1,721,276 research outputs found
Entrevista sobre a gestão de pessoas em áreas de licitações com o diretor da Central de Compras da Câmara dos Deputados e professor da Enap, Renato Fenili
Get PDFNa vigésima entrevista exclusiva para a Comunidade de Prática de Compras Públicas da Escola Nacional de Administração Pública (Enap), o diretor da Central de Compras da Câmara dos Deputados e professor da Enap, Renato Fenili, falou sobre a gestão de pessoas em áreas de licitações. As perguntas foram elaboradas pela professora da Enap e servidora da Advocacia-Geral da União, Grasielle de Oliveira Abrantes.4 páginasLogística e Compras Pública
NOVEL BIOACTIVE AND BIODEGRADABLE BIOMATERIALS OF POLY(AMIDOMINE) STRUCTURE
Get PDFPhD thesis of Fabio Fenili R07830
Tutor: Prof. Elisabetta Ranucci
Coordinator: Prof. Dominique Roberto
NOVEL BIOACTIVE AND BIODEGRADABLE BIOMATERIALS OF POLY(AMIDOAMINE)S STRUCTURE
Biomaterials are any materials designed to interface with biological system to evaluate, support or replace any tissue, organ or body function. Metals, ceramics, polymers and composites are biomaterials commonly used every day for biomedical applications in the form of sutures, vascular grafts, heart valves, intraocular lenses, dental and orthopaedic implants, pacemakers, biosensors, drug delivery systems, gene carriers, etc.
Compared to the other types of biomaterials, polymeric biomaterials offer the advantages that can be prepared in different compositions with a variety of structures and properties. In particular, in the case of biodegradable polymers, their physical, chemical, and biological characteristics as well as degradation rate can be tuned to meet specific applications.
Among polymeric biomaterials, poly(amidoamine)s (PAA), a family of biodegradable and biocompatible polymers, can be considered as promising materials for different pharmaceuticals and biotechnological applications.
The present PhD project aimed at the design of novel poly(amidoamine)s biomaterials of different architecture for diversified biomedical applications, each representing a distinct research sub-objective.
Poly(amidoamine)-gelonin conjugates as potential anti-cancer drugs.
Two PAAs bearing pendant 2-ethenyldithiopyridine (ISA1-SSPy and ISA23-SSPy) were used to investigate their ability to mediate intracellular delivery of the type I ribosome-inactivating gelonin. The strategy adopted consists of a three step synthesis. In the first step, a cystamine crosslinked PAA was synthesised generating a hydrophilic tri-dimensional network. In the second step, the hydrogel obtained was de-reticulated by direct disulfide exchange reaction with dipyridyl disulfide. Finally, the linear and soluble PAA-SSPy thus obtained was conjugated via thiol-disulfide exchange reaction to a thiol-containing gelonin, a ribosom inactivating protein. Two different recombinant protein, encoding gelonin with an N-terminal 6xHistidine (H or His) and V5 epitope tag (6H-V5 Gelonin) and gelonin encorporating a C-terminal HA and 6xHis tag flanking a cysteine residue (Gelonin HA-Cys-6H) were prepared introducing the required tag on a gelonin plasmid by polymerase chain reaction (PCR), isolated and purified from E. Coli.
In vitro experiments were performed on B16F10 cells using non-toxic concentration of gelonin and polymeric samples up to 2 mg/ml. ISA1-SSPy promoted the intracytoplasmic delivery of gelonin more efficiently than the parent non-functionalized ISA1 with IC50 values of 100 μg/ml.
The results obtained for ISA1-SSPy-HA-Cys-6H, designed to have a covalent bound between the polymeric vector and the toxin, and ISA1-SSPy-6H-V5 Gelonin were the same, suggesting a non specific conjugation to the thiol-groups in the Gelonin HA-Cys-6H.
This findings could be attributed both to the ability of ISA1-SSPy to react with protein’s disulfide groups and to the interactions between the ethenyl-dithiopyridine pendants and the hydrophobic domains of the protein, giving stable complexes. ISA23-SSPy was unable to mediate toxin delivery.
