1,720,996 research outputs found
Cutaneous arthropod reaction in the dog: six cases
No full textSponsors; Organizing Committees; Preface; List of contents; PART 1: ENVIRONMENT & THE SKIN: The light and the skin; Aquatic problems and piscine solutions; The intimate envelope - the microclimate of the canine skin and coat; The application of infrared tympanic membrane thermometry in comparing the external ear canal temperature between erect and pendulous ears in dogs; Vitiligo following melanoma in Vietnamese pot-bellied pigs - gross, ultrastructural and immunological studies; PART 2: IMMUNOLOGICAL REAGENTS IN VETERINARY DERMATOLOGY: The use of immunological reagents in defining the pathogenesis of skin diseases; The effect of cyclosporin A on acquired immunity to orf virus reinfection - inhibition of T cell recruitment and activation; Immunohistochemical characterization of canine acute graft-versus-host disease and erythema multiforme; Feline Langerhans cells and their possible role in FIV infection; Immunophenotypic and ultrastructural characterization of feline mucocutaneous Langerhans cells; Gamma-delta T cells in normal and diseased canine skin; PART 3: ALLERGIC SKIN DISEASES: Advances in allergic skin diseases; Aqueous hyposensitization in the treatment of atopic dermatitis - a retrospective and prospective study of 100 cases; The pathogenesis and immunopharmacology of equine insect hypersensitivity; Responses in horses to intradermal challenge of insects and environmental allergens with specific immunotherapy; Salivary allergens of Ctenocephalides felis - collection, purification and evaluation by intradermal skin testing in dogs; Ultrastructural changes in feline skin mast cells during antigen-induced degranulation in vivo; Detection of mediator release from individual mast cells using a Novel ELISPOT Assay; PART 4: METABOLIC AND ENDOCRINE SKIN DISEASES: Effect of Trimethoprim-sulfamethoxazole on endogenous thyroid stimulating hormone concentrations in dogs; Diet-induced alterations in lipid metabolism and associated cutaneous xanthoma formation in five cats; The postprandial plasma concent
Indirect immunofluorescence (IFI) on kinetoplasts of trypanosoma theileri for the diagnosis of sistemic lupus erithemathosus (LES) in the dog.
No full textImmunohistochemical and molecular investigation on the presence of Leishmania spp. in the skin of cats with head and neck ulcers
No full textBackground: Head and neck ulcers in cats are frequently due to allergic disorders but are also reported in feline leishmaniosis, a disease in which drugs used to treat allergy are contraindicated. Prevalence of Leishmania infection in cats from endemic regions is not negligible and parasitic DNA, but not amastigotes, was recently demonstrated in diseased and normal feline skin.
Objective: The aim of this study was to investigate, through immunohistochemical and molecular techniques, the presence of Leishmania in skin of cats with head and neck ulcers.
Methods: A retrospective histopathological, immunohistochemical and molecular study was conducted on paraffinembedded skin samples from 29 cats, most of them living in areas of Italy at potential risk for leishmaniosis, presenting
head and neck ulcerative dermatitis. Specimens were routinely stained with haematoxylin-eosin, immunostained using a polyclonal anti-Leishmania antibody and subjected to real-time Leishmania qPCR, including an internal reference. Along with other historical and clinical data, living conditions and sampling season were recorded.
Results: All specimens showed perivascular-to-interstitial, predominantly mastocytic, and to a lesser extent eosinophilicneutrophilic inflammatory infiltrate, with moderate-numerous macrophages in 19 samples. Immunostaining and PCR were all Leishmania-negative. Eighteen cats lived in Central-Southern Italy, 23 had outdoor access and 15 were sampled in Spring-Summer.
Conclusion: Despite lifestyle potentially associated with infection, Leishmania was not detected in skin lesions and parasites didn’t seem to play a contributory role to head and neck ulcers in studied cats
Placche acrocordonose nei cani di razza bulldog inglese: studio di epidemiologia descrittiva e aspetti clinici e istopatologici.
No full textScopo del lavoro - Il termine placche acrocordonose (PA) è stato utilizzato per la prima volta da Bidaut et al. nel 2003 in duebulldog e in un carlino per indicare l’insieme di numerose formazioni cutanee peduncolate, lunghe e sottili, raggruppate sul col-lo e/o sul tronco dorsale e caratterizzate istologicamente dalla presenza di formazioni polipoidi contigue costituite da fibre collagene e rivestite da un’epidermide da normale a moderatamente iperplastica. La descrizione dei casi fornita da Bidaut et al. rappresenta l’unico riferimento bibliografico. L’obiettivo di questo lavoro è divalutare la prevalenza di queste lesioni in un database di diagnostica istopatologica, determinare se esista una predisposizionedi razza e approfondire gli aspetti clinici e istopatologici nel bulldog inglese.
