182,125 research outputs found
Hsc70 is a binding partner of FILIP.
No full text(A) An SDS-PAGE gel image of the co-purified proteins with a partial fragment of FILIP including the Herpes_BLLF1 domain fused to glutathione S-transferase. (B) FILIP binds to Hsc70 in vivo. Protein lysates were obtained from the adult mouse heart. Immunoprecipitation was performed using an anti-FILIP antibody, and blots were probed with an anti-Hsc70 antibody. (C) The Herpes_BLLF1 domain of FILIP is responsible for binding to Hsc70. The indicated vectors were transfected into COS-7 cells. Immunoprecipitation was performed using an anti-FLAG antibody, and blots were probed using an anti-Hsc70 antibody. IgG, normal mouse IgG as a negative control.</p
The Difference Between the Optative and the “Modal” Indicative in Homeric Greek: Four Case Studies – Part 2: The Indicative and the εἰ Μή-Clauses
Get PDFIn epic Greek both the optative and the indicative (the so-called “modal indicative”) can be used in contexts where the degree of realization is uncertain or even impossible, while in Attic Greek only the indicative is used. In these two articles I discuss whether there is a difference between the optative and the modal indicative in these contexts and/or if it can be determined which was the original mood. As there are about 1500 optatives and 250 modal indicatives in Homer, it is not possible to discuss them all and, therefore, I focus on the passages in which aorist forms of γιγνώσκω, βάλλω and of ἴδον appear, and those conditional constructions in the Odyssey in which the postposed conditional clause is introduced by εἰ μή with either a “modal” indicative or optative. The corpus comprises 100 forms (80 optatives and 20 indicatives), but in each example I also address the other modal indicatives and optatives in the passages, which adds another 50 forms to the corpus. In this part (part 2) I address the modal indicatives, and discuss the postposed conditional clauses introduced by εἰ μή in the Odyssey, both in the indicative and the optative. Subsequently I analyze several instances in which the interpretation depends on the viewpoint of the hearer and the speakers, as what is possible for a speaker might be impossible for the hearer and vice versa. When comparing the data relating to the optative and the indicative, and especially that of the postposed conditional clauses introduced by εἰ μή, it can be noted that the indicative has more frequently an exclusively past reference and that it is more often genuinely unreal than 302 FILIP DE DECKER the optative, which often combines the notion of the possible, remotely possible and unreal. In my opinion this clearly indicates that the indicative eventually prevailed and replaced the optative because of the past reference
3D beam-column finite elements under tri-axial stess-strain states: non-uniform shear stress distribution and warping
Get PDFIn many engineering structures, the effects of shear and torsional loads are an important aspect of both the analysis and the design process. These effects are usually neglected in typical framed structures. However, in some relevant cases, such as bridges, shear walls or thin-walled frames, it is essential to account for the shear and torsional loads and their interaction with the other loading conditions to correctly reproduce the structural response.
In this framework, the main task is to accurately describe the nonlinear structural response in terms of global behavior and local stress-strain distributions, reproducing the coupling of the stress components and its influence on the global response. This results even more important in large scale structures made of cementitious and/or innovative composite materials, widely adopted in nowadays professional practice. Indeed, these structures usually show degrading mechanisms and softening behavior. Hence, they require sophisticated computational models and ad hoc analysis strategies to predict
the structure capacity under severe loading conditions.
A standard approach to analyze these structures is the adoption of beam-column finite element (FE) models, which are often preferred with respect to two-dimensional (2D) plate/shell or three-dimensional (3D) FEs, because of their efficiency and low computational cost. However, most beam-column FE formulations are based on theclassical Euler-Bernoulli or Timoshenko theory, assuming the cross-sections to remain plane during the loading process. This assumption requires specific corrective measures, when the shear and torsion and the related warping effects are pronounced.
This work discusses the simulation of RC members with a 3D 2-node beam FE that includes warping effects. The FE formulation in [1] is extended to allow the description of structural members with softening material behavior. The governing equations are derived from a four-field Hu-Washizu variational principle, with independent interpolation
of the warping displacement field from the rigid section displacements, the generalized section deformations and the material stress fields. In particular, the warping of the cross-section is described by interpolating the out-of-plane displacement with
the addition of a variable number of local degrees of freedom to those commonly used for the beam FE. The global nonlinear response and the local distributions of strains and stresses are described introducing a fiber cross-section discretization. Hence, the coupling of axial, flexural, shear and torsional effects in terms of material response is automatically taken into account.
