2,106 research outputs found
Ty Matson: Senior Recital, Trombone
No full textThis Senior Honors Project is entitled “Ty Matson: Senior Recital, Trombone.” The author of the project is Ty Matson. The Senior Honors Project was the preparation and performance of my senior trombone recital, with program notes to accompany the traditional program list of pieces. The trombone recital was comprised of seven pieces of varied styles, musical eras, and composers. The program notes that were distributed in addition to the program consisted of background information on the composers as well as the compositions themselves. The recital, not including intermission, lasted approximately 47 minutes
Ty Matson: Senior Recital , Trombone
No full text"This Senior Honors Project is entitled ""Ty Matson: Senior Recital , Trombone."" The author of the project is Ty Matson. The Senior Honors Project was the preparation and performance of my senior trombone recital , with program notes to accompany the traditional program list of pieces. The trombone recital was comprised of seven pieces of varied styles , musical eras , and composers. The program notes that were distributed in addition to the program consisted of background information on the composers as well as the compositions themselves. The recital , not including intermission , lasted approximately 47 minutes.
Ty Matson: Senior Recital, Trombone
No full textThis Senior Honors Project is entitled “Ty Matson: Senior Recital, Trombone.” The author of the project is Ty Matson. The Senior Honors Project was the preparation and performance of my senior trombone recital, with program notes to accompany the traditional program list of pieces. The trombone recital was comprised of seven pieces of varied styles, musical eras, and composers. The program notes that were distributed in addition to the program consisted of background information on the composers as well as the compositions themselves. The recital, not including intermission, lasted approximately 47 minutes
Osmotic pressure and virial coefficients of star and comb polymer solutions: Dissipative particle dynamics
No full textHand, Foot and Mouth Disease Complicated with Central Nervous System Involvement in Taiwan in 1980-1981
No full textThe Creation of the Role of Edward Ferrars in Sense and Sensibility
Get PDFThis document is a thesis submitted in partial fulfillment of the Master of Fine Arts degree in Acting. It is a detailed account of author Ty Hudson’s artistic process in creating the role of Edward Ferrars in Sense and Sensibility as a part of Minnesota State University, Mankato’s mainstage theatre season in the winter of 2021. The thesis chronicles the actor’s artistic process from pre-production through performance in five chapters: a preproduction analysis, an historical and critical perspective, a rehearsal and performance journal, a post-production analysis and a process development analysis. Appendices and works cited are included
Antitumor activity of TY-011 against gastric cancer by inhibiting Aurora A, Aurora B and VEGFR2 kinases
No full textAbstract Background Overexpression of Aurora A and B has been reported in a wide range of tumor types, including gastric cancer. Anti-angiogenesis has been considered as an important therapeutic modality in advanced gastric cancer. Here we identified a novel compound TY-011 with promising antitumor activity by targeting mitotic kinases (Aurora A and B) and angiogenic receptor tyrosine kinase (VEGFR2). Methods HTRF® KinEASE™ assay was used to detect the effect of TY-011 against Aurora A, Aurora B and VEGFR2 activities. Docking simulation study was performed to predict the binding mode of TY-011 with Aurora A and B kinases. CCK-8 assay was used to test cell growth. Cell cycle and cell apoptosis was analyzed by flow cytometry. Gastric cancer cell xenograft mouse models were used for in vivo study. TUNEL kit was used to determine the apoptosis of tumor tissues. Immunohistochemistry analysis and HUVEC tube formation assay were performed to determine the anti-angiogenesis ability. Immunofluorescence and western blot were used to test protein expression. Results TY-011 was identified as a potential Aurora A and B inhibitor by HTRF® KinEASE™ assay. It effectively inhibited cellular Aurora A and B activities in a concentration-dependent manner. TY-011 occupied the ATP-binding site of both Aurora A and B kinases. TY-011 demonstrated prominent inhibitory effects on proliferation of gastric cancer cells. TY-011 treatment induced an obvious accumulation of cells at G2/M phase and a modest increase of cells with >4 N DNA content, which then underwent apoptosis. Meaningfully, orally administration of TY-011 demonstrated superior efficacy against the tumor growth in gastric cancer cell xenograft, with ~90% inhibition rate and 100% tumor regression at 9 mg/kg dose, and TY-011 did not affect the body weight of mice. Interestingly, we observed that TY-011 also antagonized tumor angiogenesis by targeting VEGFR2 kinase. Conclusions These results indicate that TY-011 is a well-tolerated, orally active compound that targets mitosis and angiogenesis in tumor growth, and provides strong preclinical support for use as a therapeutic for human gastric cancers
