1,721,068 research outputs found
Determination of mitochondrial number and function
No full textMitochondria transplantation is considered a potential therapeutic method that can be used in diseases related to mitochondrial dysfunction, such as neurodegenerative diseases, toxic lesions, ischemia, and cardiovascular diseases. To achieve successful transplantation, whether in vivo or in vitro, it is of paramount importance to accurately determine the number and quality of isolated mitochondria. The structural integrity of isolated mitochondria must be carefully evaluated, as it directly correlates with the organelles' ability to maintain transmembrane potential and thus promote their bioenergetic function by effectively synthesizing ATP
On the break in the arrhenius plot of membrane-bound ubiquinol-cytochrome c reductase: an artifact diagnostic for diffusion control of cytochrome c interaction
No full textIsolation process for mitochondrial transplantation
No full textMitochondrial transplantation is an innovative therapeutic approach for the treatment of mitochondrial dysfunction associated with a variety of metabolic, neurodegenerative and cardiovascular diseases. In this procedure, healthy mitochondria are isolated and transferred into damaged or dysfunctional cells to restore normal cellular bioenergetics. Recent advances have shown the feasibility of mitochondrial transplantation in preclinical and clinical settings, demonstrating its potential to improve outcomes in conditions such as myocardial infarction, neurodegenerative disorders, and acute kidney injury. In this contest, the isolation method for mitochondrial transplantation is crucial. High quality, pure mitochondria are essential to ensure that the transplanted organelles function correctly and integrate well into the recipient cells. In addition, efficient isolation techniques are required to obtain a sufficient number of mitochondria for transplantation. Low yield methods may not provide enough mitochondria for effective treatment. Several isolation techniques are currently used, including differential centrifugation, density gradient centrifugation and fractionated mitochondrial magnetic separation. Each method has its own advantages and limitations, and the choice of method often depends on the specific requirements of the application, such as the type of cells targeted and the disease to be treated. A key factor to consider when choosing the isolation method is the isolation time. Rapid isolation methods are preferred for the isolation of mitochondria from tissue biopsies. This review summarizes the current state of research on mitochondrial transplantation, highlights the main methods for isolating mitochondria and discusses their potential clinical applications in the field of regenerative medicine
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Storage of isolated mitochondria
No full textAfter extraction and quantification steps, the most intriguing challenge in preparing mitochondria for transplantation of these organelles is the preservation of the material isolated from different tissues. It is essential to maintain these organelles in a viable condition without losing the biological characteristics of the tissue of origin. After isolation and quantification, various methods, when performed following proper protocols, lead to the last step of preparation mitochondria ready for use. Two main methods are used for preservation of mitochondria after isolation: cold storage and cryopreservation. Current methodological approaches in mitochondrial transplantation techniques suggest using freshly prepared isolated mitochondria from different tissues; this could be a limitation for mitochondrial transplantation techniques, and further efforts in methodological research should be aimed at overcoming this problem
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