Focusing on RC structures, the damaging mechanisms of the concrete material is described by adopting a new 3D nonlinear constitutive relationship with plasticity and damage. This is an enhanced version of that proposed in [2] and introduces the description of the unilateral effects typically appearing in concrete-like materials, due to the crack opening and closure. A Drucker-Prager type plastic model is coupled with a two-parameter isotropic damage model, where two scalar variables are used to describe the damage in tension and compression, respectively.
The localization problems and the related mesh-dependency, due to the softening material behavior, are controlled through a regularization technique based on a properly modified nonlocal integral procedure. For beam-column FEs, the nonlocal strain measures are evaluated performing the integration of the local generalized section deformations along the element axis, whereas for 2D FEs the nonlocal integration is performed considering the generalized membrane/plate deformations.
The proposed model is implemented and validated through some correlation studies. These consider the numerical analysis of a series of plain concrete and RC beams subjected to torsional loads and of two RC shear walls. The results are compared with experimental measurements and with those of standard FE beam models
The regulation of spine length by FILIP in primary neurons requires Hsc70.
No full text(A) Treatment with clofibric acid resulted in shorter spines in piriform neurons. Scale bar = 2 μm. The graph shows summary data from 554 spines from 6 neurons treated with vehicle and 571 spines from 4 neurons treated with clofibric acid. *p < 0.01 (B) Expression of FILIP d872-1111 in hippocampal neurons did not result in elongated spines. Scale bar = 1 μm. The graph shows summary data from 882 spines from 5 neurons (control), 851 spines from 5 neurons (FILIP d872-1111), and 696 spines from 5 neurons (FILIP). *p < 0.01 (C) Inhibition of the function of Hsc70 reverses the effects of FILIP on spine length. Scale bar = 1 μm. The graph shows summary data from 863 spines from 6 neurons (control/vehicle), 821 spines from 6 neurons (control/VER-155008), 603 spines from 6 neurons (FILIP/vehicle), and 741 spines from 6 neurons (FILIP/VER-155008). **p < 0.01, *p < 0.05.</p
Exogenous expression of FILIP influences the subcellular distribution of non-muscle myosin IIb.
No full text(A) Non-muscle myosin IIb was visualized (red) using an anti-NMHCIIb antibody in COS-7 cells. Green, bicistronic GFP expression vector for FILIP; blue, cell nuclei labeled with Hoescht dye. Scale bar = 10 μm. (B) The graph shows the ratio of the cells exhibiting a stress fiber-like distribution of non-muscle myosin IIb. The numbers of cells containing stress fiber-like distributions of non-muscle myosin IIb/total cells were: 287/330 control cells, 74/318 FILIP-expressing cells, 58/312 FILIP d248-685-expressing cells, 186/323 FILIP d872-1111-expressing cells, and 195/310 FILIP d687-960-expressing cells. *p < 0.01 (Fisher’s exact test) (C) The graph shows the effects of jasplakinolide. The numbers of cells containing a stress fiber-like distribution of non-muscle myosin IIb/total cells were: 285/312 control cells treated with vehicle, 286/314 control cells treated with jasplakinolide, 76/313 FILIP-expressing cells treated with vehicle, and 180/310 FILIP-expressing cells treated with jasplakinolide. *p < 0.01 (Fisher’s exact test).</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
La toponymie de Sv. Filip i Jakov
Get PDFAutori u radu donose toponomastičku građu Sv. Filipa i Jakova, njegovog zaleđa i obalnog prostora između Turnja i Biograda, prikupljenu terenskim istraživanjem. Nakon uvoda u povijest imena i naseljenosti naselja, građa je sistematizirana i analizirana po jezičnim i semantičkim kriterijima. Na kraju rada autori donose iscrpna kazala, referentno i abecedno, i toponomstičke karte istraženoga prostora.Le village de Sv. Filip i Jakov date à peu près de la fin du quinzième siècle. La première attestation écrite du nom du village (…ex opposito ville sancti Philippi Iacobi…) date de 1494. A partir de la fin de la deuxième guerre mondiale, le village est nommé Filipjakov, ce qui est, jusqu’à présent, le seul nom du village figurant dans la communication quotidienne. La toponymie du village et de son arrière pays est très récente, croate dans sa majeure partie, et sans éléments alloglotiques qui proviendraient du substrat grec ou dalmatoroman. Le reste des éléments alloglotiques sont les emprunts vénitiens et, le plus récemment, italiens. Sémantiquement, la toponymie de Sv. Filip i Jakov correspond dans sa plus grande partie à la toponymie de la côte adriatique. Les procédés les plus fréquents de la dénomination sont: a) attribution, b) déscription, c) association fondée sur la similarité (métaphores et onomatopées) et c) association fondée sur la contiguïté (métonymies). Parmi les faits sémantiques surprenants on peut constater une présence très faible des toponymes motivés par des noms des animaux ou des plantes
Mitomycin C in highly myopic eyes - Author reply
No full textOphthalmology. 2005 Feb;112(2):208-18; discussion 219.
