83 research outputs found
Glial cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence
No full textTese de doutoramento, Ciências Biomédicas (Imunologia), Universidade de Lisboa, Faculdade de Medicina, 2017In this thesis we demonstrate that three distinct players form a novel multi-tissue defence unit in the intestinal wall: group 3 of innate lymphoid cells (ILC3s), intestinal epithelial cells and enteric glial cells (EGCs). This interplay reveals a neuro-immune interaction unit that regulates epithelial homeostasis and mucosal defense. ILC3s are major regulators at mucosal surfaces being critical in tissue repair and in the maintenance of gut homeostasis. Intestinal ILC3 – that mainly aggregate into cryptopatches - integrate environmental signals leading to the production of the proinflammatory cytokines IL-22 and IL-17. IL-22 in turn induces intestinal epithelial cells to produce antimicrobial peptides and mucus. We found that ILC3s express high levels of RET, a neuroregulatory receptor for GDNF family ligands (GFLs). In order to address the effect of RET in ILC3s development and function RET-deficient mice foetal liver chimeras were analyzed. Interestingly a decrease of IL-22 expressing ILC3s was observed when compared to WT controls. In addition a RET gain of function model (RetMEN2B) resulted in increased IL-22 expressing ILC3s. In line with these experiments, cell-autonomous ablation of RET in Rorγt expressing cells was performed. RorγtCreRetfl/fl, (RetΔ) mice had decrease IL-22 expressing ILC3s and a reduction of epithelial reactivity genes such as mucins and defensins comparing with their littermate controls. Upon infection with the attaching and effacing bacteria Citrobacter rodentium, RetΔ mice had marked gut inflammation, reduced IL-22 producing ILC3, increased C. rodentium infection and translocation, reduced epithelial reactivity genes, increased weight loss and reduced survival. All these data together, suggest that RET cell autonomous ILC3 signals regulate IL-22 production. Signals downstream of Ret were regulated via GFLs which directly controled rapid phosphorylation of the p38 MAPK/ERK-AKT cascade and STAT3 activation in ILC3s. In turn, STAT3 bound to the Il22 promoter to induce transcription. Finally, we found that enteric glial cells integrated commensal and environmental signals to produce GFLs that control IL-22 production. Physical localization of glial cells in the vicinity of ILC3 was observed taking advantage of double reporter mice for GFAP (glial fibrillary acidic protein) and RET. Enteric glial cells had a stellate shape morphology, projecting into cryptopatches. In vitro co-culture studies showed EGCs capacity to produce GFLs in response to TLR2 and TLR4 activation and IL-1β and IL-33 stimulation, promoting IL-22 production by ILC3s. In vivo studies with DSS induced colitis in glial specific Myd88 deficient mice (Gfap-Cre.Myd88Δ) showed an increase of gut inflammation and weight loss along with a reduced expression of intestinal GFLs and ILC3-derived IL-22 levels compared with their littermate controls. When infected with Citrobacter Rodentium Myd88 deficient mice exhibited a pronounced susceptibility to bowel inflammation and infection. In summary, we were able to show that the enteric glial cells sense environmental cues through MYD88 to produce GFLs that in turn activate RET expressing ILC3s and via MAP kinase and STAT3 induce the transcription of Il22. The production of IL-22 promotes the expression of defence and repair genes. Thus, this novel glial-ILC3 epithelial unit is critical in the maintenance of intestinal homeostasis providing protection and repairing the epithelial barrier after injury
Glial cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence
No full textTese de doutoramento, Ciências Biomédicas (Imunologia), Universidade de Lisboa, Faculdade de Medicina, 2017In this thesis we demonstrate that three distinct players form a novel multi-tissue defence unit in the intestinal wall: group 3 of innate lymphoid cells (ILC3s), intestinal epithelial cells and enteric glial cells (EGCs). This interplay reveals a neuro-immune interaction unit that regulates epithelial homeostasis and mucosal defense. ILC3s are major regulators at mucosal surfaces being critical in tissue repair and in the maintenance of gut homeostasis. Intestinal ILC3 – that mainly aggregate into cryptopatches - integrate environmental signals leading to the production of the proinflammatory cytokines IL-22 and IL-17. IL-22 in turn induces intestinal epithelial cells to produce antimicrobial peptides and mucus. We found that ILC3s express high levels of RET, a neuroregulatory receptor for GDNF family ligands (GFLs). In order to address the effect of RET in ILC3s development and function RET-deficient mice foetal liver chimeras were analyzed. Interestingly a decrease of IL-22 expressing ILC3s was observed when compared to WT controls. In addition a RET gain of function model (RetMEN2B) resulted in increased IL-22 expressing ILC3s. In line with these experiments, cell-autonomous ablation of RET in Rorγt expressing cells was performed. RorγtCreRetfl/fl, (RetΔ) mice had decrease IL-22 expressing ILC3s and a reduction of epithelial reactivity genes such as mucins and defensins comparing with their littermate controls. Upon infection with the attaching and effacing bacteria Citrobacter rodentium, RetΔ mice had marked gut inflammation, reduced IL-22 producing ILC3, increased C. rodentium infection and translocation, reduced epithelial reactivity genes, increased weight loss and reduced survival. All these data together, suggest that RET cell autonomous ILC3 signals regulate IL-22 production. Signals downstream of Ret were regulated via GFLs which directly controled rapid phosphorylation of the p38 MAPK/ERK-AKT cascade and STAT3 activation in ILC3s. In turn, STAT3 bound to the Il22 promoter to induce transcription. Finally, we found that enteric glial cells integrated commensal and environmental signals to produce GFLs that control IL-22 production. Physical localization of glial cells in the vicinity of ILC3 was observed taking advantage of double reporter mice for GFAP (glial fibrillary acidic protein) and RET. Enteric glial cells had a stellate shape morphology, projecting into cryptopatches. In vitro co-culture studies showed EGCs capacity to produce GFLs in response to TLR2 and TLR4 activation and IL-1β and IL-33 stimulation, promoting IL-22 production by ILC3s. In vivo studies with DSS induced colitis in glial specific Myd88 deficient mice (Gfap-Cre.Myd88Δ) showed an increase of gut inflammation and weight loss along with a reduced expression of intestinal GFLs and ILC3-derived IL-22 levels compared with their littermate controls. When infected with Citrobacter Rodentium Myd88 deficient mice exhibited a pronounced susceptibility to bowel inflammation and infection. In summary, we were able to show that the enteric glial cells sense environmental cues through MYD88 to produce GFLs that in turn activate RET expressing ILC3s and via MAP kinase and STAT3 induce the transcription of Il22. The production of IL-22 promotes the expression of defence and repair genes. Thus, this novel glial-ILC3 epithelial unit is critical in the maintenance of intestinal homeostasis providing protection and repairing the epithelial barrier after injury
Glial cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence
No full textTese de doutoramento, Ciências Biomédicas (Imunologia), Universidade de Lisboa, Faculdade de Medicina, 2017In this thesis we demonstrate that three distinct players form a novel multi-tissue defence unit in the intestinal wall: group 3 of innate lymphoid cells (ILC3s), intestinal epithelial cells and enteric glial cells (EGCs). This interplay reveals a neuro-immune interaction unit that regulates epithelial homeostasis and mucosal defense. ILC3s are major regulators at mucosal surfaces being critical in tissue repair and in the maintenance of gut homeostasis. Intestinal ILC3 – that mainly aggregate into cryptopatches - integrate environmental signals leading to the production of the proinflammatory cytokines IL-22 and IL-17. IL-22 in turn induces intestinal epithelial cells to produce antimicrobial peptides and mucus. We found that ILC3s express high levels of RET, a neuroregulatory receptor for GDNF family ligands (GFLs). In order to address the effect of RET in ILC3s development and function RET-deficient mice foetal liver chimeras were analyzed. Interestingly a decrease of IL-22 expressing ILC3s was observed when compared to WT controls. In addition a RET gain of function model (RetMEN2B) resulted in increased IL-22 expressing ILC3s. In line with these experiments, cell-autonomous ablation of RET in Rorγt expressing cells was performed. RorγtCreRetfl/fl, (RetΔ) mice had decrease IL-22 expressing ILC3s and a reduction of epithelial reactivity genes such as mucins and defensins comparing with their littermate controls. Upon infection with the attaching and effacing bacteria Citrobacter rodentium, RetΔ mice had marked gut inflammation, reduced IL-22 producing ILC3, increased C. rodentium infection and translocation, reduced epithelial reactivity genes, increased weight loss and reduced survival. All these data together, suggest that RET cell autonomous ILC3 signals regulate IL-22 production. Signals downstream of Ret were regulated via GFLs which directly controled rapid phosphorylation of the p38 MAPK/ERK-AKT cascade and STAT3 activation in ILC3s. In turn, STAT3 bound to the Il22 promoter to induce transcription. Finally, we found that enteric glial cells integrated commensal and environmental signals to produce GFLs that control IL-22 production. Physical localization of glial cells in the vicinity of ILC3 was observed taking advantage of double reporter mice for GFAP (glial fibrillary acidic protein) and RET. Enteric glial cells had a stellate shape morphology, projecting