1,721,053 research outputs found
Ultrastructural Examination of a Case of Pagetoid Bowen Disease Exhibiting Immunohistochemical Features in Common With Extramammary Paget Disease.
No full text
Actin isoform pattern expression: a tool for the diagnosis and biological characterization of human rhabdomyosarcoma
No full textSquamous cell carcinoma with prominent myxoid stroma.
No full textThree cases of a squamous cell carcinoma with a prominent myxoid stroma are reported. One case in a 70-year-old woman had presented as a lump in the breast, the other two presented as polypoid lesions of the larynx and cervix uteri in a 65-year-old man and 61-year-old woman, respectively. The carcinomatous component was immersed in abundant extracellular mucosubstances. In addition to occasional squamous pearls, it displayed immunocytochemical evidence of high-weight keratin present in the neoplastic cells and, in one case, desmosomal attachment under electron microscopy. The extracellular mucosubstances proved to be similar to those seen in connective tissue. The differential diagnosis with histologically similar lesions has been taken into consideration, and it has been suggested that this newly described entity showing abundant myxoid stroma has to be distinguished from numerous benign and malignant myxoid soft tissue tumors
Prognostic impact of DNA methylation analysis in adjacent area of surgically resected oral squamous cell carcinoma during follow up
No full textBackground & Objectives: Oral Squamous Cell Carcinoma (OSCC) showed a significant risk to develop local recurrences or second primary tumours during follow-up. Recently, we developed a non-invasive assay based on oral brushing and DNA methylation analysis to early detect OSCC. In the present study using this assay, we investigated the adjacent area of surgical resection in a series of OSCC during follow-up for prog- nostic purposes.
Methods: 42 consecutive OSCC patients were sampled during routine follow-up after 6 months from surgical treatment, brushing the regenera- tive mucosa covering the region that underwent the surgical OSCC exci- sion. DNA methylation level of ZAP70, GP1BB, KIF1A, ITGA4, LINC00599, MIR193, MIR296, TERT, LRRTM1, NTM, EPHX3, FLI1 and PARP15 was evaluated by quantitative Bisulfite-NGS. After calcu- lating a score by Linear-Discriminant-Analysis, the samples were dichot- omized using a predefined threshold previously developed for early di- agnosis. One-Way-ANOVA and Kaplan-Meier curves served to evaluate any significant difference between patients who experienced a second neoplastic manifestation and the group who did not.
Results: 6/42 (14,3%) patients developed a second neoplastic manifesta- tion during follow-up period (mean follow-up: 14.3 months), of which 5 showed a positive methylation score. Additional 11 patients exceeded the threshold but up to date they have not experienced any second manifes- tation. Among the remaining 26 negatives, only one developed a recur- rence. A positive score correlated with a worse locoregional control of disease (p<0.05).
Conclusion: The DNA methylation analysis of 13 genes can be a useful non-invasive method to identify surgically treated OSCC patients at risk of developing a second neoplasia
The Milan System for Reporting Saliavry Gland Cytopathology
No full textSummary of inadequate pictures in saliavry gland cytopatholog
Utility of eco-colour doppler sonography in the selection of thyroid nodules for fine-needle aspiration cytology
No full textBreast tumor resembling the tall cell variant of papillary thyroid carcinoma: Report of 4 cases with evidence of malignant potential
No full textA new type of breast carcinoma resembling the tall cell variant of papillary thyroid carcinoma has recently been described. To date, rare cases are on record. Here, 4 new cases of the tall cell variant of papillary thyroid carcinoma of the breast are described in women aged from 45 to 80 years old. All patients presented with palpable breast nodules and were treated with quadrantectomy One patient presented with a long clinical history and a metastatic intramammary lymph node. The patient is alive and well 3 months after surgery. The remaining 3 patients are disease free at mean 7.5 months (range, 5 to 10 months) after surgery These data suggest that papillary thyroid-like carcinomas of the breast show malignant potential
Congenital cytomegalovirus infection in twin pregnancies: viral load in the amniotic fluid and pregnancy outcome
No full textHuman cytomegalovirus (CMV) is the most common cause of viral intrauterine infection and fetal damage largely attributable to maternal primary infection. Most cases of congenital CMV infection in twins reported in the literature involved only 1 twin. We assessed the validity of polymerase chain reaction (PCR) and quantitative PCR on amniotic fluid (AF), at 21 to 22 weeks' gestation and at least 6 to 8 weeks after seroconversion, to predict the outcome of newborns in twin pregnancies. Two pregnant women with twin pregnancies and 1 woman with a triple pregnancy with primary CMV infection defined by the presence of immunoglobulin (Ig) M and low IgG avidity and/or by the presence of clinical symptoms and abnormal liver enzyme values were evaluated. CMV infection was found in 6 fetuses/newborns, 3 of whom were symptomatic. In the first twin pregnancy with diamniotic-dichorionic separate placentas, CMV symptomatic infection of the female twin was demonstrated by positive virus isolation and high viral load in AF. The male fetus was not infected as demonstrated by negative CMV culture and DNA detection in AF. In the triple pregnancy, the woman had a placenta with 2 monozygotic twins (females) and a separate placenta with a heterozygotic twin (male). The quantitative PCR results were 103 genome equivalents (GE)/mL of females AF and 1.9x10(5) GE/mL of male AF. Both female twins were asymptomatic at birth, whereas the male presented petechiae, thrombocytopenia, and cerebral ventriculomegaly. In the last twin pregnancy with fused dichorionic placentas, congenital CMV infection of both twins was diagnosed at birth in contrast with prenatal diagnosis. At time of amniocentesis, the left side twin was not infected as shown by negative results of CMV culture and DNA detection in the AF. CMV infection of the right side twin was demonstrated by positive CMV DNA detection with a CMV DNA load of 4.9x10(4) GE/mL and positive virus isolation in the AF. The morphologic and histologic examinations of the placentas strongly supported a prenatal horizontal acquisition of CMV infection. These twin pregnancies showed a marked difference in the quantity of virus load documented by the prenatal diagnosis suggesting that twin fetuses may react differently to primary maternal infection despite being exposed to the same maternal influences. A high viral load is correlated with congenital CMV infections symptomatic at birth. In such cases, with fetal infection of only 1 twin (at amniocentesis) and fusion of placentas, fetal outcome of both twins needs to be evaluated for the possibility of viral transfer from one fetus to the other
- …
