31 research outputs found
Papel de la proteína FliL en el funcionamiento del flagelo de Rhodobacter sphaeroides /
No full text\ua0tesis que para obtener el grado de Doctor en Ciencias Biomédicas, presenta Fernando Suaste Olmos ; asesor Laura Camarena Mejía. 87 páginas :\ua0ilustraciones. Doctorado en Ciencias Biomédicas\ua0UNAM, Instituto de Investigaciones Biomédicas,\ua0201
Conociendo al COVID-19 y la labor odontológica ante la pandemia
No full textEn febrero del presente año la Organización Mundial
de la Salud (OMS) dio a conocer la presencia de una
nueva cepa de un virus que estaba generando enfermedades
respiratorias altamente contagiosas, el COVID-
19, ¿pero qué es este virus? Los coronavirus
(CoVs) son una familia de virus con genoma RNA
pertenecientes al orden Nidovirales, los cuales se han
clasificado en tres subgrupos α, β, y γ, los subtipos “α”
y “β” se encuentran distribuidos en mamíferos, incluyendo
humanos y el subtipo “γ” en aves, sin embargo,
un cuarto grupo denotado como “δ” ha sido reclasificado
debido a su capacidad de infectar porcinos 1; estos
subtipos de coronavirus se caracterizan principalmente
por generar enfermedades de tipo respiratorio y gastrointestinale
Expression of MicroRNAs in Periodontal Disease: A Systematic Review
No full textIntroduction. Periodontal disease (PD) is a chronic inflammation of the soft tissues that support the structure of the tooth, and
miRNAs are highly dynamic molecules that participate in the regulation of gene expression interfering with multiple genetic
targets. The dysregulation of the expression of miRNAs has been associated with different types of pathologies; therefore, they
are excellent molecules to be studied as biomarkers. Material and Methods. A search was made in the electronic databases of
PubMed, Scopus, and Science Direct. The following key words were used: “microRNAs,” “miRNAs,” “periodontal disease,”
“periodontitis,” and “biomarker”; employee independent search strategies with the Boolean operators “OR” and “AND”; a
further search of the references of the selected studies was performed to detect potential studies that met the selection criteria.
The data recollected from each article were author, country, year of publication, sample size, type of sample used to identify
miRNAs, methodology used to identify miRNAs, type of periodontal disease, and miRNAs identified. Results. Of the 13 selected
studies, 6 used gingival tissue as a sample for the identification of miRNAs, 3 used gingival fluid, 2 used saliva, 1 used serum,
and another used periodontal tissue. Chronic periodontitis was the most studied periodontal disease in 9 of the 13 selected
articles; 7 used microarrays as the main technique for the identification of miRNAs. qRT-PCR was the assay choice to validate
the identified miRNAs. Conclusion. The main type of periodontal disease on which most studies are focused is chronic
periodontitis, with the main miRNAs being hsa-miR-146a, hsa-miR-146b, hsa-miR-155, and hsa-miR-200. This systematic
review is one of the first to carry out an analysis of the current role of miRNAs in PD as biomarkers
Dynamic Regulation of Peroxisomes and Mitochondria during Fungal Development
No full textPeroxisomes and mitochondria are organelles that perform major functions in the cell and whose activity is very closely associated. In fungi, the function of these organelles is critical for many developmental processes. Recent studies have disclosed that, additionally, fungal development comprises a dynamic regulation of the activity of these organelles, which involves a developmental regulation of organelle assembly, as well as a dynamic modulation of the abundance, distribution, and morphology of these organelles. Furthermore, for many of these processes, the dynamics of peroxisomes and mitochondria are governed by common factors. Notably, intense research has revealed that the process that drives the division of mitochondria and peroxisomes contributes to several developmental processes—including the formation of asexual spores, the differentiation of infective structures by pathogenic fungi, and sexual development—and that these processes rely on selective removal of these organelles via autophagy. Furthermore, evidence has been obtained suggesting a coordinated regulation of organelle assembly and dynamics during development and supporting the existence of regulatory systems controlling fungal development in response to mitochondrial activity. Gathered information underscores an important role for mitochondrial and peroxisome dynamics in fungal development and suggests that this process involves the concerted activity of these organelles
Video_2_Distinct Contributions of the Peroxisome-Mitochondria Fission Machinery During Sexual Development of the Fungus Podospora anserina.MOV
