular signal–regulated kinase (ERK) and its phosphorylated form (pERK) mediates several cellular processes, such as apoptosis, autophagy, and senescence, the involvement of ERK/pERK signaling was also evaluated. Obtained results showed a decreased cell proliferation associated with morphological changes in HEMA-treated cells. The Western blot results showed that the expression levels of Beclin1, LC3-I/II, and ERK were significantly elevated in HEMA-treated cells and in cells co-treated with rapamycin, an autophagic promoter. The expression levels of p62 were significantly reduced compared to the untreated samples. Protein levels to the autophagic process, observed at confocal microscopy confirmed the data obtained from the Western blot. The up-regulation of ERK and pERK levels, associated with nuclear translocation, revealed that ERK pathway signaling could act as a promoter of autophagy in dental pulp stem cells treated with HEMA
MicroRNA 210 Mediates VEGF Upregulation in Human Periodontal Ligament Stem Cells Cultured on 3DHydroxyapatite Ceramic Scaffold
No full textThe aim of the present research was the evaluation of the behavior of human periodontal ligament stem cells (hPDLSCs), cultured in presence of Endobon® Xenograft Granules (G), a fully deproteinated hydroxyapatite ceramic scaffold derived from cancellous bovine bone. hPDLSCs were seeded with and without G for 24 h to 1 week. The cell growth, morphological features, adhesiveness, differentiation ability, modulation of miR-210 and Vascular Endothelial Growth Factor (VEGF) secretion were analyzed by means of MTT assay, Scanning Electron Microscopy (SEM), Confocal Laser Scanning Microscopy (CLSM), Alizarin Red S assay, RT-PCR and ELISA test, respectively. hPDLSCs grown on the biomaterial showed the ability to form focal adhesion on the substrate, as demonstrated by vinculin expression. These data were supported by SEM analysis showing that an adhesiveness process associated to cell growth occurs between cells and biomaterials. The osteogenic differentiation, evaluated by morphological, biochemical, and RT-PCR analysis, was pronounced in the hPDLSCs grown in the three-dimensional inorganic bovine bone substitute in the presence of osteoinductive conditions. In addition, an upregulation of miR-210 and VEGF was evident in cells cultured in presence of the biomaterial. Our results inspire us to consider granules not only an adequate biocompatible three-dimensional biomaterial, but also an effective inductor of miR-210 and VEGF; in fact, the involvement of miR-210 in VEGF secretion could offer a novel regulatory system in the early steps of the bone-regeneration process
Anti‐inflammatory effects of hypoxia‐preconditioned human periodontal ligament cell secretome in an experimental model of multiple sclerosis: a key role of IL‐37
No full textRecent research has widely investigated the anti-inflammatory effects of mesenchymal stem cells and their secretory products, termed the secretome, in the treatment of multiple sclerosis (MS). The present study examined the capacity of the conditioned medium (CM) from human periodontal ligament stem cells (hPLSCs) under hypoxia (H-hPDLSCs-CM) to suppress experimental autoimmune encephalomyelitis (EAE), a murine model of MS. To induce EAE, female C57BL/6 mice were immunized with myelin oligodendroglial glycoprotein peptide35-55 At the onset of symptoms, H-hPDLSCs-CM was infused via the tail vein of mice. Our results demonstrate the efficacy of H-hPDLSCs-CM treatment in diminishing clinical and histologic disease score. A key finding from this study is the marked expression of anti-inflammatory cytokine IL-37, paralleled by the suppression of proinflammatory cytokines in mice with EAE that were treated with H-hPDLSCs-CM. In addition, a consequent modulation of oxidative stress, autophagic, and apoptotic markers was observed in mice with EAE after hPDLSCs-CM administration. In addition, to provide additional evidence of the molecular mechanisms that underlie H-hPDLSCs-CM, we investigated its therapeutic action in scratch injury-exposed NSC-34 neurons, an in vitro model of injury. This model reproduces severe inflammation and oxidative stress conditions as observed after EAE damage. In vitro results corroborate the ability of hPDLSCs-CM to modulate inflammatory, oxidative stress, and apoptotic pathways. Taken together, our findings suggest H-hPDLSCs-CM as a new pharmacologic opportunity for the management of MS.-Giacoppo, S., Thangavelu, S. R., Diomede, F., Bramanti, P., Conti, P., Trubiani, O., Mazzon, E. Anti-inflammatory effects of hypoxia-preconditioned human periodontal ligament cell secretome in an experimental model of multiple sclerosis: a key role of IL-37
Evaluation of pro-inflammatory cytokines, and cell growth inhibition in primary human cells culture from oral tissue treated with uretandimethacrylate (UDMA)
No full textImmunomodulating Profile of Dental Mesenchymal Stromal Cells: A Comprehensive Overview
No full text: Dental mesenchymal stromal cells (MSCs) are multipotent cells present in dental tissues, characterized by plastic adherence in culture and specific surface markers (CD105, CD73, CD90, STRO-1, CD106, and CD146), common to all other MSC subtypes. Dental pulp, periodontal ligament, apical papilla, human exfoliated deciduous teeth, alveolar bone, dental follicle, tooth germ, and gingiva are all different sources for isolation and expansion of MSCs. Dental MSCs have regenerative and immunomodulatory properties; they are scarcely immunogenic but actively modulate T cell reactivity. in vitro studies and animal models of autoimmune diseases have provided evidence for the suppressive effects of dental MSCs on peripheral blood mononuclear cell proliferation, clearance of apoptotic cells, and promotion of a shift in the Treg/Th17 cell ratio. Appropriately stimulated MSCs produce anti-inflammatory mediators, such as transforming growth factor-β (TGF-β), prostaglandin E2, and interleukin (IL)-10. A particular mechanism through which MSCs exert their immunomodulatory action is via the production of extracellular vesicles containing such anti-inflammatory mediators. Recent studies demonstrated MSC-mediated inhibitory effects both on monocytes and activated macrophages, promoting their polarization to an anti-inflammatory M2-phenotype. A growing number of trials focusing on MSCs to treat autoimmune and inflammatory conditions are ongoing, but very few use dental tissue as a cellular source. Recent results suggest that dental MSCs are a promising therapeutic tool for immune-mediated disorders. However, the exact mechanisms responsible for dental MSC-mediated immunosuppression remain to be clarified, and impairment of dental MSCs immunosuppressive function in inflammatory conditions and aging must be assessed before considering autologous MSCs or their secreted vesicles for therapeutic purposes
Come le foglie
No full textFrancesca Favaro Vuoi ribellarti contro le foglie che il vento disperde? Trattienile se puoi. Hanno tanta grazia, e tanta eleganza, e non sai dove vanno a finire. (Giuseppe Giacosa, Come le foglie, Atto III, scena X) Due guerrieri si fronteggiano, sotto le mura di Ilio. Il loro sarà uno degli aristocratici duelli – singole, fulgide prove di coraggio – da cui sono intervallate più vaste battaglie. Si accingono allo scontro e l’uno, il greco Diomede, domanda all’altro, il Troiano, quale sia la ..
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