into cryptopatches. In vitro co-culture studies showed EGCs capacity to produce GFLs in response to TLR2 and TLR4 activation and IL-1β and IL-33 stimulation, promoting IL-22 production by ILC3s. In vivo studies with DSS induced colitis in glial specific Myd88 deficient mice (Gfap-Cre.Myd88Δ) showed an increase of gut inflammation and weight loss along with a reduced expression of intestinal GFLs and ILC3-derived IL-22 levels compared with their littermate controls. When infected with Citrobacter Rodentium Myd88 deficient mice exhibited a pronounced susceptibility to bowel inflammation and infection. In summary, we were able to show that the enteric glial cells sense environmental cues through MYD88 to produce GFLs that in turn activate RET expressing ILC3s and via MAP kinase and STAT3 induce the transcription of Il22. The production of IL-22 promotes the expression of defence and repair genes. Thus, this novel glial-ILC3 epithelial unit is critical in the maintenance of intestinal homeostasis providing protection and repairing the epithelial barrier after injury
The prognostic power of [11C]methionine PET in IDH-wildtype diffuse gliomas with lower-grade histological features: venturing beyond WHO classification
No full textPurpose IDH-wildtype (IDH-wt) diffuse gliomas with histological features of lower-grade gliomas (LGGs) are rare and heterogeneous primary brain tumours. [11C]Methionine (MET) positron emission tomography (PET) is commonly used to evaluate glial neoplasms at diagnosis. The present study aimed to assess the prognostic value of MET PET in newly diagnosed, treatment naïve IDH-wt gliomas with histological features of LGGs. Methods Patients with a histological diagnosis of IDH-wt LGG who underwent preoperative (< 100 days) MET PET/CT and surgery were retrospectively included. Qualitative and semi-quantitative analyses of MET PET images were performed. Progression-free survival (PFS) and overall survival (OS) were analysed by Kaplan–Meier curves. Cox proportional-hazards regression was used to test the association of imaging and clinical data to PFS and OS. Results We included 48 patients (M:F = 25:23; median age 55). 39 lesions were positive and 9 negative at MET PET. Positive MET PET was significantly associated with shorter median PFS (15.7 months vs. not reached, p = 0.0146) and OS time (32.6 months vs. not reached, p = 0.0253). Incomplete surgical resection and higher TBRmean values were independent predictors of shorter PFS on multivariate analysis (p < 0.001 for both). Higher tumour grade and incomplete surgical resection were independent predictors of OS at multivariate analysis (p = 0.027 and p = 0.01, respectively). Conclusion MET PET is useful for the prognostic stratification of patients with IDH-wt glial neoplasms with histological LGGs features. Considering their huge biological heterogeneity, the combination of MET PET and molecular analyses may help to improve the prognostic accuracy in these diffuse gliomas subset and influence therapeutic choices accordingly
[pt] SOLIDARIEDADE EM CRISE: UMA RECONSTRUÇÃO NORMATIVA À LUZ DA ALTERIDADE E DO PARADIGMA DO REFUGIADO
No full textRaman Spectroscopy and Machine Learning for IDH Genotyping of Unprocessed Glioma Biopsies
Get PDFIsocitrate dehydrogenase (IDH) mutational status is pivotal in the management of gliomas. Patients with IDH-mutated (IDH-MUT) tumors have a better prognosis and benefit more from extended surgical resection than IDH wild-type (IDH-WT). Raman spectroscopy (RS) is a minimally invasive optical technique with great potential for intraoperative diagnosis. We evaluated the RS’s ability to characterize the IDH mutational status onto unprocessed glioma biopsies. We extracted 2073 Raman spectra from thirty-eight unprocessed samples. The classification performance was assessed using the eXtreme Gradient Boosted trees (XGB) and Support Vector Machine with Ra-dial Basis Function kernel (RBF-SVM). Measured Raman spectra displayed differences between IDH-MUT and IDH-WT tumor tissue. From the 103 Raman shifts screened as input features, the cross-validation loop identified 52 shifts with the highest performance in the distinction of the two groups. Raman analysis showed differences in spectral features of lipids, collagen, DNA and choles-terol/phospholipids. We were able to distinguish between IDH-MUT and IDH-WT tumors with an accuracy and precision of 87%. RS is a valuable and accurate tool for characterizing the mutational status of IDH mutation in unprocessed glioma samples. This study improves RS knowledge for future personalized surgical strategy or in situ target therapies for glioma tumors
The Predictive Role of Radiomics in Breast Cancer Patients Imaged by [18F]FDG PET: Preliminary Results from a Prospective Cohort
