2,341 research outputs found
S288c
No full textCell-cell and cell-surface adherence represents initial steps in forming multicellular aggregates or in establishing cell-surface interactions. The commonly used Saccharomyces cerevisiae laboratory strain S288c carries a flo8 mutation, and is only able to express the flocculin-encoding genes FLO1 and FLO11, when FLO8 is restored. We show here that the two flocculin genes exhibit differences in regulation to execute distinct functions under various environmental conditions. In contrast to the laboratory strain Sigma 1278b, haploids of the S288c genetic background require FLO1 for cell-cell and cell-substrate adhesion, whereas FLO11 is required for pseudohyphae formation of diploids. In contrast to FLO11, FLO1 repression requires the Sin4p mediator tail component, but is independent of the repressor Sfl1p. FLO1 regulation also differs from FLO11, because it requires neither the KSS1 MAP kinase cascade nor the pathways which lead to the transcription factors Gcn4p or Msn1p. The protein kinase A pathway and the transcription factors Flo8p and Mss11p are the major regulators for FLO1 expression. Therefore, S. cerevisiae is prepared to simultaneously express two genes of its otherwise silenced FLO reservoir resulting in an appropriate cellular surface for different environments
Reptricket. Förord till Lars Gustafsson: Mot noll
No full textIntroduction to a collection of philosophical essays by Swedish author Lars Gustafsson (b. 1936)
Author Functions in Lars Kepler\u27s The Hypnotist: An Analysis
No full textThis paper examines Foucault\u27s notion of the author function as it pertains to Lars Kepler\u27s bestselling 2011 crime thriller, The Hypnotist. Lars Kepler is the pseudonym of a Swedish husband-wife writing duo, making him the perfect subject for analysis centering on illusory notion of the author. This paper will answer these questions: Who is the true author of The Hypnotist? What factors influence the author function of this bestelling novel? And what can The Hypnotist phenomenon tell us about the relationships between authors and their readers? This paper will demonstrate that no literary works may be ascribed to an individual person, and that authors hold no privileged knowledge of the works they produce, because authors cease to be authors the moment pen is lifted from page
The Yeast HtrA Orthologue Ynm3 Is a Protease with Chaperone Activity that Aids Survival Under Heat Stress
No full textYnm3 is the only budding yeast protein possessing a combination of serine protease and postsynaptic density 95/disclarge/zona occludens domains, a defining feature of the high temperature requirement A (HtrA) protein family. The bacterial HtrA/DegP is involved in protective stress response to aid survival at higher temperatures. The role of mammalian mitochondrial HtrA2/Omi in protein quality control is unclear, although loss of its protease activity results in susceptibility toward Parkinson's disease, in which mitochondrial dysfunction and impairment of protein folding and degradation are key pathogenetic features. We studied the role of the budding yeast HtrA, Ynm3, with respect to unfolding stresses. Similar to Escherichia coli DegP, we find that Ynm3 is a dual chaperone-protease. Its proteolytic activity is crucial for cell survival at higher temperature. Ynm3 also exhibits strong general chaperone activity, a novel finding for a eukaryotic HtrA member. We propose that the chaperone activity of Ynm3 may be important to improve the efficiency of proteolysis of aberrant proteins by averting the formation of nonproductive toxic aggregates and presenting them in a soluble state to its protease domain. Suppression studies with Delta ynm3 led to the discovery of chaperone activity in a nucleolar peptidyl-prolyl cis-trans isomerase, Fpr3, which could partly relieve the heat sensitivity of Delta ynm3
HMCLab: a framework for solving diverse geophysical inverse problems using the Hamiltonian Monte Carlo method
No full textThe use of the probabilistic approach to solve inverse problems is becoming
more popular in the geophysical community, thanks to its ability to address
nonlinear forward problems and to provide uncertainty quantification. However,
such strategy is often tailored to specific applications and therefore there is
a lack of a common platform for solving a range of different geophysical
inverse problems and showing potential and pitfalls. We demonstrate a common
framework to solve such inverse problems ranging from, e.g, earthquake source
location to potential field data inversion and seismic tomography. Within this
approach, we can provide probabilities related to certain properties or
structures of the subsurface. Thanks to its ability to address high-dimensional
problems, the Hamiltonian Monte Carlo (HMC) algorithm has emerged as the
state-of-the-art tool for solving geophysical inverse problems within the
probabilistic framework. HMC requires the computation of gradients, which can
be obtained by adjoint methods, making the solution of tomographic problems
ultimately feasible. These results can be obtained with "HMCLab", a tool for
solving a range of different geophysical inverse problems using sampling