Tricarbonyl-rhenium complexes of a thiol-functionalized amphoteric poly(amidoamine).
In this work we investigate the possibility to design new polymeric radiopharmaceuticals using poly(amidoamine)s polymers (PAA)s and [Re(CO)3(H2O)3]+. An amphoteric thiol-functionalized ISA23 copolymer (ISA23SH10%) has been obtained following a two step pathway: the first was the synthesis of polymers containing cystamine-m-Boc pendants, the second one was the reduction of the disulphide bond in the presence of dithiotreitol (DTT) as reducing agent. The copolymer was able to tightly bind up to 0.8 equivalents of Re(CO)3+ fragments, with respect to the thiol groups. The polymeric complexes, containing 0.5 or 0.8 equivalents of rhenium, respectively, were easily obtained by reacting ISA23SH10% with [Re(CO)3(H2O)3](CF3SO3), in aqueous solution, at pH 5.5. The complexes maintained the water solubility of the parent polymer and were stable in physiological conditions and also in the presence of cysteine. The coordination of the Re(CO)3 fragment involves the cysteamine-deriving moiety.
A detailed 1H, 13C and 15N NMR characterization of ISA23SH10% and of complex with 0.8 equivalents of Re(CO)3+ provided a clear evidence that the binding of rhenium occurs by chelation through the S and N atoms of the -amino-thiolate fragment. A morphological evaluation by TEM analysis showed that both complexes form nanoparticles with a regular spherical morphology and a narrow size distribution. In vivo toxicological tests showed that ISA23SH10% is highly biocompatible, with a maximum tolerated dose of 500 mg/kg. Preliminary biological studies in vitro and in vivo have been performed also on both complexes. No hemolytic activity was observed, up to a concentration of 5 mg/mL, neither citotoxicity effect was observed on Hela cell after 48 h of incubation. No toxic side effects were observed after the intravenous injection in mice of the two complexes in doses up to 20 mg/kg.
Poly(amidoamine)-based hydrogels as scaffold for tissue engineering applications.
In this work a new synthetic method has been developed leading to PAA hydrogels with improved mechanical strength and good biological properties for tissue engineering applications. In particular a two-step pathway has been followed: in the first step an acryloyl end-capped linear Agma1 oligomer was synthesised using a controlled excess of the bisacrylamide; in the second step the oligomer was photopolymerized by UV irradiation producing hydrogels with the required mechanical characteristics.
Using this new synthetic procedure, PAA-UV made hydrogels with different form and shape were prepared. In particular, tubular scaffolds with 1 mm inner diameter were tested in vivo as conduit for nerve regeneration in a rat sciatic nerve cut model. The implants were analyzed at 30, 90 and 180 days post-surgery and resulted particularly promising in many important respects, such as biodegradability, biocompatibility, lack of inflammatory reaction upon degradation and capability of promoting optimum morphological and functional nerve regeneration. The regenerated nerves showed several interesting signs of morphological improvements even at 30 days post-surgery. At 180 days the scaffold was almost completely reabsorbed and the regenerated nerve morphologically comparable to the control animals.
Cationic PAA as non-viral vector for gene therapy.