No full textBackground: Recently, radiomics has emerged as a possible image-derived biomarker, predominantly stemming from retrospective analyses. We aimed to prospectively assess the predictive role of [18F]FDG-PET radiomics in breast cancer (BC). Methods: Patients affected by stage I–III BC eligible for neoadjuvant chemotherapy (NAC) staged with [18F]FDG-PET/CT were prospectively enrolled. The pathological response to NAC was assessed on surgical specimens. From each primary breast lesion, we extracted radiomic PET features and their predictive role with respect to pCR was assessed. Uni- and multivariate statistics were used for inference; principal component analysis (PCA) was used for dimensionality reduction. Results: We analysed 93 patients (53 HER2+ and 40 triple-negative (TNBC)). pCR was achieved in 44/93 cases (24/53 HER2+ and 20/40 TNBC). Age, molecular subtype, Ki67 percent, and stage could not predict pCR in multivariate analysis. In univariate analysis, 10 radiomic indices resulted in p < 0.1. We found that 3/22 radiomic principal components were discriminative for pCR. Using a cross-validation approach, radiomic principal components failed to discriminate pCR groups but predicted the stage (mean accuracy = 0.79 ± 0.08). Conclusions: This study shows the potential of PET radiomics for staging purposes in BC; the possible role of radiomics in predicting the pCR response to NAC in BC needs to be further investigated
University seniors and recognition: access, permanence and challenges at the Federal University of Viçosa
No full textA inclusão, a permanência e os desafios enfrentados pelos idosos universitários, estudantes da Universidade Federal de Viçosa (UFV), são os temas deste trabalho. A pesquisa foi desenvolvida na respectiva universidade e foram entrevistados sete estudantes com idade igual ou acima de sessenta anos que estavam regularmente matriculados na instituição. O objetivo geral foi compreender as relações de reconhecimento que os alunos idosos mantêm com a Universidade. A metodologia utilizada para a melhor compreensão do corpus investigado foi a Análise de Discurso (AD) de linha francesa. Os dados analisados foram coletados por meio de entrevistas semiestruturadas. Na análise do corpus, identificamos o funcionamento de 10 (dez) temas dominantes, que se materializaram nos textos dos sujeitos idosos. Estes interdiscursos foram agrupados de acordo com as três esferas da Teoria do Reconhecimento de Axel Honneth: Amor, Direito e Solidariedade. Dentre os dez temas, construídos através do processo de-superficialização do corpus, foram definidos para análise as relações intergeracionais, os estereótipos sobre a velhice e idosos com acessibilidade e deficiência na UFV. Após a análise dos discursos dos sujeitos entrevistados, identificamos uma heterogeneidade nos modos como esses sujeitos atribuem sentidos sobre sua relação com a Universidade. No primeiro tema - relações intergeracionais - os universitários falaram sobre as vivências e o aprendizado com os colegas mais novos, o sentimento de exclusão dos grupos de trabalho e a ausência do reconhecimento do próprio sujeito enquanto idoso. Nesta esfera, foram manifestados exemplos de desrespeitos sofridos, apesar da construção simultânea da autoconfiança necessária para participar da vida pública. Observamos, pois, que suas atitudes têm relação com histórias de êxito na construção do vínculo amoroso com os familiares. No que tange à esfera do Direito - estereótipos sobre a velhice -, notamos diferença no tratamento destinado às idosas e aos idosos. As idosas frequentemente são questionadas sobre o motivo do retorno aos estudos, além de acusações sobre roubar o lugar do jovem. Para os idosos não há repreensões. Deduzimos, portanto, que eles possuem reconhecimento nesta esfera, ao contrário das mulheres idosas que, ao relatarem seus desconfortos, demonstraram compreender que seus direitos são diferentes dos oferecidos aos outros universitários. E na Solidariedade - idosos com acessibilidade e deficiência na UFV - percebemos que as sugestões de melhorias são tão diversas quanto os modos de viver a velhice por cada um dos entrevistados. Consideramos, igualmente, que para alguns sujeitos as lutas por reconhecimento na Universidade não são travadas por todos os entrevistados. Diante disso, propomos encaminhamentos para o fortalecimento das políticas para idosos na universidade, que abrangem a educação sobre a velhice, o processo de envelhecimento e os direitos dos idosos, o estímulo à convivência entre pessoas de diferentes idades e a ampliação dos atendimentos da Unidade de Políticas Inclusivas (UPI). Palavras-chave: Educação Superior. Idosos. Reconhecimento. Discurso.Inclusion, permanence and challenges faced by elderly university students, students at the Federal University of Viçosa (UFV), are the themes of this work. The research was carried out at the respective university and seven students were interviewed, aged 60 years or over, who were regularly enrolled at the institution. The general objective was to understand the recognition relationships that elderly students have with the University. The methodology used