124 research outputs found
Characterization of PCR-RFLP profiles on partial co1 geneof harpacticoid copepod, Leptocaris canariensis (Lang, 1965), UMT strain
No full textLeptocaris canariensis (Lang, 1965) is a benthic copepod that belongs to Order Harpacticoida. It is essential in aquaculture sector due to smaller size and good nutritional content suitable for the growth and survival of the fish larvae. It is also an important prey for other copepod and predators to mosquito larvae. The identification of the copepod species was done based on morphological characteristic only which is time consuming and need to be done on adult copepod only. There is no molecular study being done on L. canariensis itself
Molecular study of psammobia rostratus (ambal goyang) from Buntal Bay, Sarawak
No full textPcannnohia rostratus (Antbai Govang) is one of the species of clams that spread along Buntal bay area. P.
rostratus is filter feeders that plays important role in ecological services. They are harvested by local people from August to March during low tide either for domestic consumption or side income. The aims of this study were to amplify and sequence the 16S rRNA gene, COI gene, ITS gene and ITS1 gene, documented the PCR-RFLP
restriction profile of ITS gene. The modified CTAB DNA extraction method and modified Phenol Chloroform
DNA extraction method were used to extract the total genomics DNA of about 33 samples of P. rostratus from
Buntal bay area. In this study, the amplification of the mitochondrial gene, 16S rRNA gene generated fragment
size approximately 550 bp whereas the COI gene only generated fragment approximately 350 bp. For nuclear
DNA gene, the size of ITS gene are approximately 1000 bp and no positive result yielded for the amplification of
ITS I gene. After PCR, the 16S rRNA gene, COI gene and ITS gene were purified and sent to commercial
company for sequencing but the sequencing result was noisy and cannot be used for further analysis. The PCR
product of ITS gene were subjected for PCR-RFLP analysis using 10 restriction enzymes (REs) namely Csp61,
Hindlll, Tagl, Mholl, BsuRI, MnII, MspI (Hpall), Mbol, Ail and Rsal. Excluding Csp6I and Hindlll, all REs
successfully revealed the restriction profile
The Post-Research Ethics Analysis (PREA) Project
No full textAUTHOR AFFILIATION: Dónal O'Mathúna, The Ohio State University, United States, and Dublin City University, Ireland, [email protected] media can be accessed here: http://streaming.osu.edu/knowledgebank/PREA/PREA_Session4_OMathuna_20190325.mp4A series of presentations provide an overview of the PREA project and preliminary findings. An initial scoping review led to the Research Ethics Tool commissioned by the project funders, R2HC. Then a more thorough PREA Systematic Review was conducted, led by Mr Steven Martin of Anglia Ruskin University, UK. The methods and search results are presented, followed by an overview of the 13 Themes identified in the analysis. Then Prof Tine Van Bortel introduced the qualitative research component, beginning with the training for the in-country interviewers. Mr Nawaraj Upadhaya of HealthNet TPO discusses the coordination of the interviews in Nepal, Afghanistan and South Sudan. Dr Yimtu Mulate, Addis Ababa University conducted the interviews in Ethiopia. The qualitative analysis was discussed by Prof Van Bortel and Dr Ainul Hanafiah of the University of East London, UK. The methods of the analysis and the 10 Themes identified were presented, as well as overall conclusions from the findings
Infestation of parasitic rhizocephalan barnacles Sacculina beauforti (Cirripedia, Rhizocephala) in edible mud crab, Scylla olivacea
No full textScreening of mud crab genus Scylla was conducted in four locations (Marudu Bay, Lundu, Taiping, Setiu) representing Malaysia. Scylla olivacea with abnormal primary and secondary sexual characters were prevalent (approximately 42.27% of the local screened S. olivacea population) in Marudu Bay, Sabah. A total of six different types of abnormalities were described. Crabs with type 1 and type 3 were immature males, type 2 and type 4 were mature males, type 5 were immature females and type 6 were mature females. The abdomen of all crabs with abnormalities were dented on both sides along the abdomen’s middle line. Abnormal crabs showed significant variation in their size, weight, abdomen width and/or gonopod or pleopod length compared to normal individuals. The mean body weight of abnormal crabs (type 1–5) were higher than normal crabs with smaller body size, while females with type 6 abnormality were always heavier than the normal counterparts at any given size. Sacculinid’s externa were observed in the abdomen of crabs with type 4 and type 6 abnormalities. The presence of embryos within the externa and subsequent molecular analysis of partial mitochondrial COI region confirmed the rhizocephalan parasite as Sacculina beauforti. Future in-depth descriptions of the life cycle and characteristics of S. beauforti are recommended as it involves a commercially important edible crab species and the effect on human health from the consumption of crabs is of crucial concern
Non-indigenous giant mud crab, Scylla serrata (Forskål, 1775) (Crustacea: Brachyura: Portunidae) in Malaysian coastal waters: a call for caution
No full text
Rekonstruksi Kepemimpinan Pendidikan Umar bin Abdul Aziz: Aktualisasi Politik Pengembangan Pendidikan
No full textThe article aims to explore the politics of education at Umar bin Abdul Aziz's time and then reconstruct it with the realities of contemporary education. In examining this, the author uses qualitative methods with the type of research library. The search results show that Umar bin Abdul Aziz developed an educational aspect focusing on three areas, namely 1) family education, 2) formal education, and 3) community education. These three development fields are still very relevant if reconstructed and contextualized with current education development
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