1,721,358 research outputs found
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Cloning and functional characterization of two putative proteases in Helicobacter pylori
No full text幽門螺旋桿菌 (Helicaobacter pylori) 寄生於人類胃中，目前已知與許多胃部疾病相關，但詳細的致病機制與毒性仍需更進一步的釐清和證明。在微生物中，蛋白酶除了在生存上是必備的成分外，為了能夠寄生在宿主體內，微生物將會發展出針對宿主產生破壞與毒性的功能，例如在營養的取得、毒性因子的合成、分解宿主細胞特定蛋白以便控制宿主的反應以及菌體本身蛋白量與質的控制等。幽門螺旋桿菌26695菌株中有33個基因已被預測出具有蛋白酶的功能，其中我們挑選了tagE、htrA這兩個基因進行探討，這是因為之前的研究並未在蛋白酶功能部分多所著墨，此外也發現這兩個基因轉錄出來的mRNA在酸性環境刺激下都有上升的趨勢。我們在幽門螺旋桿菌NTUH-C1菌株選殖這兩個基因，由於htrA基因在幽門螺旋桿菌中已被證明是essential gene，所以我們僅製作tagE突變株來看對幽門螺旋桿菌的影響。初步發現tagE突變株在生長複製上的表現與野生株無太大的差異，但是經由初步的統計結果及穿透式電子顯微鏡的觀察，我們可以看到突變株的菌體長度較野生株短。之後，為了進一步確定其蛋白酶的活性與功能，我們在大腸桿菌中使用不同的載體，經過表現和純化的過程後得到TagE與HtrA重組蛋白。最後，我們以zymogram這個技術確定HtrA蛋白的確具有蛋白酶的活性，能夠分解受質β-casein ﹔而TagE蛋白則沒有活性的表現。Helicobacter pylori colonizes in human stomach and is recognized as a cause of many gastric diseases. However, the mechanism of pathogenesis and related toxic factors need further study and identification. In microbes, proteases play important roles in many parts of their life cycles. In order to colonize the host, microbes often use their protease activity to be virulence factors, including the acquisition of nutrient, biosynthesis of pathogenetic factors, cleavage key host proteins for mediation of the host response, and the protein quality control. There are 33 genes which have putative protease function in Helicobacter pylori 26695. In this study, we tried to characterize the TagE、HtrA protein functions, because their protease activity is not yet clarify and mRNA expression in high level under acidic stress. We cloned the tagE、htrA genes from Helicobacter pylori NTUH-C1. htrA is proved as an essential gene, so only the tagE-knockout strain was constructed. Then, we compared the wild type and tagE-knockout strain, it’s no distint difference in the growth and replication, but under the transmission electron microscopy and statistics results we observed the phenotype of the tagE-knockout strain has decreased elongation. Moreover, to identify the protease activity, we expressed and purified the TagE and HtrA recombinant protein. Finally, zymogram confirmed that the htrA had the protease activity to degrade β-casein, but tagE didn’t.目錄………………………………………………………………………………......1
中文摘要……………………………………………………………………………..3
英文摘要……………………………………………………………………………..4
第一章 前言…………………………………………………………………………5
第二章 研究目的……………………………………………………………………11
第三章 實驗材料與方法
3.1 實驗材料
3.1.1 幽門螺旋桿菌 (H. pylori) 菌株………………………………………...12
3.1.2 大腸桿菌 (E. coli) 菌株………………………………………………...12
3.1.3 培養基……………………………………………………………………13
3.1.4 載體 (vector)…………………………………………………………….14
3.1.5 引子………………………………………………………………………15
3.1.6 抗體………………………………………………………………………15
3.2 實驗方法
3.2.1 tagE基因的選殖與分析…………………………………………………16
3.2.2 htrA 基因的選殖與分析………………………………………………...18
3.2.3 以轉位作用得到幽門螺旋桿菌tagE突變株…………………………18
3.2.4 NTUH-C1野生株與tagE突變株生長曲線與外在型態的比較………..20
3.2.5 獲得TagE重組蛋白……………………………………………………21
3.2.6 製作針對TagE蛋白的專一性抗體……………………………………24
3.2.7 獲得HtrA重組蛋白……………………………………………………25
3.2.8 tagE基因的蛋白酶活性分析……………………………………………26
3.2.9 htrA基因的蛋白酶活性確立……………………………………………26
第四章 實驗結果
4.1 tagE基因的選殖與分析…………………………………………………...27
4.2 htrA 基因的選殖與分析…………………………………………………..28
4.3 以轉位作用得到幽門螺旋桿菌tagE突變株……………………………29
4.4 NTUH-C1野生株與tagE突變株生長曲線與外在型態的比較………….29
4.5 獲得TagE重組蛋白………………………………………………………30
4.6 製作針對TagE蛋白的專一性抗體………………………………………31
4.7 獲得HtrA重組蛋白………………………………………………………32
4.8 tagE基因的蛋白酶活性分析……………………………………………32
4.9 htrA基因的蛋白酶活性確立……………………………………………33
第五章 結果討論……………………………………………………………………34
第六章 圖表
圖一A tagE基因定序的結果……………………………………………………40
圖一B TagE與peptidase subfamily M23B蛋白catalytic residues的比對…......41
圖二A htrA基因定序的結果……………………………………………………42
圖二B HtrA與peptidase subfamily S1C蛋白catalytic residues的比對……….43
圖三 tagE突變株的建立………………………………………………………..44
圖四 野生株與tagE突變株pH7.2與5.5環境下生長曲線的比較……………45
圖五 野生株與tagE突變株外在型態的比較…………………………………46
圖六 野生株與tagE突變株菌體長度量化的比較……………………………47
圖七 野生株與tagE突變株於穿透式電子顯微鏡下外在型態的比較………48
圖八 TagE重組蛋白表現與條件嘗試…………………………………………49
圖九 TagE重組蛋白的純化……………………………………………………50
圖十 Anti-TagE Ab的測試……………………………………………………...51
圖十一 HtrA重組蛋白表現與條件嘗試………………………………………52
圖十二 HtrA 重組蛋白的純化…………………………………………………53
圖十三 Zymogram—TagE蛋白活性分析……………………………………54
圖十四 Zymogram—HtrA蛋白活性確立……………………………………55
第七章 附圖
附圖一 幽門螺旋桿菌26695菌株的HP1543基因.............................................56
附圖二 幽門螺旋桿菌26695菌株的HP1019基因……………………………57
附圖三 full length TagE重組蛋白表現質體的建構……………………………58
附圖四 HtrA重組蛋白表現質體的建構………………………………………59
第八章 參考文獻……………………………………………………………………6
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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