No full textMitochondria and peroxisomes are organelles whose activity is intimately associated and that play fundamental roles in development. In the model fungus Podospora anserina, peroxisomes and mitochondria are required for different stages of sexual development, and evidence indicates that their activity in this process is interrelated. Additionally, sexual development involves precise regulation of peroxisome assembly and dynamics. Peroxisomes and mitochondria share the proteins mediating their division. The dynamin-related protein Dnm1 (Drp1) along with its membrane receptors, like Fis1, drives this process. Here we demonstrate that peroxisome and mitochondrial fission in P. anserina depends on FIS1 and DNM1. We show that FIS1 and DNM1 elimination affects the dynamics of both organelles throughout sexual development in a developmental stage-dependent manner. Moreover, we discovered that the segregation of peroxisomes, but not mitochondria, is affected upon elimination of FIS1 or DNM1 during the division of somatic hyphae and at two central stages of sexual development—the differentiation of meiocytes (asci) and of meiotic-derived spores (ascospores). Furthermore, we found that FIS1 and DNM1 elimination results in delayed karyogamy and defective ascospore differentiation. Our findings reveal that sexual development relies on complex remodeling of peroxisomes and mitochondria, which is driven by their common fission machinery.</p
Video_5_Distinct Contributions of the Peroxisome-Mitochondria Fission Machinery During Sexual Development of the Fungus Podospora anserina.MOV
No full textMitochondria and peroxisomes are organelles whose activity is intimately associated and that play fundamental roles in development. In the model fungus Podospora anserina, peroxisomes and mitochondria are required for different stages of sexual development, and evidence indicates that their activity in this process is interrelated. Additionally, sexual development involves precise regulation of peroxisome assembly and dynamics. Peroxisomes and mitochondria share the proteins mediating their division. The dynamin-related protein Dnm1 (Drp1) along with its membrane receptors, like Fis1, drives this process. Here we demonstrate that peroxisome and mitochondrial fission in P. anserina depends on FIS1 and DNM1. We show that FIS1 and DNM1 elimination affects the dynamics of both organelles throughout sexual development in a developmental stage-dependent manner. Moreover, we discovered that the segregation of peroxisomes, but not mitochondria, is affected upon elimination of FIS1 or DNM1 during the division of somatic hyphae and at two central stages of sexual development—the differentiation of meiocytes (asci) and of meiotic-derived spores (ascospores). Furthermore, we found that FIS1 and DNM1 elimination results in delayed karyogamy and defective ascospore differentiation. Our findings reveal that sexual development relies on complex remodeling of peroxisomes and mitochondria, which is driven by their common fission machinery.</p
Data_Sheet_3_Distinct Contributions of the Peroxisome-Mitochondria Fission Machinery During Sexual Development of the Fungus Podospora anserina.PDF
No full textMitochondria and peroxisomes are organelles whose activity is intimately associated and that play fundamental roles in development. In the model fungus Podospora anserina, peroxisomes and mitochondria are required for different stages of sexual development, and evidence indicates that their activity in this process is interrelated. Additionally, sexual development involves precise regulation of peroxisome assembly and dynamics. Peroxisomes and mitochondria share the proteins mediating their division. The dynamin-related protein Dnm1 (Drp1) along with its membrane receptors, like Fis1, drives this process. Here we demonstrate that peroxisome and mitochondrial fission in P. anserina depends on FIS1 and DNM1. We show that FIS1 and DNM1 elimination affects the dynamics of both organelles throughout sexual development in a developmental stage-dependent manner. Moreover, we discovered that the segregation of peroxisomes, but not mitochondria, is affected upon elimination of FIS1 or DNM1 during the division of somatic hyphae and at two central stages of sexual development—the differentiation of meiocytes (asci) and of meiotic-derived spores (ascospores). Furthermore, we found that FIS1 and DNM1 elimination results in delayed karyogamy and defective ascospore differentiation. Our findings reveal that sexual development relies on complex remodeling of peroxisomes and mitochondria, which is driven by their common fission machinery.</p
Video_6_Distinct Contributions of the Peroxisome-Mitochondria Fission Machinery During Sexual Development of the Fungus Podospora anserina.MOV
No full textMitochondria and peroxisomes are organelles whose activity is intimately associated and that play fundamental roles in development. In the model fungus Podospora anserina, peroxisomes and mitochondria are required for different stages of sexual development, and evidence indicates that their activity in this process is interrelated. Additionally, sexual development involves precise regulation of peroxisome assembly and dynamics. Peroxisomes and mitochondria share the proteins mediating their division. The dynamin-related protein Dnm1 (Drp1) along with its membrane receptors, like Fis1, drives this process. Here we demonstrate that peroxisome and mitochondrial fission in P. anserina depends on FIS1 and DNM1. We show that FIS1 and DNM1 elimination affects the dynamics of both organelles throughout sexual development in a developmental stage-dependent manner. Moreover, we discovered that the segregation of peroxisomes, but not mitochondria, is affected upon elimination of FIS1 or DNM1 during the division of somatic hyphae and at two central stages of sexual development—the differentiation of meiocytes (asci) and of meiotic-derived spores (ascospores). Furthermore, we found that FIS1 and DNM1 elimination results in delayed karyogamy and defective ascospore differentiation. Our findings reveal that sexual development relies on complex remodeling of peroxisomes and mitochondria, which is driven by their common fission machinery.</p
