177,676 research outputs found
IsotopicLabelling: an R package for the analysis of MS isotopic patterns of labelled analytes
No full textMotivation: Labelling experiments in biology usually make use of isotopically-enriched substrates, with the two most commonly employed isotopes for metabolism being 2 H and 13 C. At the end of the experiment some metabolites will have incorporated the labelling isotope, to a degree that depends on the metabolic turnover. In order to propose a meaningful biological interpretation, it is necessary to estimate the amount of labelling, and one possible route is to exploit the fact that MS isotopic patterns reflect the isotopic distributions.
Results: We developed the IsotopicLabelling R package, a tool able to extract and analyse isotopic patterns from liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-MS (GC-MS) data relative to labelling experiments. This package estimates the isotopic abundance of the employed stable isotope (either 2 H or 13 C) within a specified list of analytes.
Availability: The IsotopicLabelling R package is freely available at https://github.com/RuggeroFerrazza/IsotopicLabellin
Il gel piastrinico nel trattamento del “piede diabetico”: esperienza preliminare
No full textIL GEL PIASTRINICO NEL TRATTAMENTO DEL “PIEDE DIABETICO”: ESPERIENZA PRELIMINARE
Massimo Chiaretti*, Giancarlo Ferrazza+, Rita Maria Fracassi@ , Andrea Negro°, Domenico Tuscano*
(*) Dipartimento di Chirurgia Generale, Specialità Chirurgiche e Trapianti d’Organo “Paride Stefanini” Azienda Policlinico Umberto I, Università degli Studi di Roma “La Sapienza”
(+ ) Servizio di Immunoematologia e Medicina Trasfusionale Azienda Policlinico Umberto I, Università degli Studi di Roma “La Sapienza”
(@) Servizio di Immunoematologia IRCCS Ospedale Bambino Gesù, sede di Roma.
(°) Azienda S. Andrea, II Facoltà di Medicina e Chirurgia dell’Università degli Studi di Roma “La Sapienza”
La nostra esperienza evidenzia l’utilità del gel piastrinico (GP) nel trattamento ambulatoriale delle piaghe torpide della pianta del piede in paziente diabetico. Il GP guarisce in tempi prevedibili ulcerazioni torpide che richiedevano lunghissimi periodi di medicazioni complesse senza raggiungere il risultato prefissato. La nostra esperienza, analogamente ai risultati di altri autori, depone favorevolmente per questo trattamento. La metodica personalmente messa a punto prevede il curettage dopo disinfezione, l’inoculazione di 1 UI di insulina pronta alla base del cratere ulcerativo e quindi l’applicazione di 5 ml di GP con 1 ml di Trombina autologa attivata. L’applicazione può essere eseguita mediante un puntale spray o cannula a seconda delle esigenze dell’operatore o del tipo di lesione. In caso di ferite più piccole o man mano che la ferita in trattamento si riduce di ampiezza, il prodotto può essere aliquotato e congelato per successive somministrazioni. Il GP e la trombina vanno spalmati in situ e su un supporto inerte riassorbibile di acido ialuronico (Hialogel) con il quale si zaffa la cavità e che impedisce la dispersione della parte liquida e non completamente gelificabile, ricca dei fattori di crescita (PDGF, TGF, EGF, ed altri). Il rapporto strettamente collaborativo tra dermatologo, chirurgo, ortopedico, immunotrasfusionista, diabetologo e cardiologo, risolve questa problematica con un approccio multidisciplinare. Lo stretto coordinamento tra centro trasfusionale ed ambulatorio chirurgico evita inutili attese al Paziente, accorcia i tempi di esecuzione della medicazione, abbatte i costi per l’impiego di materiali e alla lunga diventa conveniente rispetto al tradizionale trattamento.Our study proves the usefulness of platelet gel in the treatment of the diabetic foot. We started in January 2006 to treat diabetic wounds of the foot in the outpatients’ surgical department with encouraging results. Despite its expensive and complex preparation, the platelet gel is useful and convenient because it succeeds in shortening the ambulatory treatment period. Besides, in our opinion, the multidisciplinary approach of this treatment is rather important: actually, it implies the cooperation of dermatologist, surgeon, orthopaedist, immunologist, diabetologist and, if necessary, the cardiologist. That is why it reduces wastes of work-time and the expenses for consultants, medications and dressing material
Appropriate Similarity Measures for Author Cocitation Analysis
No full textWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
TLC surface integrity affects the detection of alkali adduct ions in TLC-MALDI analysis
No full textDirect coupling of thin-layer chromatography (TLC) with matrix-assisted laser desorption ionization (MALDI) mass spectrometry allows fast and detailed characterization of a large variety of analytes. The use of this technique, however, presents great challenges in semiquantitative applications because of the complex phenomena occurring at the TLC surface. In our laboratory, we recently observed that the ion intensities of several alkali adduct ions were significantly different between the top and interior layer of the TLC plate. This indicates that the integrity of the TLC surface can have an important effect on the reproducibility of TLC- MALDI analyses. Graphical Abstract MALDI imaging reveals that surface integrity affects the detection of alkali adductions in TLC-MALD
"Closing the R&D Gap, Evaluating the Sources of R&D Spending"
No full textBoth spending and tax policies have been implemented in the United States with the goal of stimulating private sector research and development (R&D). Karier questions whether current R&D policy, especially the research and experimentation tax credit, can contribute to closing the gap between nondefense expenditures on R&D in the United States and such expenditures in other countries, such as Japan and Germany. He also explores possible changes to our current R&D policy to make it more effective.
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Letter from R. R. Zellick, Assistant Trust Officer, Anglo California National Bank of San Francisco, to Joseph R. Goodman, October 2, 1942
No full textLetter from R. R. Zellick, Assistant Trust Officer at The Anglo California National Bank of San Francisco, to Joseph R. Goodman, regarding property owned by Dave Tatsuno. Zellick mentions a dispute between current tenants and Tatsuno, and that Tatsuno has asked Goodman to help locate trustworthy tenants.Personal correspondence, organizational records, government documents, publications, and other papers created or collected by Joseph R. Goodman documenting the forced removal and incarceration of Japanese Americans during World War II, as well as organized resistance to incarceration. Included in the collection are records of the Japanese Young Men's Christian Association and the Japanese American Citizens' League in San Francisco, including papers of the Japanese YMCA's executive secretary Lincoln Kanai; Sakai family papers; Goodman's correspondence to and from Japanese American incarcerees, organizations opposing forced removal and incarceration of Japanese Americans, the War Relocation Authority, and others; publications, photographs, and ephemera from the Topaz Relocation Center, where Goodman taught high school; War Relocation Authority records and publications; and newspaper clippings, pamphlets, and reports about forced removal and incarceration created by various government, religious, and civic organizations, in California and nationwide
Drenos de nitrogênio em cultivares da Panicum maximum Jacq.
No full textO conhecimento de como as forrageiras adquirem e utilizam o nitrogênio quando sujeito à desfolhação pode auxiliar na determinação de práticas de manejo e adubação que aumentem a produtividade e reduzam os riscos de impacto ambiental
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