Materiali e metodi - Tra il 2004 e il 2015 sono stati esaminati 25890 casi per diagnostica istopatologica nella specie canina e14446 erano rappresentati da biopsie cutanee. In 36 cani, dei quali erano noti età, sesso, razza, sede anatomica e dimensioni della lesione, è stata diagnosticata una lesione acrocordonosa. La predisposizione di razza allo sviluppo della PA è stata calcolata,mediante il Test del Chi quadrato, comparando il numero di cani con PA con il numero di soggetti che contribuivano alla popolazione del registro. Su 12 casi di PA nel bulldog inglese, sono stati valutati i seguenti parametri: aspetto clinico, caratteristiche istopatologiche, presenza o meno di flogosi. Biopsie di cute sana dal collo di 5 bulldog inglesi che non presentavano lesioni der-matologiche, sono stati utilizzati come campioni di controllo.
Risultati - Le PA sono state diagnosticate in 7 diverse razze (bulldog, boxer, carlino, chihuahua, galgo, meticcio, pechinese) conuna maggior frequenza nel bulldog inglese e francese. Le razze predisposte sono risultate essere il bulldog (P<0,01) e il carlino(P<0,05). In 12 dei cani di razza bulldog inglese con PA l’età media alla presentazione era di 45,3 mesi e si trattava di soggetti maschi. Clinicamente le lesioni si caratterizzavano per la presenza di aree singole o multifocali ricoperte di escrescenze confluenti e variabilmente peduncolate. In alcune PA si osservavano alopecia, eritema, iperpigmentazione, scaglie e croste. Le stesse lesioni erano presenti anche nella cute circostante le PA in 11/12 dei cani di razza bulldog inglese studiati, affetti da malattie cutanee di diversa natura. Le sedi coinvolte sono state il collo (4/12), il tronco dorsale (7/12) o entrambe (1/12). In 11/12 casi venivano segnalati problemi cutanei in altre sedi, incluse altre aree del tronco, le estremità e i padiglioni auricolari. All’istopatologia sono state osservate estroflessioni multiple di aspetto filiforme, cupoliforme o piriforme, rivestite da epidermide moderatamente iperplastica e con un core di connettivo lasso con plesso vascolare prominente e aumento della cellularità perivascolare. Tra le cellule infiammatorie erano presenti mastociti, linfociti, plasmacellule e granulociti. In alcune lesioni l’iperplasia epi-dermica era marcata, accompagnata da fenomeni di esocitosi e formazione di pustole. L’esame dei campioni di controllo (cutedi aspetto normale) ha mostrato la presenza di piccole lesioni riconducibili a PA in stadio precoce in 3/5 casi accompagnata daminima o moderata infiltrazione infiammatoria a prevalenza mastocitaria con prominenza vascolare.
Conclusioni - Il lavoro conferma la predisposizione allo sviluppo delle PA nelle due razze brachicefale, il bulldog e il carlino,come segnalato nella prima descrizione della malattia. Nei 12 casi descritti si trattava di maschi e un confronto tra i casi descritti e altri casi presenti nel database ha fatto emergere come le PA insorgano più frequentemente nei maschi (femmine con o senza queste lesioni erano presenti nell’archivio). Nella maggior parte dei casi (11/12) venivano segnalati problemi cutanei anchein altre sedi, suggerendo che le PA possano rappresentare un modello di reazione clinica e istopatologica osservabile nella cutedorsale di cani di razza bulldog, inglese e francese, e carlino. La presenza di piccole lesioni acrocordonose e minimo infiltratoinfiammatorio in cani clinicamente sani e arruolati come campioni di controllo merita ulteriori approfondimenti
A retrospective histopathological, immunohistochemical and molecular study of the presence of Leishmania spp. in the skin of cats with head and neck ulcerative dermatitis
No full textBACKGROUND:
Head and neck ulcers in cats can arise from allergic and nonallergic disorders, including feline leishmaniosis (FeL). It is important to rule out this aetiological agent in regions that are endemic for canine leishmaniosis, because the drugs used to treat immune-mediated disorders of cats can be contraindicated in the setting of infection.
HYPOTHESIS/OBJECTIVES:
The aim of this study was to evaluate the skin of cats with ulcerative dermatitis of the head or neck for evidence of Leishmania infection using combined immunohistochemistry (IHC) and Polymerase Chain Reaction (PCR). An IHC for tissue histiocytes was also utilized because leishmaniosis may provoke a histiocytic inflammatory response.
ANIMALS:
Twenty seven cats with head and/or neck ulcers.
METHODS:
Skin biopsy specimens were examined for the presence of Leishmania spp. by routine histopathological evaluation and IHC using a polyclonal anti-Leishmania antibody, and by quantitative PCR (qPCR). The ionized calcium-binding adapter molecule-1 (IBA-1) antibody was used to immunolocalize histiocytes. Selected history and clinical data were recorded.
RESULTS:
All specimens showed a superficial mid-perivascular mixed inflammatory infiltrate. The presence of histiocytes was confirmed in 23 of 27 cases with the IBA-1 antibody. Immunohistochemistry and qPCR techniques confirmed the absence of Leishmania in all cases.
CONCLUSIONS AND CLINICAL IMPORTANCE:
Leishmania did not seem to play a role in the pathogenesis of feline ulcerative dermatitis of the head and neck in the subjects studied, despite a lifestyle potentially associated with infection. Histiocytic infiltration of tissue is not a specific marker for Leishmania infection in this population
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
- …