Recently, a new linear and amphoteric but prevailing cationic PAA, nicknamed Agma1 (Scheme 4) has been successfully tested as trasfection promoters in vitro without any signs of toxicity is despite its polycationic behavior. Based on this premise, in this work, three samples of Agma1 having different molecular weights, named AGMA5 ( =5100), AGMA10 ( =10100) and AGMA20 ( =20500) were tested as nucleic acid carriers in order to establish the relationship between the molecular weight of the samples and their gene transfer ability. All samples proved able to complex DNA forming stable nanoparticles with positively charged surface and dimensions depending on the weight/weight ratio between polymer and DNA, and pH. The molecular weight of the polymeric samples had a significant influence on their efficiency as DNA carrier. AGMA10 and AGMA20 showed good transfection ability compared to AGMA5 which was almost ineffective even at a 1:100 w/w DNA/polymer ratio. The polyplex nanoparticles proved highly biocompatible and were easily internalized in cells escaping from the endosomal vesicles and mostly localizing in perinuclear region. AGMA10 was chosen for animal experiments and proved effective also in vivo. A 1:30 DNA/AGMA10 polyplex after intravenous administration to mice induced remarkable gene expression in the liver but not in other organs, including lungs without detectable toxic side effects
Reintegração ao convívio familiar : um projeto assistencial de enfermagem ao cliente do Instituto de psiquiatria
Get PDFSeminário compras estratégicas compartilhadas
No full textCompras compartilhadas, conceitos. Vantagens das compras centralizadas. O estudo de caso do Instituto Benjamin Constant.Contém 2 apresentações/slides, com 53 páginas cada.Licitação. ComprasCompras compartilhadas sustentáveis: critérios ambientais com ganhos econômicos: palestrante Renato Cader.Compras públicas compartilhadas: análise processual e mercadológica: palestrante Renato Fenili
Seminário de Planejamento e Governança em Compras Públicas
No full textA Escola Nacional de Administração Pública (Enap) realizou o Seminário Planejamento e Governança em Compras Públicas. A atividade, foi ministrada pelo analista legislativo e diretor da Coordenação de Compras da Câmara dos Deputados, Renato Fenili, e contou com a participação de servidores de órgãos executores e de controle na área de compras públicas. Na abertura dos trabalhos, o diretor de Educação Continuada da Enap, Paulo Marques, falou sobre a relevância da logística para a administração pública e reforçou a Comunidade de Prática de Compras Públicas da Escola como um espaço virtual de interação entre servidores que atuam com o tema. “Em breve lançaremos um programa com foco nos coordenadores-gerais de Tecnologia da Informação e nos dirigentes da área-meio, a ser ministrado na modalidade mista, com poucas horas presenciais, atividades a distância e acompanhamento de couching. Faremos essa experimentação e a expectativa é oferecermos uma edição por ano do programa”, ressaltou Marques. Em seguida, o diretor do Departamento de Normas e Sistemas de Logística do Ministério do Planejamento, Desenvolvimento e Gestão (Delog/MPDG), Wesley Lira, comentou sobre o trabalho que seu departamento vem fazendo. “Temos algumas entregas previstas para este ano. Vale destacar o decreto de reorganização do Sistema Integrado de Serviços Gerais (Sisg); a atualização das instruções normativas necessárias à gestão dos processos de gestão e da governança na área de logística pública; um sistema para registro e controle anual de compras públicas; uma nova instrução normativa de serviços; uma instrução normativa de bens; o aperfeiçoamento dos normativos de compras sustentáveis; bem como uma nova trilha de capacitação. Logo teremos o lançamento de uma ferramenta importante na área de pesquisa de preços, que também ficará disponível para os poderes Legislativo e Judiciário”, frisou Lira. Durante sua exposição, Renato Fenili abordou os principais conceitos de planejamento e governança das compras públicas sob a luz da doutrina e da jurisprudência do Tribunal de Contas da União. “Historicamente não tivemos a preocupação com as compras públicas como um subprocesso da logística pública. Por anos nos preocupamos somente em atender a legislação pertinente para não corrermos riscos de questionamento pelos órgãos de controle. Somente de três anos para cá é que começamos a nos preocupar com boas práticas de gestão associadas ao tema”, enfatizou. “No momento de implantação da governança é necessária uma liderança capaz e comprometida, mas, depois, as instituições públicas necessitam de um sistema racional legal ao estilo weberiano, de forma que, uma vez implantada a estrutura de governança corporativa, a alta gestão não poderá prejudicá-la, somente aperfeiçoá-la”, completou Fenili.01 apresentação em slides: 98 p.Administração PúblicaGestão PúblicaLicitação. Compra
Reti istituzionali per un turismo intergenerazionale di qualità: ricerche sperimentazioni sul territorio nazionale
No full textA partire dalle prime ricerche del Laboratorio Incontri Generazionali dell'Università degli Studi di Milano (1998) su "Linguaggi informatici e comunicazione intergenerazionale" e assumendo come primo obiettivo quello di: "socializzare le generazioni, socializzando le istituzioni" (A. Albanese, 1999, 2000) studi di turismo intergenerazionale sono stati avviati nel nord Italia.
