1,720,966 research outputs found
Differences in marine megafauna in vitro sensitivity highlights the need for species-specific chemical risk assessments
No full textSea turtles, dolphins and dugongs can be exposed to large mixtures of contaminants due to the proximity of foraging locations to anthropogenic inputs. Differences in accumulation and effect result in differences of chemical risk to these species. However, little is known about the effect of contaminants in marine wildlife. Cell-based, or in vitro, exposure experiments offer an ethical alternative to investigate the effect of contaminants in wildlife. Data from in vitro studies can then be placed in an environmental context, by using screening risk assessments, comparing effect data with accumulation data from the literature, to identify risk to populations of marine wildlife. Cytotoxicity of Cr6+, Cd2+, Hg2+, 4,4’-DDE, and PFNA were investigated in primary skin fibroblasts of green turtles, loggerhead turtles, hawksbill turtles, dugongs, Burrunan dolphins, and common bottlenose dolphins. The general order of toxicity for all species was Hg2+> Cr6+ > Cd2+> 4,4′-DDE > PFNA, and significant differences in cytotoxicity were found between species for Cr6+, Cd2+ and PFNA. For Cd2+, in particular, cells from turtle species were less sensitive than mammalian species, and dugong cells were by far the most sensitive. The results from the cytotoxicity assay were then used in combination with published data on tissue contaminant concentrations to calculate risk quotients for identifying populations of each species most at risk from these chemicals. Cr, Cd and Hg were identified as posing risk in all six species. Dugongs were particularly at risk from Cd accumulation and dolphin species were particularly at risk from Hg accumulation. These results demonstrate the importance of using species-specific effect and accumulation data for developing chemical risk assessments and can be used to inform managers of priority contaminants, species, or populations. Development of additional in vitro endpoints, and improving links between in vitro and in vivo effects, would further improve this approach to understanding chemical risk in marine megafauna.No Full Tex
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Temporal changes in chemical contamination of green turtles (Chelonia mydas) foraging in a heavily industrialised seaport
No full textPort Curtis, a major shipping port, has undergone significant expansion in the last decade, with plans for further development into the future. These activities may result in an increase of contaminant concentrations, threatening local wildlife including sea turtles. This study used a species-specific in vitro bioassay to examine spatial and temporal differences in exposure to, and effects of, organic contaminants in green sea turtles foraging in Port Curtis. Blood was collected from 134 green sea turtles (Chelonia mydas) from five locations in the port over four years. Organic contaminants were extracted from blood, and the cytotoxicity of the extracts to primary green sea turtle cells was assessed. Results indicated spatially similar chemical contamination throughout Port Curtis, at levels significant to sea turtle health, and with signs that chemical contamination may be increasing over time. These results can provide valuable information on the health of green turtles as further development occurs.Full Tex
Systematic review of reptile reproductive toxicology to inform future research directions on endangered or threatened species, such as sea turtles
No full textThreatened or endangered reptiles, such as sea turtles, are generally understudied within the field of wildlife toxicology, with even fewer studies on how contaminants affect threatened species reproduction. This paper aimed to better inform threatened species conservation by systematically and quantitatively reviewing available research on the reproductive toxicology of all reptiles, threatened and non-threatened. This review found 178 studies that matched our search criteria. These papers were categorized into location conducted, taxa studied, species studied, effects found, and chemicals investigated. The most studied taxa were turtles (n = 87 studies, 49%), alligators/crocodiles (n = 54, 30%), and lizards (n = 37, 21%). Maternal transfer, sex steroid alterations, sex reversal, altered sexual development, developmental abnormalities, and egg contamination were the most common effects found across all reptile taxa, providing guidance for avenues of research into threatened species. Maternal transfer of contaminants was found across all taxa, and taking into account the foraging behavior of sea turtles, could help elucidate differences in maternal transfer seen at nesting beaches. Sex steroid alterations were a common effect found with contaminant exposure, indicating the potential to use sex steroids as biomarkers along with traditional biomarkers such as vitellogenin. Sex reversal through chemical exposure was commonly found among species that exhibit temperature dependent sex determination, indicating the potential for both environmental pollution and climate change to disrupt population dynamics of many reptile species, including sea turtles. Few studies used in vitro, DNA, or molecular methodologies, indicating the need for more research using high-throughput, non-invasive, and cost-effective tools for threatened species research. The prevalence of developmental abnormalities and altered sexual development and function indicates the need to further study how anthropogenic pollutants affect reproductive output in threatened reptiles.No Full Tex
Validation of an in vitro bioassay using C6/36 insect cells as a model for evaluating toxicity of aquatic contaminants to invertebrates
No full textAquatic invertebrates play an important role in ecosystem functioning and are commonly used for in vivo toxicity assessments of environmental contaminants. However, in vitro bioassays employing invertebrate cell lines remain underutilised, despite their potential to improve toxicity testing and reduce reliance on whole-organism assays. This study aimed to develop and validate a suite of in vitro bioassays using the Asian tiger mosquito (Aedes albopictus) larval cell line (C6/36) to assess the cytotoxicity, oxidative stress, and genotoxicity of 24 common environmental contaminants. Cytotoxic effects were observed in 71 % of tested chemicals, while 25 % induced oxidative stress, and 63 % showed measurable genotoxicity. A strong correlation (R² = 0.83) between in vitro cytotoxicity data and acute toxicity data previously reported for Daphnia spp. suggests that C6/36 cells may serve as a reliable surrogate for traditional in vivo invertebrate toxicity tests. These findings support the broader application of invertebrate cell lines for environmental risk assessment, offering a refined approach for evaluating the toxicological properties of aquatic contaminants, while potentially reducing reliance on animal testing.Full Tex
Development and application of species-specific cell-based bioassays to assess toxicity in green sea turtles
No full textDespite the detection of a wide range of contaminants in the blood of green turtle populations foraging in three locations of northern Queensland – Upstart Bay, Cleveland Bay and the Howick Group of Reefs, little is known about the effects of these contaminants on turtle health. Newly developed cell-based bioassays using green turtle primary cell cultures provide an ethical, reproducible, and high-throughput method for assessing the risk of chemical exposure sea turtles. In this project, the toxicity of six priority metals (Mn, Co, Mo, As, Sb, Cu) and blood extracts from foraging turtles were tested in two bioassays adapted to green turtle primary skin and liver cells. Cytotoxicity of metals and blood extracts was measured in primary skin fibroblast cells using a resazurin assay. Glutathione-S-transferase (GST) activity was measured in primary skin fibroblasts and primary liver epithelial cells following exposure to metals and blood extracts. Arsenic, molybdenum, cobalt and copper were found to be cytotoxic to green turtle skin cells. Only manganese, cobalt and copper were found to alter GST activity, predominantly in skin cells, indicating a higher sensitivity of green turtle skin cells compared to liver cells. Effect concentrations of metals in both bioassays were above concentrations found in turtle blood. Turtle blood extracts from the three foraging grounds showed differences in cytotoxicity and GST activity. In both assays, blood extracts of turtles from Upstart Bay were the most toxic, followed by those from Cleveland Bay, then the Howick Reefs, suggesting turtles from Upstart Bay and Cleveland Bay may be at risk from current concentrations of organic contaminants. This study demonstrates that species-specific cell-based bioassays can be used effectively to assess chemical risk in sea turtles and their foraging grounds, and could be applied to assess chemical risk in other marine wildlife.No Full Tex
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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