35,070 research outputs found
Isolation of novel antibiotic producing microorganisms from invertebrates
No full textBecause of the increased incidence of antibiotic resistance there is an increasing need to isolate new microorganisms producing new antibiotics. Because conventional sources such as soil have been exhausted, attention has been turned to unexplored sources. The aims of this research were to investigate terrestrial and aquatic invertebrates as a source of antibiotic producing microorganisms, to isolate the organisms and screen them for activity against Gram-positive and Gram-negative bacteria. Those with activity would be further characterised. Forty-seven antibiotic producers were isolated from 35 samples of different terrestrial and aquatic invertebrates using media designed to isolate streptomycetes. Identification by PCR and sequencing of 16S and 18S rRNA showed that the majority were in fact fungi, mainly Penicillium spp. and only 5 were Streptomyces spp. Three strains isolated from Helix aspersa (ES1), Lumbriculus variegate (La1a) and Baetis larvae (M1 11) that had strong activity against Gram-positive and Gram-negative bacteria including Methicillin-resistant Staphylococcus aureus (MRSA) were chosen for further study. PCR and sequencing of rRNA and Internal Transcribed Spacer (ITS) regions confirmed that the strains were closely related and were probably strains of P. chrysogenum. The organisms were grown in liquid media and on agar and the active components were extracted with ethyl acetate and fractionated by HPLC. As facilities for large scale liquid cultures were not available large scale preparation was performed from agar medium and the active fractions. HPLC showed that the extracts were complex and contained more than one active component. The purified fractions were chemically characterised by NMR, FTIR and MS. The results showed that the active components were novel flavones and isoflavones. Such compounds have extracted from plants and only previously been shown to be produced by endophytic fungi. The minimal inhibitory concentrations (MIC) against test bacteria and inhibitory concentration IC50 (IC50 is the concentration of a compound at which 50 % of human cancer cell tissue cultures are killed) showed potential for use in chemotherapy as all purified fractions except Es1 F3/3 and Es1 F6 were found to have IC50 values>100 μg/ml, High IC50 and low MIC (0.39 to 50 μg/ml) may indicate fractions suitable for testing for treatment of human infections. The results showed that the invertebrates were indeed a source of antibiotic producing organisms. Whether the organisms were acquired by the invertebrates from the environment or whether they were in a symbiotic association needs further study
Ha-Dibbur Ha-Ivri
No full textHebrew Speech, a manual for beginners for the study of Hebrew according to the Natural Method). Author: M. Krinsky. Illustrator: H. Goldberg. Publisher: Ha-Or Publishing. . 146 pp. 254 b/w ills. (+1 color chart) + cover b/w ill. primer.Digital imagedigitize
Author Ha Jin at Kalamazoo College
No full textPhotographer unknown.Author Ha Jin's book "Waiting" was selected for the 2002 Summer Common Reading program at Kalamazoo College. The author visited campus that year as part of the program
Developing A Marketing Recruitment Strategy For International Business
No full textOver the last 20 years, recruiting strategies have changed considerably due to the expansion of technology and the Internet. Human resources are also contemplated as the core competitive factor of businesses. This research is conducted to systematize the theoretical basis related to recruitment marketing. Moreover, at various phases, it employs both qualitative and quantitative research approaches.
In terms of the empirical component, the owner of the corporate case provided qualitative data. In contrast, quantitative data from possible candidates were reviewed. The theoretical portion gives the audiences a fundamental understanding of recruiting and the use of a recruitment marketing strategy. The author researched some surveys and case studies to gather data from both employees and employers.
