1,720,982 research outputs found
Investigation of long non-coding RNA expressions in tyrosine kinase inhibitors sensitive and resistant chronic myeloid leukemia patients at molecular level
No full textUzun kodlamayan RNA' lar (lncRNA), ortalama 200-100 kbp uzunluğunda, hücrelerde gen ifadesinin epigenetik düzeyde kontrolünden sorumlu olan RNA molekülleridir. LncRNA' ların ekspresyon düzensizliği çeşitli insan kanser tipleri ile ilişkilidir. Ancak, Kronik myeloid lösemi' de (KML) ve özellikle tirozin kinaz inhibitörlerine karşı oluşan dirençte lncRNA' ların fonksiyonları tam olarak bilinmemektedir. Uzun kodlamayan RNA NEAT1 çeşitli kanser türlerinde önemli role sahip olup, iki kısa izoforma sahiptir: kısa form NEAT1_1 ve uzun form NEAT1_2. NEAT1 aynı zamanda nukleusta sub bodies olan 'paraspeckles' ve microspeckles oluşumundan da sorumludur. Bu çalışmanın amacı KML'de ve tirozin kinaz inhibitörlerine karşı gelişen direnç mekanizmasında etki gösteren lncRNA ekspresyon profillerinin belirlenmesi ve belirlenen lncRNA'ların TKİ direnç mekanizmasında ve KML progresyonundaki rollerinin saptanmasıdır. Dolayısıyla KML'de gen ifadesini direkt veya dolaylı şekilde etkileyen lncRNA' ların profillenmesi, sağlıklı olgulardan ve tirozin kinaz inhibitörlerine duyarlı/dirençli kronik myeloid lösemi hastalarından elde edilen kan örneklerinden RNAeasy Mini kit (Qiagen) ile total RNA izolasyonu, 'Thermocycler' cihazında qRT-PCR (kantitatif revers transkriptaz-gerçek zamanlı polimeraz zincir reaksiyonu) yöntemi ile cDNA (komplemanter DNA) sentezi ve 'ABI 7500 Fast' cihazında qRT-PCR (gerçek zamanlı polimeraz zincir reaksiyonu) array ile yapılmıştır. Farklı ekspresyon seviyeleri gösteren lncRNA'lar potansiyel terapötik hedefler olabileceğinden, lncRNA' ları hedefleyen tedavi stratejisinin tirozin kinaz inhibitörlerine karşı direnç gösteren KML hastalarının tedavisi için de, umut verici nitelikte olacağını düşünmekteyiz.Long non-coding RNAs (lncRNAs) are non-coding RNAs that are > 200nt, and many have been found to be involved in many epigenetic mechanisms such as chromatin modification, genomic imprinting, and regulation of gene expression. Deregulated expression of Nuclear paraspeckle assembly transcript 1 (NEAT1) has been found to be related with the progression of several cancers. The expression of NEAT1 normally results in the production of a class of nuclear RNA-protein complex named 'paraspeckles' in mammalian nucleus. The function of NEAT1 and 'paraspeckles' in the BCR-ABL associated pathways (AKT, ERK, STAT pathways) in KML still remains largely unknown, but recent studies showed NEAT1 overexpression in leukemia cell line reduced MDR (multi drug resistance) induced pathways in KML. In this study we aimed to investigate the role of lncRNA NEAT1 in BCR-ABL mediated tumorigenesis in CML. We hypothesize that NEAT1 might target imatinib resistance-associated genes and downstream of the BCR-ABL fusion protein genes (PI3K / Akt, mTOR, JAK-STAT) in CML. For this reason, our study may contribute to an elucidation of imatinib response and imatinib resistance mechanisms in association with NEAT1 and 'paraspeckles' in CML.Furthermore, since BCR/ABL was found to translocate from the cytoplasm to the nucleus following genotoxic stress, and then associates with ATR-dependent DNA damage response pathways, which is also found to be regulated by 'paraspeckles' and NEAT1 expression, it becomes intriguing to test if NEAT1 might be a novel target for the treatment of chronic myeloid leukemia
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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