Cutting'aesthetic teeth' : Flannery O'Connor's habit of art
Get PDFDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Comunicação e ExpressãoEste trabalho foi sugerido pela afirmação de Flannery O'Connor que sua "dedicação estética" nasceu através do contato com Art and Scholasticism de Jacques Maritain. O propósito foi chegar a uma interpretação do sentido da frase. Uma investigação detalhada foi feita do conteúdo de Art and Scholasticism, posteriormente contrastada com os resultados de uma pesquisa feita em seus ensaios e suas cartas, o que revelou numerosos ecos de diversos trechos constando no texto de Maritain. Três pontos principais foram escolhidos como critérios na análise do hábito artístico de O'Connor: 1) a prática de arte implica uma luta; 2) a arte somente pode ser percebida pelos sentidos; e 3) a prática de arte exige do artista a dedicação indivisa à obra nascente. O estudo conclui que, para O'Connor, o brotar da dentição estética, através da leitura de Art and Scholasticism, significou que, ao perceber na análise da natureza da arte algo com que podia concordar, ela reconheceu tanto sua própria capacidade de tornar-se uma artista literária, quanto sua vontade de assumir a tarefa de desenvolver em sua pessoa o hábito de arte
An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol
No full textBackground: Mutagenesis plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which are more tractable for biophysical techniques. The ability to quickly and specifically mutate a residue(s) in protein is important for mechanistic and functional studies. Although many site-directed mutagenesis methods have been developed, a simple, quick and multi-applicable method is still desirable. Results: We have developed a site-directed plasmid mutagenesis protocol that preserved the simple one step procedure of the QuikChange (TM) site-directed mutagenesis but enhanced its efficiency and extended its capability for multi-site mutagenesis. This modified protocol used a new primer design that promoted primer-template annealing by eliminating primer dimerization and also permitted the newly synthesized DNA to be used as the template in subsequent amplification cycles. These two factors we believe are the main reasons for the enhanced amplification efficiency and for its applications in multi-site mutagenesis. Conclusion: Our modified protocol significantly increased the efficiency of single mutation and also allowed facile large single insertions, deletions/truncations and multiple mutations in a single experiment, an option incompatible with the standard QuikChange (TM). Furthermore the new protocol required significantly less parental DNA which facilitated the DpnI digestion after the PCR amplification and enhanced the overall efficiency and reliability. Using our protocol, we generated single site, multiple single-site mutations and a combined insertion/deletion mutations. The results demonstrated that this new protocol imposed no additional reagent costs (beyond basic QuikChange T) but increased the overall success rates.Peer reviewe
Alleviating D-Galactose-Induced Aging in Mice by Modulating Gut-Liver Axis Using Lactiplantibacillus plantarum TY-Y10
No full textOxidative stress is closely linked to aging. Probiotics, whether viable or heat-inactivated, have shown antioxidant properties; however, their effect and mechanism of action in reducing oxidative stress during aging remains underexplored. This study examined the effects of viable and heat-inactivated Lactiplantibacillus plantarum TY-Y10 (L. plantarum TY-Y10) on D-galactose (D-gal)-induced aging in mice, aiming to uncover potential anti-aging mechanisms. Mice were induced to age with D-gal injections, then treated with sodium ascorbate (positive control) or varying doses of L. plantarum TY-Y10 for eight weeks. After treatment, oxidative stress markers, gut microbiota, and liver health were analyzed. Results showed that L. plantarum TY-Y10 decreased malondialdehyde (MDA) and inflammatory markers while increasing antioxidant levels (glutathione, superoxide dismutase, catalase and glutathione peroxidase). Liver damage was reduced, and expression of Nrf2 and related antioxidant enzymes improved. Additionally, L. plantarum TY-Y10 enhanced the abundance of short-chain fatty acid-producing bacteria, boosting fecal short-chain fatty acid levels. In short, both viable and heat-inactivated L. plantarum TY-Y10 mitigated oxidative stress in aging mice by modulating gut microbiota and activating liver antioxidant pathways through the gut-liver axis
- …
