102,130 research outputs found
Dal carteggio all'erabrio di Carlo Allioni (1728-1804): le relazioni locali e internazionali del Botanico Piemontese
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Similar sequence-free amplification of human glyceraldehyde-3- phosphate dehydrogenase for real time RT-PCR applications
No full textOne of the major applications of real time polymerase chain reaction (PCR) is
relative quantification, where the expression of a target gene is determined as a
ratio to a stably expressed reference gene, the so-called housekeeping gene.
Glyceraldehyde-3-phosphate dehydrogenase (GAPD) is a glycolytic enzyme, which is
active in all mammalian tissues and is frequently used as housekeeping gene in
expression studies. The functional locus maps to human chromosome 12p13, but
several GAPD-related sequences, including processed pseudogenes, GenBank
homologous sequences and computationally predicted sequences are present along
the human genome. Due to the high level of GAPD-related sequences it is very
important to avoid genomic DNA amplification when GAPD is used as endogenous
control in mRNA quantification. We have outlined a GAPD couple of primers that
avoid any genomic DNA amplification for real time reverse transcription PCR
applications by SYBR-Green Dye. These new designed primers are an useful and chip
alternative to probe technologies, and can carry out specific and reproducible
data in mRNA expression studies
Il recupero di aree marginali con il pascolamento: approccio metodologico alla carta della vegetazione in scala 1:5.000 del Monte Pavaglione (Appennino ligure)
No full textNutritional Value of farmed fish and human healt. Food and Health, Role of Animal Products
No full textEffects of supplementing with red wine solids on the oxidative status in pigs fed diets with different fatty acid profiles
No full textStructural characterization of the third scavenger receptor cysteine-rich domain of murine Neurotrypsin
No full textNeurotrypsin (NT) is a multi-domain serine protease of the nervous system with only one known substrate: the large proteoglycan Agrin. NT has seen to be involved in the maintenance/turnover of neuromuscular junctions and in processes of synaptic plasticity in the central nervous system. Roles which have been tied to its enzymatic activity, localized in the C-terminal serine-protease (SP) domain. However the purpose of NT's remaining 3-4 scavenger receptor cysteine-rich (SRCR) domains is still unclear. We have determined the crystal structure of the third SRCR domain of murine NT (mmNT-SRCR3), immediately preceding the SP domain and performed a comparative structural analysis using homologous SRCR structures. Our data and the elevated degree of structural conservation with homologous domains highlight possible functional roles for NT SRCRs. Computational and experimental analyses suggest the identification of a putative binding region for Ca2+ ions, known to regulate NT enzymatic activity. Furthermore, sequence and structure comparisons allow to single out regions of interest that, in future studies, might be implicated in Agrin recognition/binding or in interactions with as of yet undiscovered NT partners. This article is protected by copyright. All rights reserved
The application of Lake Shorezone Functionality Index (SFI): a valid tool to assess the reproductive sites status of bleak (Alburnus arborella)
No full textCalcitonin gene-related peptide (CGRP), acting via CGRP type 1 receptors, inhibits potassium-stimulated aldosterone secretion and enhances basal catecholamine secretion from rat adrenal gland
No full textCalcitonin gene-related peptide (CGRP) is a potent hypotensive peptide, that acts via two main subtypes of receptors, named CGRP1 and CGRP2. CGRP belongs to a regulatory-peptide family, that includes adrenomedullin (ADM) whose aldosterone antisecretagogue and catecholamine secretagogue actions are well demonstrated. Quantitative autoradiography showed the presence of [125I]CGRP binding sites in both rat adrenal zona glomerulosa (ZG) and medulla. Binding was displaced by the CGRP1-receptor antagonist CGRP(8-37), but not by the CGRP2-receptor agonist [cys(Et)2,7]-alphaCGRP (CGRP2-A). CGRP concentration-dependently inhibited 10 mM-stimulated (but not basal) aldosterone secretion from dispersed rat ZG cells, and enhanced basal catecholamine secretion from rat adrenomedullary fragments. The responses to the maximal effective concentration of CGRP (10-8 M) were blocked by 10-7 M CGRP(8-37). CGRP2-A (10-7 M) neither altered aldosterone response to 10 mM K+ nor enhanced basal catecholamine secretion. The conclusion is drawn that CGRP, like ADM, inhibits agonist-stimulated aldosterone secretion and stimulates basal catecholamine release in the rat, exclusively acting via CGRP1 receptors
Bibliographie Hilarion G. Petzold 1958 – 2009 mit Anhang als Einführung
No full textDieses Archiv enthält die Gesamtbibliographie der Werke des Autors nebst einiger Texte „Über H. G. Petzold“ im Schlussteil der Bibliographie sowie einen Anhang mit einer Einführung in die Architektur des Werkes in seinem wissenslogischen Aufbau als Ausarbeitung seines „Tree of Science Modells“ (2007).This archive contains the complete bibliography of the author and some texts about H. G. Petzold, moreover an epilogue with an introduction to the architecture of the works in its epistemological structure and composition and as an elaborations of Petzold’s „Tree of Science Modell (2007).https://www.fpi-publikation.de/polyloge/01-2009-petzold-h-g-gesamtbibliographie-h-g-petzold-1958-2009-updating-november2009/peerReviewedpublishedVersio
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