Ci concentreremo in particolare sull'efficacia del programma “Nonni e Nipoti”, orientato alla creazione e sperimentazione di incontri specifici e occasioni di contatto (basati su percorsi turistici) tra persone di generazioni diverse (adolescenti vs anziani) nella comunità di accoglienza di Sfruz (Tn) e di altre località.
Si evidenzia inoltre come il turismo intergenerazionale sia una forma di turismo dolce, di turismo sociale, di turismo pedagogico di turismo responsabile e sostenibile. La vacanza concorre pertanto alla promozione del benessere psico-fisico e del benessere sociale, favorisce l’ inclusione sociale, diventa una forma di contrasto delle devianze, si dipana secondo il principio dell’imparare divertendosi, diventa un incontro con l’alterità.Starting from the first researches of Laboratorio Incontri Generazionali of Università degli Studi di Milano (1998) on “Computer Languages and Inter-generational Communication” and assuming the first point: “socialize generation, socializing institutions” (A. Albanese, 1999, 2000), studies of intergenerational tourism born in North of Italy area.
We will focus on the efficacy of the “Nonni e Nipoti” program, oriented on creation and experimentation of specified “meeting” and “contact” situations (based on touristic-cultural paths) among people from different generations (teenagers vs elderly) in the host community of Sfruz (Tn) and in others communities.
Intergenerational tourism is a form of social tourism, pedagogical tourism, sustainable tourism. Holidays promote health, social and individual weel-being; encourage socialization of different generations and prevent the awkwardness of youth and of old age
New nanoparticles based on cholesterol-poly(amidoamine) and b-Cyclodextrin- poly(4-acryloylmorpholine) conjugates as drug carriers
No full textPoly(amidoamine) Hydrogels as Scaffolds for Cell Culturing and Conduits for Peripheral Nerve Regeneration
Get PDFBiodegradable and biocompatible poly(amidoamine)-(PAA-) based hydrogels have been considered for different tissue engineering applications. First-generation AGMA1 hydrogels, amphoteric but prevailing cationic hydrogels containing carboxylic and guanidine groups as side substituents, show satisfactory results in terms of adhesion and proliferation properties towards different cell lines. Unfortunately, these hydrogels are very swellable materials, breakable on handling, and have been found inadequate for other applications. To overcome this problem, second-generation AGMA1 hydrogels have been prepared adopting a new synthetic method. These new hydrogels exhibit good biological properties in vitro with satisfactory mechanical characteristics. They are obtained in different forms and shapes and successfully tested in vivo for the regeneration of peripheral nerves. This paper reports on our recent efforts in the use of first-and second-generation PAA hydrogels as substrates for cell culturing and tubular scaffold for peripheral nerve regeneration
On the analytical solution for the slewing flexible beam-like system: Analysis of the linear part of the perturbed problem
No full textMade available in DSpace on 2019-09-12T16:57:16Z (GMT). No. of bitstreams: 0
Previous issue date: 2008Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)The dynamical system investigated in this work is a nonlinear flexible beam-like structure in slewing motion. Non-dimensional and perturbed governing equations of motion are presented. The analytical solution for the linear part of these perturbed equations for ideal and for non-ideal cases are obtained. This solution is necessary for the investigation of the complete weak nonlinear problem where all nonlinearities are small perturbations around a linear known solution. This investigation shall help the analyst in the modelling of dynamical systems with structure-actuator interactions.[Fenili, Andre] Universidade de Taubaté (Unitau), Dept Engn Mech[Balthazar, Jose Manoel] UNESP, Dept Estatist Matemat Aplicada & Computacao, Rio Claro, SP, Brazil[Balthazar, Jose Manoel] Univ Estadual Campinas, Mech Design Dept, Campinas, SP, Brazi
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