methods, focusing in particular on the HMC algorithm. HMCLab consists of a set
of samplers and a set of geophysical forward problems. For each problem its
misfit function and gradient computation are provided and, in addition, a set
of prior models can be combined to inject additional information into the
inverse problem. This allows users to experiment with probabilistic inverse
problems and also address real-world studies. We show how to solve a selected
set of problems within this framework using variants of the HMC algorithm and
analyze the results. HMCLab is provided as an open source package written both
in Python and Julia, welcoming contributions from the community.Comment: 21 pages, 4 figure
”Lärda nyheter” i Peter Hernquists korrespondens till Carl von Linné och Abraham Bäck - med kommentarer och utvikningar
No full textI detta Meddelande nr 55 från Veterinärhistoriska museet har författaren - professor emeritus Lars-Erik Appelgren - gjort ett urval av den korrespondens som Peter Hernquist hade med sina mentorer Carl von Linné och Abraham Bäck under sin vistelse i Frankrike, varvid ”Lärda nyheter” varit en ledstjärna för urvalet. Speciellt har breven till Bäck försetts med författarens personliga kommentarer men även kompletterats med faktaupplysningar från andra källor än breven om berörda nyheter. För att underlätta läsningen har dessa kommentarer omgetts med enkelkonturerad och utvikningarna med dubbelkonturerad ram. Lars-Erik Appelgren har inte bara genom sitt veterinärhistoriska författarskap utan också genom att ställa sina fackliga kunskaper och sin eminenta estetiska läggning till förfogande gjort Veterinärhistoriska museet ovärderliga tjänster. Det är med stor glädje jag noterar att region Uppsala nyligen visat sin uppskattning genom att tilldela Lars-Erik sitt Medicinhistoriska stipendium med motiveringen att de vill ”lyfta fram ett viktigt men ofta förbisett område inom medicinhistorien: veterinärmedicinen”. Med de varmaste gratulationer
Interpreting Wage Bargaining Norms
No full textFrom the mid-1990s onwards, Swedish wage bargaining has been characterised by informal co-ordination of the wage claims of big unions and bargaining cartels. In particular, it has been understood that the manufacturing sector should lead by first agreeing on a pay increase, whereafter the service sector and public sector unions choose a similar increase. We analyse his setup with two possible theoretical interpretations: (i) the manufacturing sector as a tackelberg leader and (ii) a normative role for the manufacturing sector’s pay increase, upported either by unmodelled social pressure or a modeled loss aversion (envy) of the heltered sector unions. The conclusion of the analysis is that the normative or leading role of one sector – in the Swedish case the manufacturing sector – can potentially bring big benefits for employment and output. Generalising an idea suggested by Lars Calmfors and Anna Larsson, our analysis also generates a rudimentary theory of why the wage increase norm sometimes binds and sometimes not. A comparison of the model predictions and the observed outcomes of the last five wage bargaining rounds in Sweden suggests that the model is generally consistent with the empirical observations: wage moderation and norm observance are stronger when the manufacturing industry’s initial relative wage is low.wage bargaining; bargaining co-ordination
Dan Andersson
No full textSpecial feature issue on the Swedish author Dan Andersson, with articles by Anders Hallengren, Nisse Munck, Lars Furuland, Lars Järnemo (2), Gösta Ågren, Inge Mattsson, Arne Säll, and Bure Holmbäck.</p
Representation of female characters in the films of Lars von Trier
No full textOslanjajući se na sličnosti u ženskim likovima Lars Von Triera, detektirani su patrijarhalni i religijski obrasci koji su okosnica radnje filmova Lomeći valove, Dogville i Antikrist. Kroz religijsku i patrijarhalnu optiku autor čita Lars Von Triera kao redatelja koji istu optiku kritizira i preispituje u svom stvaralaštvu.Relying on the similarities in Lars von Trier's female characters, patriarchal and religious patterns have been detected that are the backbone of the plot of the films Breaking the Waves, Dogville and the Antichrist. Through religious and patriarchal optics, the author reads Lars Von Trier as a director who criticizes and questions the same optics in his work
Representation of female characters in the films of Lars von Trier
No full textOslanjajući se na sličnosti u ženskim likovima Lars Von Triera, detektirani su patrijarhalni i religijski obrasci koji su okosnica radnje filmova Lomeći valove, Dogville i Antikrist. Kroz religijsku i patrijarhalnu optiku autor čita Lars Von Triera kao redatelja koji istu optiku kritizira i preispituje u svom stvaralaštvu.Relying on the similarities in Lars von Trier's female characters, patriarchal and religious patterns have been detected that are the backbone of the plot of the films Breaking the Waves, Dogville and the Antichrist. Through religious and patriarchal optics, the author reads Lars Von Trier as a director who criticizes and questions the same optics in his work
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