Mitomycin C modulation of corneal wound healing after photorefractive keratectomy in highly myopic eyes.
Gambato C, Ghirlando A, Moretto E, Busato F, Midena E.
SourceRefractive Surgery Service and Antimetabolite Therapy Research Unit, Department of Ophthalmology, University of Padova, Padova, Italy.
Abstract
PURPOSE: To evaluate the role of topical mitomycin C in corneal wound healing (CWH) after photorefractive keratectomy (PRK) in highly myopic eyes.
DESIGN: Prospective, double-masked, randomized clinical trial.
PARTICIPANTS: Seventy-two eyes of 36 patients affected by high (>7 diopters) myopia.
METHODS: In each patient, one eye was randomly assigned to PRK with intraoperative topical 0.02% mitomycin C application, and the fellow eye was treated with a placebo. Postoperatively, mitomycin C-treated eyes received artificial tears (3 times daily, tapered in 3 months), whereas the fellow eye was treated with fluorometholone sodium 2% and artificial tears (3 times daily, tapered in 3 months).
MAIN OUTCOME MEASURES: Uncorrected visual acuity (UCVA) and best-corrected visual acuity (BCVA), contrast sensitivity, manifest refraction, and biomicroscopy. Contrast sensitivity was determined using the Pelli-Robson chart. Corneal confocal microscopy documented CWH.
RESULTS: Mean follow-up was 18 months (range, 12-36). No side effects or toxic effects were documented. At 12-month follow-up examination, UCVAs (logarithm of the minimum angle of resolution) were 0.4+/-0.48 and 0.5+/-0.53 (P = .03) in mitomycin C-treated eyes and corticosteroid-treated eyes, respectively. At 1 year, corneal haze developed in 20% of corticosteroid-treated eyes, versus 0% of mitomycin C-treated eyes. At 12, 24, and 36 months, corneal confocal microscopy showed activated keratocytes and extracellular matrix significantly more evident in untreated eyes (Ps = 0.004, 0.024, and 0.046, respectively).
CONCLUSION: Topical intraoperative application of 0.02% mitomycin C can reduce haze formation in highly myopic eyes undergoing PRK.
Comment in
Ophthalmology. 2006 Feb;113(2):357; author reply 357-8
National Co-ordination of the Fight against Trafficking in Human Beings: Recommendations for Poland as a 'New' EU Member State
Get PDFAs a world-wide problem, trafficking in human beings now attracts some well-deserved attention after years of political reservation and operational hesitation in European Union Member States. This working paper examines a particular aspect of the EU policy against this phenomenon: its change vis-à-vis the 2004 enlargement to the East. As the topics, actors and activities on the anti-trafficking scene multiply, national co-ordination of the fight against trafficking in human beings is in need of several improvements. The following ideas derive from research performed in Florence, as well as from consultations with anti-trafficking experts and the study of official documents of international organisations and governments. 'Pooling of resources', together with the creation of a national coordinator, are the key concepts of the envisaged enhanced model of Poland's policy.Poland; immigration policy; asylum policy; cross-border crime; enlargement
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