1,888,132 research outputs found
CAP256 gp140-FL-IP protein vaccine design and characterisation.
No full text(A) Schematic representation of wild type Env and truncated CAP256 gp140-FL-IP (soluble CAP256 Env) in which the native signal peptide is replaced with the human tissue plasminogen activator (TPA) sequence, the furin cleavage site with a flexible linker (FL) sequence and an I559P mutation is introduced. A protein band around 720 kDA, the molecular weight of Env trimers, was observed by Coomassie staining (B) and anti-Env western blotting (C) of NativePAGE protein gels in both the eluate (lane 1) and concentrated eluate (lane 2) after lectin purification (indicated with ***) of CAP256 gp140-FL-IP. Some Env monomers, as defined by molecular weight (MW), were observed in the concentrated eluate (*). (D) The protein profile of CAP256 gp140-FL-IP-His differed when analysed by Coomassie stained NativePAGE protein gels with a much larger proportion of Env MW monomers compared to MW trimers and the appearance of Env MW dimers (indicated with **). Molecular weight was estimated using NativeMark Unstained Protein Standard.</p
FL-SMN and a-SMN knockdown: Multi-neurite neurons.
No full text(A1-B3) Confocal images of hippocampal neurons co-transfected with control or a-SMN or FL-SMN-specific siRNAs together with Venus plasmid (green, A1-A3), fixed after 3DIV and labeled with the anti-a-SMN #553 (red, B1-B2) or anti-FL-SMN antibodies (red, B3). After selective a-SMN or FL-SMN silencing, a significant fraction of hippocampal neurons showed several processes with similar length and no clear evidence of axonal polarization. (C) Percentages of Venus+ hippocampal neurons displaying multi-neuritc morphology after transfection with control (white bars), FL-SMN SiRNA (grey bars) or a-SMN-specific siRNAs (red bars). Data are presented as mean ± SEM of three different experiments of each group (* p < 0.05; ** p < 0.01; n.s.: not significant). Statistical analysis were performed by means of one-way ANOVA followed by Tukey HSD as post hoc comparison test. Scale bar: 10 μm.</p
Il-forma ortografika u t-tifsira semantika fl-għażla tal-lessiku Malti fl-oqsma tat-traduzzjoni u tat-terminoloġija
Get PDFDin l-analiżi turi kif is-Semitiku, ir-Rumanz u l-Ingliż li minnhom magħmul il-Malti
jippermettu dinamiżmu u flessibilità kbira fil-qasam tat-traduzzjoni kemm letterarja kif
ukoll nonletterarja. Il-forma ortografika u t-tifsira semantika tal-kliem Malti-Rumanz huma
ħafna drabi l-istess bħal dawk tal-kliem Malti-Ingliż u dan għax kemm l-Ingliż kif ukoll
it-Taljan għandhom fil-lessiku għadd ta’ għeruq komuni li etimoloġikament imorru lura
għal-Latin Medjevali. Ir-rwol tat-traduzzjoni hu li jwassal it-test sors b’mod awtentiku lillqarrej
tat-test mira u mhux li jippreserva l-element Semitiku fil-Malti. Għalhekk għandha
ssir distinzjoni bejn lingwa u traduzzjoni sabiex jiġi osservat id-determiniżmu lingwisitku
stabbilit mill-awtur tal-lingwa sors fl-għażla tal-lessiku li twassal għar-replika vera tat-test
oriġinali fil-lingwa mira tat-traduttur.peer-reviewe
Investigating the benefits of Family learning (FL) with parents
Get PDFThis study investigates the benefits of Family Learning (FL) with parents. It seeks to establish how FL can be used as a tool to address poverty, promote health and provide quality education for all
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Analysis of unspliced (FL) and singly spliced mRNAs in wt and mutant HIV-1 particles
No full text<p><b>Copyright information:</b></p><p>Taken from "HIV controls the selective packaging of genomic, spliced viral and cellular RNAs into virions through different mechanisms"</p><p></p><p>Nucleic Acids Research 2007;35(8):2695-2704.</p><p>Published online 10 Apr 2007</p><p>PMCID:PMC1885669.</p><p>© 2007 The Author(s)</p> Singly spliced () and FL () RNAs were detected in RNA samples extracted from wt (lanes 1 and 4), ΔSL1SL3 (lanes 2 and 5) and mock (lanes 3 and 6) transfected cells and in corresponding viral particles (lanes 4–6) using standard RT-PCR. As a control, amplification was also performed using pNL4.3 plasmid as a template (lane 8). Bands corresponding to vif-2, vpr-3 and env-1 mRNAs are indicated by arrows. In the mutant virus, all amplicons were shortened by 34 nt, due to the SL1 deletion. This result is representative of at least three independent experiments
FL-SEC: Privacy-Preserving Decentralized Federated Learning Using SignSGD for the Internet of Artificially Intelligent Things
No full textFL-SEC: Privacy-Preserving Decentralized Federated Learning Using SignSGD for the Internet of Artificially Intelligent Thing
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
- …