On the whole, the research findings disclosed the importance of recruitment marketing to international enterprises. Businesses should focus on recruitment marketing to improve recruitment efficiency and reach a bigger pool of potential talents. In the end, the author designated some recommendations for businesses to improve recruitment marketing effectiveness
Foster care and social work services from the perspective of the foster child
No full textIncludes bibliographical references
Estimating population diversity with CatchAll
No full textMotivation: The massive data produced by next-generation sequencing require advanced statistical tools. We address estimating the total diversity or species richness in a population. To date, only relatively simple methods have been implemented in available software. There is a need for software employing modern, computationally intensive statistical analyses including error, goodness-of-fit and robustness assessments. Results: We present CatchAll, a fast, easy-to-use, platform-independent program that computes maximum likelihood estimates for finite-mixture models, weighted linear regression-based analyses and coverage-based non-parametric methods, along with outlier diagnostics. Given sample ‘frequency count’ data, CatchAll computes 12 different diversity estimates and applies a model-selection algorithm. CatchAll also derives discounted diversity estimates to adjust for possibly uncertain low-frequency counts. It is accompanied by an Excel-based graphics program. Availability: Free executable downloads for Linux, Windows and Mac OS, with manual and source code, at www.northeastern.edu/catchall. <br/
Interphase chromosome positioning in in vitro porcine cells and ex vivo porcine tissues
No full textCopyright @ 2012 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and 85 reproduction in any medium, provided the original author and source are credited. The article was made available through the Brunel University Open Access Publishing Fund.BACKGROUND: In interphase nuclei of a wide range of species chromosomes are organised into their own specific locations termed territories. These chromosome territories are non-randomly positioned in nuclei which is believed to be related to a spatial aspect of regulatory control over gene expression. In this study we have adopted the pig as a model in which to study interphase chromosome positioning and follows on from other studies from our group of using pig cells and tissues to study interphase genome re-positioning during differentiation. The pig is an important model organism both economically and as a closely related species to study human disease models. This is why great efforts have been made to accomplish the full genome sequence in the last decade. RESULTS: This study has positioned most of the porcine chromosomes in in vitro cultured adult and embryonic fibroblasts, early passage stromal derived mesenchymal stem cells and lymphocytes. The study is further expanded to position four chromosomes in ex vivo tissue derived from pig kidney, lung and brain. CONCLUSIONS: It was concluded that porcine chromosomes are also non-randomly positioned within interphase nuclei with few major differences in chromosome position in interphase nuclei between different cell and tissue types. There were also no differences between preferred nuclear location of chromosomes in in vitro cultured cells as compared to cells in tissue sections. Using a number of analyses to ascertain by what criteria porcine chromosomes were positioned in interphase nuclei; we found a correlation with DNA content.This study is partly supported by Sygen International PLC
Abitare - Being Norman Foster
No full textLa Monografia Abitare - Being Norman Foster è il risultato di tre mesi vissuti a stretto contatto con Norman Foster nei suoi viaggi internazionali, sui cantieri a New York e Masdar, durante i sopralluoghi a Pechino e Madrid, alle inaugurazioni di edifici e di mostre, negli studi più importanti che ha aperto nel mondo.
Abitare - Being Norman Foster - è il secondo monografico di Abitare - Being...che ha da subito ricevuto ampio riscontro sia nell’editoria tradizionale sia nell’applicazione per iPad, che ha ricevuto il Premio Moebius Multimedia 2010 (primo premio nella categoria ebook)
All of Life as Worship, with Theological Librarian, PhD Candidate, and Author Sam Ha
No full textSam Ha, PhD Candidate and Meeter Center for Calvin Studies Curator, recently released his book, A Day of Worship: Adoring God in Every Moment. The book is a pastorally-rich, theologically in-depth exploration of how each moment of life-mundane and special-can be a form of worship before God. Within its first week, it became the 2nd most sold book in Christianity & theology books in Sam’s home country of Korea
Semiometrics: producing a compositional view of influence
No full textHigh-impact academic papers are not necessarily the most cited. For example, Einstein's 'Special Relativity' paper from 1905 received (and continues to receive) fewer citations from other papers than his 'Brownian Motion" paper of the same year, despite the former radically changing the course of an entire scientific discipline to a much greater extent. Similarly, 'impact' metrics using citation count alone are, it is argued, not adequate for determining the scientific influence of papers, authors or small groups of authors. Although valid, they remain controversial when used to determine influence of larger groups or journals. While the term 'impact' has become closely linked to a journal's citation-based Journal Impact Factor score, this thesis uses the term 'influence' to describe the wider effectiveness of research, combining citation and metadata analysis to allow richer calculations to be performed over large-scale document networks. As a result, more qualitative influence ratings can be determined and a broader outlook on scientific disciplines can be produced. These ratings are best applied using an ontology-based data source, allowing more efficient inference than under a traditional RDBMS system, and allowing easier integration between heterogeneous data sources. These metrics, termed 'Semantic Bibliometrics' or 'Semiometrics', can be applied at a variety of levels of granularity, allowing a compositional framework for impact and influence analysis. This thesis describes the process of data preparation, systems architecture, metric value and data integration for such a system, introducing novel approaches at all four stages, thereby creating a working semiometrics system for determining influence at different semantic levels of granularity
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