for a better understanding of the investigated corpus was Discourse Analysis (AD) of French line. The analyzed data were collected through semi-structured interviews. In the analysis of the corpus, we identified the functioning of 10 (ten) dominant themes, which materialized in the texts of the elderly subjects. These interdiscourses were grouped according to the three spheres of Axel Honneth's Theory of Recognition: Love, Law and Solidarity. Among the ten themes, constructed through the corpus de-superficialization process, intergenerational relationships, stereotypes about old age and elderly with accessibility and disability at UFV were defined for analysis. After analyzing the speeches of the interviewed subjects, we identified a heterogeneity in the ways in which these subjects attribute meanings about their relationship with the University. In the first theme - intergenerational relations - the university students talked about their experiences and learning with their younger colleagues, the feeling of exclusion from work groups and the lack of recognition of the subject as an elderly person. In this sphere, examples of disrespect suffered were expressed, despite the simultaneous construction of the self-confidence necessary to participate in public life. We observed, therefore, that their attitudes are related to success stories in building a loving bond with family members. Regarding the sphere of Law - stereotypes about old age - we note a difference in the treatment for the elderly and the elderly. Elderly women are often asked about the reason for returning to school, as well as accusations about stealing the young man's place. There is no reprimand for the elderly. We deduce, therefore, that they have recognition in this sphere, unlike elderly women who, when reporting their discomforts, demonstrated to understand that their rights are different from those offered to other university students. And in Solidarity - elderly people with accessibility and disabilities at UFV - we noticed that the suggestions for improvements are as diverse as the ways of living old age for each of the interviewees. We also consider that for some subjects the struggles for recognition at the University are not fought by all respondents. In view of this, we propose guidelines for strengthening policies for the elderly at the university, which include education about old age, the aging process and the rights of the elderly, encouraging coexistence between people of different ages and expanding care at the Health Care Unit. Inclusive Policies (UPI). Keywords: College education. Seniors. Recognition. Speech.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES
Mixtures of bentonite with tropical soil and mining tailings for sanitary landfill liners
Get PDFO intuito da presente pesquisa foi avaliar o potencial de aplicação de misturas de bentonita com um solo tropical e com um rejeito de mineração, para uso como barreiras impermeabilizantes de aterros sanitários. Para tal, desenvolveu-se um programa experimental de forma a analisar o comportamento mecânico e hidráulico dos materiais em seu estado puro (sem aditivo) e em misturas com 2%, 4%, 6% e 8% de bentonita, em termos de massa seca do material. Nos materiais no estado puro e suas misturas foram realizados ensaios de compactação na energia Proctor Normal, de condutividade hidráulica em permeâmetro de parede flexível, triaxiais adensados e não drenados (CIU) e de adensamento com deformação controlada (CRS). As curvas de compactação mostraram que, tanto para as misturas de solo tropical-bentonita como de rejeito-bentonita, houve um aumento no teor de umidade ótimo e uma redução no peso específico seco máximo, conforme o aumento no teor de bentonita. Os valores de condutividade hidráulica indicaram uma diminuição com o acréscimo de bentonita, tanto para o solo quanto para o rejeito. Observou-se que, com o aumento da tensão de adensamento dos corpos de prova, houve redução na condutividade hidráulica, tanto para as misturas solo-bentonita como rejeito- bentonita. Com relação ao solo, o teor de 8% de bentonita reduziu sua permeabilidade para valores aceitáveis para revestimento de fundo de aterros sanitários. Já para as misturas de rejeito-bentonita, os resultados indicaram uma redução da permeabilidade com a adição de bentonita. Contudo, mesmo o maior teor de bentonita utilizado (8%) não resultou em redução da permeabilidade para os valores recomendados. Em relação à resistência ao cisalhamento, constatou-se que, tanto para as misturas solo-bentonita como rejeito-bentonita, houve, no geral, uma redução desta com o aumento no teor de bentonita. A compressibilidade do solo tropical aumentou com o acréscimo de bentonita. Já para o rejeito, somente foram constatados aumentos na compressibilidade para as misturas com 4% e 6% de bentonita, sendo observada uma ligeira redução na mistura com 8% de bentonita. Palavras-chave: Solo-bentonita. Rejeito-bentonita. Impermeabilização. Aterros