1,721,003 research outputs found
L'âge des métacalcaires de l'Unité du Frido (région calabro-lucanienne, Italie) et ses implications géodynamiques.
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Spontaneous low-frequency voltage oscillations in frog saccular hair cells.
No full textSpontaneous membrane voltage oscillations were found in 27 of 130 isolated frog saccular hair cells. Voltage oscillations had a mean peak-to-peak amplitude of 23 mV and a mean oscillatory frequency of 4.6 Hz. When compared with non-oscillatory cells, oscillatory cells had significantly greater hyperpolarization-activated and lower depolarization-activated current densities. Two components, the hyperpolarization-activated cation current, I(h), and the K(+)-selective inward-rectifier current, I(K1), contributed to the hyperpolarization-activated current, as assessed by the use of the I(K1)-selective inhibitor Ba(2+) and the I(h)-selective inhibitor ZD-7288. Five depolarization-activated currents were present in these cells (transient I(BK), sustained I(BK), I(DRK), I(A), and I(Ca)), and all were found to have significantly lower densities in oscillatory cells than in non-oscillatory cells (revealed by using TEA to block I(BK), 4-AP to block I(DRK), and prepulses at different voltages to isolate I(A)). Bath application of either Ba(2+) or ZD-7288 suppressed spontaneous voltage oscillations, indicating that I(h) and I(K1) are required for generating this activity. On the contrary, TEA or Cd(2+) did not inhibit this activity, suggesting that I(BK) and I(Ca) do not contribute. A mathematical model has been developed to test the interpretation derived from the pharmacological and biophysical data. This model indicates that spontaneous voltage oscillations can be generated when the electrophysiological features of oscillatory cells are used. The oscillatory behaviour is principally driven by the activity of I(K1) and I(h), with I(A) playing a modulatory role. In addition, the model indicates that the high densities of depolarization-activated currents expressed by non-oscillatory cells help to stabilize the resting membrane potential, thus preventing the spontaneous oscillations
Some remarks about the Calabria-Peloritani Arc and its relationships with the Southern Apennines
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Frog saccular hair cells dissociated with protease VIII exhibit inactivating BK currents, K(V) currents, and low-frequency electrical resonance.
No full textOutward K currents and electrical resonance of frog (Rana esculenta) saccular hair cells isolated enzymatically with bacterial protease VIII were investigated using the perforated patch-clamp method. Under voltage-clamp conditions we identified two K currents, a voltage-dependent K (K(V)) current, and a partially inactivating iberiotoxin-sensitive K (BK) current. The K(V) current activated at a membrane potential of approximately -50 mV (from a holding potential of -70 mV). Its activation rate was rather slow, having a time constant in the range 5-8 ms at 0 mV. The K(V) current was resistant to tetraethylammonium (10 mM), but was inhibited by 4-aminopyridine (1 mM). A striking feature of the BK current was its inactivation; this was monoexponential and had fast kinetics (tau(inact)=2.7 ms +/-1.2, at -10 mV; n=8). Inactivation of the current was incomplete, a residual sustained component remaining. This varied considerably among hair cells (mean ratio between peak transient and sustained component was 1.22+/-0.18, range 0.53-1.8; n=8). In current-clamp mode steady depolarizing current pulses evoked membrane potential oscillatory responses, with mean frequencies varying between 30 and 100 Hz for membrane potentials from -60 to -40 mV (n=18). Most hair cells (14/18) exhibited damped oscillations, and in the remainder a few initial damped oscillations were succeeded by smaller, undamped voltage oscillations. The peak quality factor and the characteristic frequency assessed on 14 cells displaying only damped oscillatory responses were 2.4+/-1.3 and 59+/-39 Hz, respectively. In contrast, papain-dissociated frog saccular hair cells possess solely a sustained BK current, and exhibited significantly higher resonant frequencies and quality factors. In conclusion, the K currents and the electrical resonance of hair cells dissociated in protease VIII differ markedly from those dissociated with papain, but are similar to those reported for in situ preparations, suggesting that our dissociation procedure preserves the electrophysiological profile of in situ frog saccular hair cells
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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