122 research outputs found
Dutiful Justice (book review)
No full textSheldon Novick’s biography, Honorable Justice: The Life of Oliver Wendell Holmes, is a traditional biography of one of the most important public figures in the United States since the Civil War.
Although the author disclaims it, Honorable Justice is a defense of Holmes. Novick writes of some of Holmes’ faults, but too often Holmes’ human imperfections are defended as strengths. It appears that Novick was trying hard to defend Holmes from late twentieth century critiques. This defense of Holmes seems a misguided attempt to re(de)ify Holmes to a group of readers which will likely include a large proportion of skeptical, professional ironists.
Holmes was a complex man whose life is worth the effort Novick clearly expended on the biography. The complexity of Holmes is pursued on enough occasions that the book is worthwhile reading, and for that Novick should be commended. However, it remains uncertain whether Holmes was an honorable man, or an Honorable Justice
Evolutionarily assembled cis-regulatory module at a human ciliopathy locus.
No full textNeighboring genes are often coordinately expressed within cis-regulatory modules, but evidence that nonparalogous genes share functions in mammals is lacking. Here, we report that mutation of either TMEM138 or TMEM216 causes a phenotypically indistinguishable human ciliopathy, Joubert syndrome. Despite a lack of sequence homology, the genes are aligned in a head-to-tail configuration and joined by chromosomal rearrangement at the amphibian-to-reptile evolutionary transition. Expression of the two genes is mediated by a conserved regulatory element in the noncoding intergenic region. Coordinated expression is important for their interdependent cellular role in vesicular transport to primary cilia. Hence, during vertebrate evolution of genes involved in ciliogenesis, nonparalogous genes were arranged to a functional gene cluster with shared regulatory elements
Identification of yeast component A: reconstitution of the geranylgeranyltransferase that modifies Ypt1p and Sec4p.
No full text
ACKNOWLEDGMENTS The author would like to convey his appreciation to his supervisory committee
No full textArroyo) for their support and guidance. Special thanks go to Dr. Crane who gave the author the opportunity to work on the autonomous vehicle project and continually provided important insight and advice. This work would not have been possible without the support of the Air Force Research Laboratory at Tyndall Air Force Base, Florida. Thanks go to Al Neese and the rest of his staff. The author’s work presented in this dissertation focuses on only part of the tasks required for autonomous navigation. Other project members have addressed the remaining tasks. Therefore, thanks go to those who have worked on the autonomous navigation project, both past and present, at the Center for Intelligent Machines and Robotics. Individual thanks go to the project manager, David Armstrong, for his invaluable input, and to office mate David Novick, for his unending programming advice. Finally, special thanks go to the author’s wife, Jennifer Lisa Wit, who provided continuous encouragement and inspiration needed to finish this dissertation. ii
Characterization of new mutants in the early part of the yeast secretory pathway isolated by a [3H]mannose suicide selection.
No full textOsmohomeostasis and vacuole biogenesis genes in the yeast saccharomyces cerevisiae
No full textThe putative role of the S. cerevisiae vacuole in osmohomeostasis, as well as its biogenesis was analysed by taking a mutational approach. 97 mutants unable to tolerate high concentrations of salt were isolated and examined for aberrant vacuolar phenotypes. A comprehensive phenotypic analysis was able to demonstrate that apart from osmosensitivity most mutations conferred other properties such as altered vacuolar morphology, the inability to perform gluconeogenesis and/or the mislocalization of vacuolar proteins to the cell surface. The mutants fall into at least 20 complementation groups, termed ssv for salt sensitive vacuolar mutants, of which 3 genetically overlap with complementation groups isolated by others. This analysis provides evidence that in 5. cerevisiae correct vacuolar biogenesis is required for osmotolerance as well as other important cellular processes. To elucidate vacuolar osmohomeostasis at the molecular level, one gene, SSV7, was cloned from a genomic DNA library by complementation of a ssv7-l mutation and its sequence determined. It encodes a novel 927 amino acid protein with limited structural homology to the functional domains of two nucleotide exchange factors from 5. cerevisiae, namely CDC25 and BUD5. A mutation in the SSV7 gene confers a pleiotropic phenotype including fragmented vacuoles and the absence of a nucleus. The mutation apparently uncouples mitosis from cell growth, which results in an increased cell size of a ssv7-l mutant. The role of SSV7 in the general concept of vacuole biogenesis is discussed, as is the observed fact that a number of mutants in intermediate biosynthetic pathways confer a vacuolar protein sorting defect
- …