sanitários.The intent of this research was to evaluate the potential of application of bentonite mixtures with a tropical soil and with a mining tailings, for use as sanitary landfill liners. For this, an experimental program was developed in order to analyze the mechanical and hydraulic behavior of the materials in pure state (no additive) and in mixtures of 2%, 4%, 6% and 8% of bentonite, in terms of the dry mass of the material. In the pure state materials and their mixtures, compaction tests were performed using Proctor Normal energy, hydraulic conductivity in a flexible wall permeameter, consolidated undrained triaxial tests (CIU) and constant rate-of- strain tests (CRS). The compaction curves showed that, for both tropical soil-bentonite and tailings-bentonite mixtures, there was an increase in the optimum moisture content and a reduction in the maximum dry specific weight, according to the increase in bentonite content. The hydraulic conductivity values indicated a decrease with the addition of bentonite, both for the soil and for the tailings. With the increase in the values of consolidation stresses, it was observed that there was a reduction in hydraulic conductivity for both soil-bentonite and tailings-bentonite mixtures. Regarding the soil, the 8% content of bentonite reduced its permeability to acceptable values for sanitary landfill liner. For the tailings-bentonite mixtures, the results indicated a reduction in permeability with the addition of bentonite. However, these results indicated that even the highest content of bentonite used (8%) did not reduce the permeability to recommended values. Regarding shear strength, it was found that, for both soil- bentonite and tailings-bentonite mixtures, there was, in general, a reduction as the bentonite content increased. The compressibility of the tropical soil increased with the addition of bentonite. As for the tailings, there were only increases in compressibility for the mixtures with 4% and 6% of bentonite, with a slight reduction being observed in the mixture with 8% of bentonite. Keywords: Soil-bentonite. Tailings-bentonite. Impermeabilization. Sanitary landfills
Perspectives on corporate volunteering programs: why they matter and new directions
No full textResearch on the volunteering experiences of employees and their relationships with personal and organizational outcomes has blossomed in recent years. However, much of this research has not distinguished between employees’ engagement in personal and corporate-sponsored volunteering programs. Personal volunteering (i.e., employees volunteering their own time to causes that support the community) is distinct from corporate volunteering – employees’ participation in corporate-sponsored activities, with support from their employer in terms of paid time or other supporting resources. Given our shortage of knowledge regarding the impacts of corporate-sponsored volunteering programs in particular, this symposium brings together five presentations that seek to advance our understanding of the impacts of corporate volunteering programs from a variety of perspectives. Collectively, the papers use a combination of quantitative and qualitative methods to provide novel insights into why corporate volunteering matters, the processes through which organizations design and implement these programs, the impacts such programs can have on employees, the expectations various stakeholders in these programs hold of each other, and how these programs can be designed to be sustainable over time. The symposium will close with an audience discussion led by our discussant on the implications of the five papers for the study of corporate volunteering programs. Employee volunteering as a change catalyst Author: Katerina Gonzalez; Suffolk University Author: Florencio F. Portocarrero; London School of Economics and Political Science Broadening the Social Impact: How Volunteering Enables Servant Leadership Author: Haoying Xu; Stevens Institute of Technology Author: John Lynch; University of Illinois at Chicago Author: Sandy J. Wayne; Author: Siyi Tao; Employee volunteering programs: a marginal and essentially performative CSR tool Author: Bethania Antunes; London School of Economics and Political Science Author: Cecile Guillaume; University of Surrey Author: Lisa Jean Cafora; University of Surrey The (Mis)Alignment of Expectations across Corporate Volunteering Program Stakeholders Author: Jonathan Edward Booth; Author: John Lynch; University of Illinois at Chicago Author: Aaron Aujla; London School of Economics and Political Science Author: Haoying Xu; Stevens Institute of Technology When in Rome? Selling centralized HR policies across subsidiaries: A qualitative investigation Author: Kiera Dempsey-Brench; Trinity College Dublin, The University of Dublin Author: Mihwa Seong; King's College London Author: Amanda Shantz; Not Associate
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